Electric cell-substrate impedance sensing in biocompatibility research
In this paper, the possibility of using cell culture impedance measurements to assess the biocompatibility of a material in contact with cells was analyzed. For this purpose, the Electric Cell-substrate Impedance Sensing (ECIS) method and a commercial measuring device were used. The test substrates...
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| Format: | Article |
| Language: | English |
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Sciendo
2021-12-01
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| Series: | Journal of Electrical Bioimpedance |
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| Online Access: | https://doi.org/10.2478/joeb-2021-0019 |
| _version_ | 1811220194847096832 |
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| author | Kociubiński Andrzej |
| author_facet | Kociubiński Andrzej |
| author_sort | Kociubiński Andrzej |
| collection | DOAJ |
| description | In this paper, the possibility of using cell culture impedance measurements to assess the biocompatibility of a material in contact with cells was analyzed. For this purpose, the Electric Cell-substrate Impedance Sensing (ECIS) method and a commercial measuring device were used. The test substrates with thin-film electrodes made of various metals were prepared using the magnetron sputtering method. The choice of metals was dictated by their varying degrees of biocompatibility. Cultures of mouse fibroblasts were cultured on the prepared substrates. The experiment showed that the complete cycle of culture from attachment and reproduction to apoptosis occurred. The results obtained indicate that it is possible to use the ECIS method to study the influence of metal on cell culture activity. |
| first_indexed | 2024-04-12T07:37:29Z |
| format | Article |
| id | doaj.art-070fa89fe6cb498bb98072a058c0a047 |
| institution | Directory Open Access Journal |
| issn | 1891-5469 |
| language | English |
| last_indexed | 2024-04-12T07:37:29Z |
| publishDate | 2021-12-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Journal of Electrical Bioimpedance |
| spelling | doaj.art-070fa89fe6cb498bb98072a058c0a0472022-12-22T03:41:54ZengSciendoJournal of Electrical Bioimpedance1891-54692021-12-0112116316810.2478/joeb-2021-0019Electric cell-substrate impedance sensing in biocompatibility researchKociubiński Andrzej0Department of Electronics and Information Technology, Lublin University of Technology, Lublin, PolandIn this paper, the possibility of using cell culture impedance measurements to assess the biocompatibility of a material in contact with cells was analyzed. For this purpose, the Electric Cell-substrate Impedance Sensing (ECIS) method and a commercial measuring device were used. The test substrates with thin-film electrodes made of various metals were prepared using the magnetron sputtering method. The choice of metals was dictated by their varying degrees of biocompatibility. Cultures of mouse fibroblasts were cultured on the prepared substrates. The experiment showed that the complete cycle of culture from attachment and reproduction to apoptosis occurred. The results obtained indicate that it is possible to use the ECIS method to study the influence of metal on cell culture activity.https://doi.org/10.2478/joeb-2021-0019bioimpedancebiocompatibilitycell culturetechnology |
| spellingShingle | Kociubiński Andrzej Electric cell-substrate impedance sensing in biocompatibility research Journal of Electrical Bioimpedance bioimpedance biocompatibility cell culture technology |
| title | Electric cell-substrate impedance sensing in biocompatibility research |
| title_full | Electric cell-substrate impedance sensing in biocompatibility research |
| title_fullStr | Electric cell-substrate impedance sensing in biocompatibility research |
| title_full_unstemmed | Electric cell-substrate impedance sensing in biocompatibility research |
| title_short | Electric cell-substrate impedance sensing in biocompatibility research |
| title_sort | electric cell substrate impedance sensing in biocompatibility research |
| topic | bioimpedance biocompatibility cell culture technology |
| url | https://doi.org/10.2478/joeb-2021-0019 |
| work_keys_str_mv | AT kociubinskiandrzej electriccellsubstrateimpedancesensinginbiocompatibilityresearch |