Diseño de un medio para la producción de un co-cultivo de bacterias fosfato solubilizadoras con actividad fosfatasa
Design of a culture media for the production of a phosphate-solubilizing bacteria co-culture. Objective. To design a complexculture media for the production of biomass and acid phosphatases from phosphate-solubilizing bacteria isolated from soil. Materialsand methods. Phosphate-solubilizing bacteria...
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| Format: | Article |
| Language: | English |
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Pontificia Universidad Javeriana
2012-04-01
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| Series: | Universitas Scientiarum |
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| Online Access: | http://revistas.javeriana.edu.co/index.php/scientarium/article/view/2481/1760 |
| _version_ | 1818194830176550912 |
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| author | Jimena Paola Angulo-Cortés Viviana Gutiérrez-Romero |
| author_facet | Jimena Paola Angulo-Cortés Viviana Gutiérrez-Romero |
| author_sort | Jimena Paola Angulo-Cortés |
| collection | DOAJ |
| description | Design of a culture media for the production of a phosphate-solubilizing bacteria co-culture. Objective. To design a complexculture media for the production of biomass and acid phosphatases from phosphate-solubilizing bacteria isolated from soil. Materialsand methods. Phosphate-solubilizing bacteria were isolated from oil palm crop soil samples and selected on SMRS1 agar, which werethen assessed with antagonism tests to verify their aptitude to form a co-culture. A Box-Behnken experimental design was applied toevaluate the effect of each one of the culture media components on the production of biomass and phosphatase enzymes at a laboratoryscale. Finally, microbial growth and enzyme production curves were carried out in order to determine their production times. Results.Five phosphate-solubilizing bacterial strains were isolated and three of them were selected based on their solubilization indices.These Gram negative strains with bacillus morphology were identified as A, B and C; their solubilization indices were 2.03, 2.12, and2.83, respectively. According to the ANOVA analyses for the Box-Behnken design, the only factor which had a significant effect onthe phosphatase activity (p<0.01) was hydrolyzed yeast, and the formulation that generated the highest biomass concentration andphosphatase activity (p<0.01) contained 10, 15 and 2.5 gL-1 of phosphoric rock, sucrose and hydrolyzed yeast, respectively. After 24hours of incubation at 100 rpm, the highest values of biomass and phosphatase activity were obtained: 11.8 logarithmic units of CFUand 12.9 phosphatase units. Conclusion. We determined that the culture media based on phosphoric rock 10 gL-1, hydrolyzed yeast 2.5gL-1 and commercial sucrose 15 gL-1 was ideal for the production of biomass and phosphatases by the strains evaluated; likewise, weproved that the hydrolyzed yeast was the only factor significantly influential for the production of phosphatases. |
| first_indexed | 2024-12-12T01:08:31Z |
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| id | doaj.art-0710417583804495a9c7ba827120a951 |
| institution | Directory Open Access Journal |
| issn | 0122-7483 2027-1352 |
| language | English |
| last_indexed | 2024-12-12T01:08:31Z |
| publishDate | 2012-04-01 |
| publisher | Pontificia Universidad Javeriana |
| record_format | Article |
| series | Universitas Scientiarum |
| spelling | doaj.art-0710417583804495a9c7ba827120a9512022-12-22T00:43:32ZengPontificia Universidad JaverianaUniversitas Scientiarum0122-74832027-13522012-04-011714352Diseño de un medio para la producción de un co-cultivo de bacterias fosfato solubilizadoras con actividad fosfatasaJimena Paola Angulo-CortésViviana Gutiérrez-RomeroDesign of a culture media for the production of a phosphate-solubilizing bacteria co-culture. Objective. To design a complexculture media for the production of biomass and acid phosphatases from phosphate-solubilizing bacteria isolated from soil. Materialsand methods. Phosphate-solubilizing bacteria were isolated from oil palm crop soil samples and selected on SMRS1 agar, which werethen assessed with antagonism tests to verify their aptitude to form a co-culture. A Box-Behnken experimental design was applied toevaluate the effect of each one of the culture media components on the production of biomass and phosphatase enzymes at a laboratoryscale. Finally, microbial growth and enzyme production curves were carried out in order to determine their production times. Results.Five phosphate-solubilizing bacterial strains were isolated and three of them were selected based on their solubilization indices.These Gram negative strains with bacillus morphology were identified as A, B and C; their solubilization indices were 2.03, 2.12, and2.83, respectively. According to the ANOVA analyses for the Box-Behnken design, the only factor which had a significant effect onthe phosphatase activity (p<0.01) was hydrolyzed yeast, and the formulation that generated the highest biomass concentration andphosphatase activity (p<0.01) contained 10, 15 and 2.5 gL-1 of phosphoric rock, sucrose and hydrolyzed yeast, respectively. After 24hours of incubation at 100 rpm, the highest values of biomass and phosphatase activity were obtained: 11.8 logarithmic units of CFUand 12.9 phosphatase units. Conclusion. We determined that the culture media based on phosphoric rock 10 gL-1, hydrolyzed yeast 2.5gL-1 and commercial sucrose 15 gL-1 was ideal for the production of biomass and phosphatases by the strains evaluated; likewise, weproved that the hydrolyzed yeast was the only factor significantly influential for the production of phosphatases.http://revistas.javeriana.edu.co/index.php/scientarium/article/view/2481/1760bio-inoculantsphosphate solubilizing microorganismsphosphatase activityBox Behnken design |
| spellingShingle | Jimena Paola Angulo-Cortés Viviana Gutiérrez-Romero Diseño de un medio para la producción de un co-cultivo de bacterias fosfato solubilizadoras con actividad fosfatasa Universitas Scientiarum bio-inoculants phosphate solubilizing microorganisms phosphatase activity Box Behnken design |
| title | Diseño de un medio para la producción de un co-cultivo de bacterias fosfato solubilizadoras con actividad fosfatasa |
| title_full | Diseño de un medio para la producción de un co-cultivo de bacterias fosfato solubilizadoras con actividad fosfatasa |
| title_fullStr | Diseño de un medio para la producción de un co-cultivo de bacterias fosfato solubilizadoras con actividad fosfatasa |
| title_full_unstemmed | Diseño de un medio para la producción de un co-cultivo de bacterias fosfato solubilizadoras con actividad fosfatasa |
| title_short | Diseño de un medio para la producción de un co-cultivo de bacterias fosfato solubilizadoras con actividad fosfatasa |
| title_sort | diseno de un medio para la produccion de un co cultivo de bacterias fosfato solubilizadoras con actividad fosfatasa |
| topic | bio-inoculants phosphate solubilizing microorganisms phosphatase activity Box Behnken design |
| url | http://revistas.javeriana.edu.co/index.php/scientarium/article/view/2481/1760 |
| work_keys_str_mv | AT jimenapaolaangulocortes disenodeunmedioparalaproducciondeuncocultivodebacteriasfosfatosolubilizadorasconactividadfosfatasa AT vivianagutierrezromero disenodeunmedioparalaproducciondeuncocultivodebacteriasfosfatosolubilizadorasconactividadfosfatasa |