Integration of molecular biology tools for identifying promoters and genes abundantly expressed in flowers of <it>Oncidium </it>Gower Ramsey
<p>Abstract</p> <p>Background</p> <p>Orchids comprise one of the largest families of flowering plants and generate commercially important flowers. However, model plants, such as <it>Arabidopsis thaliana </it>do not contain all plant genes, and agronomic and...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2011-04-01
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| Series: | BMC Plant Biology |
| Online Access: | http://www.biomedcentral.com/1471-2229/11/60 |
| _version_ | 1818641674439491584 |
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| author | Tung Shu-Yun Chou Shu-Jen Shen Shu-Chen Liu Nien-Tze Wu Fu-Hui Liao De-Chih Hsu Chen-Tran Yang Chang-Hsien Chan Ming-Tsair Lin Choun-Sea |
| author_facet | Tung Shu-Yun Chou Shu-Jen Shen Shu-Chen Liu Nien-Tze Wu Fu-Hui Liao De-Chih Hsu Chen-Tran Yang Chang-Hsien Chan Ming-Tsair Lin Choun-Sea |
| author_sort | Tung Shu-Yun |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Orchids comprise one of the largest families of flowering plants and generate commercially important flowers. However, model plants, such as <it>Arabidopsis thaliana </it>do not contain all plant genes, and agronomic and horticulturally important genera and species must be individually studied.</p> <p>Results</p> <p>Several molecular biology tools were used to isolate flower-specific gene promoters from <it>Oncidium </it>'Gower Ramsey' (<it>Onc</it>. GR). A cDNA library of reproductive tissues was used to construct a microarray in order to compare gene expression in flowers and leaves. Five genes were highly expressed in flower tissues, and the subcellular locations of the corresponding proteins were identified using lip transient transformation with fluorescent protein-fusion constructs. BAC clones of the 5 genes, together with 7 previously published flower- and reproductive growth-specific genes in <it>Onc</it>. GR, were identified for cloning of their promoter regions. Interestingly, 3 of the 5 novel flower-abundant genes were putative trypsin inhibitor (<it>TI</it>) genes (<it>OnTI1</it>, <it>OnTI2 </it>and <it>OnTI3</it>), which were tandemly duplicated in the same BAC clone. Their promoters were identified using transient GUS reporter gene transformation and stable <it>A. thaliana </it>transformation analyses.</p> <p>Conclusions</p> <p>By combining cDNA microarray, BAC library, and bombardment assay techniques, we successfully identified flower-directed orchid genes and promoters.</p> |
| first_indexed | 2024-12-16T23:30:55Z |
| format | Article |
| id | doaj.art-07136c9e6ffb4caaa0dde210f5e8b683 |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-12-16T23:30:55Z |
| publishDate | 2011-04-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-07136c9e6ffb4caaa0dde210f5e8b6832022-12-21T22:11:52ZengBMCBMC Plant Biology1471-22292011-04-011116010.1186/1471-2229-11-60Integration of molecular biology tools for identifying promoters and genes abundantly expressed in flowers of <it>Oncidium </it>Gower RamseyTung Shu-YunChou Shu-JenShen Shu-ChenLiu Nien-TzeWu Fu-HuiLiao De-ChihHsu Chen-TranYang Chang-HsienChan Ming-TsairLin Choun-Sea<p>Abstract</p> <p>Background</p> <p>Orchids comprise one of the largest families of flowering plants and generate commercially important flowers. However, model plants, such as <it>Arabidopsis thaliana </it>do not contain all plant genes, and agronomic and horticulturally important genera and species must be individually studied.</p> <p>Results</p> <p>Several molecular biology tools were used to isolate flower-specific gene promoters from <it>Oncidium </it>'Gower Ramsey' (<it>Onc</it>. GR). A cDNA library of reproductive tissues was used to construct a microarray in order to compare gene expression in flowers and leaves. Five genes were highly expressed in flower tissues, and the subcellular locations of the corresponding proteins were identified using lip transient transformation with fluorescent protein-fusion constructs. BAC clones of the 5 genes, together with 7 previously published flower- and reproductive growth-specific genes in <it>Onc</it>. GR, were identified for cloning of their promoter regions. Interestingly, 3 of the 5 novel flower-abundant genes were putative trypsin inhibitor (<it>TI</it>) genes (<it>OnTI1</it>, <it>OnTI2 </it>and <it>OnTI3</it>), which were tandemly duplicated in the same BAC clone. Their promoters were identified using transient GUS reporter gene transformation and stable <it>A. thaliana </it>transformation analyses.</p> <p>Conclusions</p> <p>By combining cDNA microarray, BAC library, and bombardment assay techniques, we successfully identified flower-directed orchid genes and promoters.</p>http://www.biomedcentral.com/1471-2229/11/60 |
| spellingShingle | Tung Shu-Yun Chou Shu-Jen Shen Shu-Chen Liu Nien-Tze Wu Fu-Hui Liao De-Chih Hsu Chen-Tran Yang Chang-Hsien Chan Ming-Tsair Lin Choun-Sea Integration of molecular biology tools for identifying promoters and genes abundantly expressed in flowers of <it>Oncidium </it>Gower Ramsey BMC Plant Biology |
| title | Integration of molecular biology tools for identifying promoters and genes abundantly expressed in flowers of <it>Oncidium </it>Gower Ramsey |
| title_full | Integration of molecular biology tools for identifying promoters and genes abundantly expressed in flowers of <it>Oncidium </it>Gower Ramsey |
| title_fullStr | Integration of molecular biology tools for identifying promoters and genes abundantly expressed in flowers of <it>Oncidium </it>Gower Ramsey |
| title_full_unstemmed | Integration of molecular biology tools for identifying promoters and genes abundantly expressed in flowers of <it>Oncidium </it>Gower Ramsey |
| title_short | Integration of molecular biology tools for identifying promoters and genes abundantly expressed in flowers of <it>Oncidium </it>Gower Ramsey |
| title_sort | integration of molecular biology tools for identifying promoters and genes abundantly expressed in flowers of it oncidium it gower ramsey |
| url | http://www.biomedcentral.com/1471-2229/11/60 |
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