A Single Nonsynonymous Substitution in the RNA-Dependent RNA Polymerase of <i>Potato virus Y</i> Allows the Simultaneous Breakdown of Two Different Forms of Antiviral Resistance in <i>Capsicum annuum</i>
The dominant <i>Pvr4</i> gene in pepper (<i>Capsicum annuum</i>) confers resistance to members of six potyvirus species, all of which belong to the <i>Potato virus Y</i> (PVY) phylogenetic group. The corresponding avirulence factor in the PVY genome is the NIb cis...
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MDPI AG
2023-04-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/15/5/1081 |
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| author | Benoît Moury Thierry Michon Vincent Simon Alain Palloix |
| author_facet | Benoît Moury Thierry Michon Vincent Simon Alain Palloix |
| author_sort | Benoît Moury |
| collection | DOAJ |
| description | The dominant <i>Pvr4</i> gene in pepper (<i>Capsicum annuum</i>) confers resistance to members of six potyvirus species, all of which belong to the <i>Potato virus Y</i> (PVY) phylogenetic group. The corresponding avirulence factor in the PVY genome is the NIb cistron (i.e., RNA-dependent RNA polymerase). Here, we describe a new source of potyvirus resistance in the Guatemalan accession <i>C. annuum</i> cv. PM949. PM949 is resistant to members of at least three potyvirus species, a subset of those controlled by <i>Pvr4</i>. The F<sub>1</sub> progeny between PM949 and the susceptible cultivar Yolo Wonder was susceptible to PVY, indicating that the resistance is recessive. The segregation ratio between resistant and susceptible plants observed in the F<sub>2</sub> progeny matched preferably with resistance being determined by two unlinked recessive genes independently conferring resistance to PVY. Inoculations by grafting resulted in the selection of PVY mutants breaking PM949 resistance and, less efficiently, <i>Pvr4</i>–mediated resistance. The codon substitution E<sub>472</sub>K in the NIb cistron of PVY, which was shown previously to be sufficient to break <i>Pvr4</i> resistance, was also sufficient to break PM949 resistance, a rare example of cross-pathogenicity effect. In contrast, the other selected NIb mutants showed specific infectivity in PM949 or <i>Pvr4</i> plants. Comparison of <i>Pvr4</i> and PM949 resistance, which share the same target in PVY, provides interesting insights into the determinants of resistance durability. |
| first_indexed | 2024-03-11T03:14:03Z |
| format | Article |
| id | doaj.art-07132da6e3b949b09a7aae5b4a56796e |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-11T03:14:03Z |
| publishDate | 2023-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-07132da6e3b949b09a7aae5b4a56796e2023-11-18T03:38:41ZengMDPI AGViruses1999-49152023-04-01155108110.3390/v15051081A Single Nonsynonymous Substitution in the RNA-Dependent RNA Polymerase of <i>Potato virus Y</i> Allows the Simultaneous Breakdown of Two Different Forms of Antiviral Resistance in <i>Capsicum annuum</i>Benoît Moury0Thierry Michon1Vincent Simon2Alain Palloix3INRAE, Pathologie Végétale, 84140 Montfavet, FranceUMR Biologie du Fruit et Pathologie, INRAE, Université de Bordeaux, CS 20032, 33882 Villenave d’Ornon, FranceINRAE, Pathologie Végétale, 84140 Montfavet, FranceINRAE, GAFL, 84140 Montfavet, FranceThe dominant <i>Pvr4</i> gene in pepper (<i>Capsicum annuum</i>) confers resistance to members of six potyvirus species, all of which belong to the <i>Potato virus Y</i> (PVY) phylogenetic group. The corresponding avirulence factor in the PVY genome is the NIb cistron (i.e., RNA-dependent RNA polymerase). Here, we describe a new source of potyvirus resistance in the Guatemalan accession <i>C. annuum</i> cv. PM949. PM949 is resistant to members of at least three potyvirus species, a subset of those controlled by <i>Pvr4</i>. The F<sub>1</sub> progeny between PM949 and the susceptible cultivar Yolo Wonder was susceptible to PVY, indicating that the resistance is recessive. The segregation ratio between resistant and susceptible plants observed in the F<sub>2</sub> progeny matched preferably with resistance being determined by two unlinked recessive genes independently conferring resistance to PVY. Inoculations by grafting resulted in the selection of PVY mutants breaking PM949 resistance and, less efficiently, <i>Pvr4</i>–mediated resistance. The codon substitution E<sub>472</sub>K in the NIb cistron of PVY, which was shown previously to be sufficient to break <i>Pvr4</i> resistance, was also sufficient to break PM949 resistance, a rare example of cross-pathogenicity effect. In contrast, the other selected NIb mutants showed specific infectivity in PM949 or <i>Pvr4</i> plants. Comparison of <i>Pvr4</i> and PM949 resistance, which share the same target in PVY, provides interesting insights into the determinants of resistance durability.https://www.mdpi.com/1999-4915/15/5/1081plant resistanceresistance durability<i>Potyvirus</i><i>Capsicum annuum</i>cross-pathogenicityvirulence determinant |
| spellingShingle | Benoît Moury Thierry Michon Vincent Simon Alain Palloix A Single Nonsynonymous Substitution in the RNA-Dependent RNA Polymerase of <i>Potato virus Y</i> Allows the Simultaneous Breakdown of Two Different Forms of Antiviral Resistance in <i>Capsicum annuum</i> Viruses plant resistance resistance durability <i>Potyvirus</i> <i>Capsicum annuum</i> cross-pathogenicity virulence determinant |
| title | A Single Nonsynonymous Substitution in the RNA-Dependent RNA Polymerase of <i>Potato virus Y</i> Allows the Simultaneous Breakdown of Two Different Forms of Antiviral Resistance in <i>Capsicum annuum</i> |
| title_full | A Single Nonsynonymous Substitution in the RNA-Dependent RNA Polymerase of <i>Potato virus Y</i> Allows the Simultaneous Breakdown of Two Different Forms of Antiviral Resistance in <i>Capsicum annuum</i> |
| title_fullStr | A Single Nonsynonymous Substitution in the RNA-Dependent RNA Polymerase of <i>Potato virus Y</i> Allows the Simultaneous Breakdown of Two Different Forms of Antiviral Resistance in <i>Capsicum annuum</i> |
| title_full_unstemmed | A Single Nonsynonymous Substitution in the RNA-Dependent RNA Polymerase of <i>Potato virus Y</i> Allows the Simultaneous Breakdown of Two Different Forms of Antiviral Resistance in <i>Capsicum annuum</i> |
| title_short | A Single Nonsynonymous Substitution in the RNA-Dependent RNA Polymerase of <i>Potato virus Y</i> Allows the Simultaneous Breakdown of Two Different Forms of Antiviral Resistance in <i>Capsicum annuum</i> |
| title_sort | single nonsynonymous substitution in the rna dependent rna polymerase of i potato virus y i allows the simultaneous breakdown of two different forms of antiviral resistance in i capsicum annuum i |
| topic | plant resistance resistance durability <i>Potyvirus</i> <i>Capsicum annuum</i> cross-pathogenicity virulence determinant |
| url | https://www.mdpi.com/1999-4915/15/5/1081 |
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