High Antibiotic Resistance of <i>Helicobacter pylori</i> and Its Associated Novel Gene Mutations among the Mongolian Population
Mongolia has a high prevalence of <i>Helicobacter pylori</i> infection and the second highest incidence of gastric cancer worldwide. Thus, investigating the prevalence of antibiotic resistance and its underlying genetic mechanism is necessary. We isolated 361 <i>H. pylori</i>...
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MDPI AG
2020-07-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/8/7/1062 |
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| author | Dashdorj Azzaya Boldbaatar Gantuya Khasag Oyuntsetseg Duger Davaadorj Takashi Matsumoto Junko Akada Yoshio Yamaoka |
| author_facet | Dashdorj Azzaya Boldbaatar Gantuya Khasag Oyuntsetseg Duger Davaadorj Takashi Matsumoto Junko Akada Yoshio Yamaoka |
| author_sort | Dashdorj Azzaya |
| collection | DOAJ |
| description | Mongolia has a high prevalence of <i>Helicobacter pylori</i> infection and the second highest incidence of gastric cancer worldwide. Thus, investigating the prevalence of antibiotic resistance and its underlying genetic mechanism is necessary. We isolated 361 <i>H. pylori</i> strains throughout Mongolia. Agar dilution assays were used to determine the minimum inhibitory concentrations of five antibiotics; amoxicillin, clarithromycin, metronidazole, levofloxacin, and minocycline. The genetic determinants of antibiotic resistance were identified with next-generation sequencing (NGS) and the CLC Genomics Workbench. The resistance to metronidazole, levofloxacin, clarithromycin, amoxicillin, and minocycline was 78.7%, 41.3%, 29.9%, 11.9% and 0.28%, respectively. Multidrug resistance was identified in 51.3% of the isolates investigated which were further delineated into 9 antimicrobial resistance profiles. A number of known antibiotic resistance mutations were identified including <i>rdxA</i>, <i>frxA</i> (missense, frameshift), <i>gyrA</i> (N87K, A88P, D91G/N/Y), 23S rRNA (A2143G), <i>pbp1A</i> (N562Y), and 16S rRNA (A928C). Furthermore, we detected previously unreported mutations in <i>pbp1A</i> (L610*) and the 23S rRNA gene (A1410G, C1707T, A2167G, C2248T, and C2922T). The degree of antibiotic resistance was high, indicating the insufficiency of standard triple therapy in Mongolia. |
| first_indexed | 2024-03-10T18:26:19Z |
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| language | English |
| last_indexed | 2024-03-10T18:26:19Z |
| publishDate | 2020-07-01 |
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| series | Microorganisms |
| spelling | doaj.art-071e104c63c445d885eadf248e27ca772023-11-20T06:59:31ZengMDPI AGMicroorganisms2076-26072020-07-0187106210.3390/microorganisms8071062High Antibiotic Resistance of <i>Helicobacter pylori</i> and Its Associated Novel Gene Mutations among the Mongolian PopulationDashdorj Azzaya0Boldbaatar Gantuya1Khasag Oyuntsetseg2Duger Davaadorj3Takashi Matsumoto4Junko Akada5Yoshio Yamaoka6Department of Environmental and Preventive Medicine, Faculty of Medicine, Oita University, Yufu City, Oita 879-5593, JapanDepartment of Gastroenterology, Mongolian National University of Medical Sciences, Ulaanbaatar 14210, MongoliaDepartment of Gastroenterology, Mongolian National University of Medical Sciences, Ulaanbaatar 14210, MongoliaDepartment of Gastroenterology, Mongolian National University of Medical Sciences, Ulaanbaatar 14210, MongoliaDepartment of Environmental and Preventive Medicine, Faculty of Medicine, Oita University, Yufu City, Oita 879-5593, JapanDepartment of Environmental and Preventive Medicine, Faculty of Medicine, Oita University, Yufu City, Oita 879-5593, JapanDepartment of Environmental and Preventive Medicine, Faculty of Medicine, Oita University, Yufu City, Oita 879-5593, JapanMongolia has a high prevalence of <i>Helicobacter pylori</i> infection and the second highest incidence of gastric cancer worldwide. Thus, investigating the prevalence of antibiotic resistance and its underlying genetic mechanism is necessary. We isolated 361 <i>H. pylori</i> strains throughout Mongolia. Agar dilution assays were used to determine the minimum inhibitory concentrations of five antibiotics; amoxicillin, clarithromycin, metronidazole, levofloxacin, and minocycline. The genetic determinants of antibiotic resistance were identified with next-generation sequencing (NGS) and the CLC Genomics Workbench. The resistance to metronidazole, levofloxacin, clarithromycin, amoxicillin, and minocycline was 78.7%, 41.3%, 29.9%, 11.9% and 0.28%, respectively. Multidrug resistance was identified in 51.3% of the isolates investigated which were further delineated into 9 antimicrobial resistance profiles. A number of known antibiotic resistance mutations were identified including <i>rdxA</i>, <i>frxA</i> (missense, frameshift), <i>gyrA</i> (N87K, A88P, D91G/N/Y), 23S rRNA (A2143G), <i>pbp1A</i> (N562Y), and 16S rRNA (A928C). Furthermore, we detected previously unreported mutations in <i>pbp1A</i> (L610*) and the 23S rRNA gene (A1410G, C1707T, A2167G, C2248T, and C2922T). The degree of antibiotic resistance was high, indicating the insufficiency of standard triple therapy in Mongolia.https://www.mdpi.com/2076-2607/8/7/1062<i>Helicobacter pylori</i>antibiotic resistancewhole genome sequencingresistance mutationnext-generation sequencingMongolia |
| spellingShingle | Dashdorj Azzaya Boldbaatar Gantuya Khasag Oyuntsetseg Duger Davaadorj Takashi Matsumoto Junko Akada Yoshio Yamaoka High Antibiotic Resistance of <i>Helicobacter pylori</i> and Its Associated Novel Gene Mutations among the Mongolian Population Microorganisms <i>Helicobacter pylori</i> antibiotic resistance whole genome sequencing resistance mutation next-generation sequencing Mongolia |
| title | High Antibiotic Resistance of <i>Helicobacter pylori</i> and Its Associated Novel Gene Mutations among the Mongolian Population |
| title_full | High Antibiotic Resistance of <i>Helicobacter pylori</i> and Its Associated Novel Gene Mutations among the Mongolian Population |
| title_fullStr | High Antibiotic Resistance of <i>Helicobacter pylori</i> and Its Associated Novel Gene Mutations among the Mongolian Population |
| title_full_unstemmed | High Antibiotic Resistance of <i>Helicobacter pylori</i> and Its Associated Novel Gene Mutations among the Mongolian Population |
| title_short | High Antibiotic Resistance of <i>Helicobacter pylori</i> and Its Associated Novel Gene Mutations among the Mongolian Population |
| title_sort | high antibiotic resistance of i helicobacter pylori i and its associated novel gene mutations among the mongolian population |
| topic | <i>Helicobacter pylori</i> antibiotic resistance whole genome sequencing resistance mutation next-generation sequencing Mongolia |
| url | https://www.mdpi.com/2076-2607/8/7/1062 |
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