Effect of the medium composition on formation of amylase by Bacillus sp
Studies on the alpha -amylase synthesis was carried out with a moderately thermophilic, facultatively anaerobic Bacillus sp, isolated from soil samples. The cells were cultivated in a complex medium containing soluble starch or maltose as carbon source. The levels of the alpha -amylaseactivity detec...
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| Format: | Article |
| Language: | English |
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Instituto de Tecnologia do Paraná (Tecpar)
2003-01-01
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| Series: | Brazilian Archives of Biology and Technology |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000100018 |
| _version_ | 1818153707560239104 |
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| author | Eliana de Oliveira. Santos Meire Lelis Leal Martins |
| author_facet | Eliana de Oliveira. Santos Meire Lelis Leal Martins |
| author_sort | Eliana de Oliveira. Santos |
| collection | DOAJ |
| description | Studies on the alpha -amylase synthesis was carried out with a moderately thermophilic, facultatively anaerobic Bacillus sp, isolated from soil samples. The cells were cultivated in a complex medium containing soluble starch or maltose as carbon source. The levels of the alpha -amylaseactivity detected in culture supernatants varied greatly with the type of carbon source used. Maltose, soluble starch and citrate stimulated alpha -amylaseformation. Addition of exogenous glucose repressed formation of alpha -amylase, demonstrating that a classical glucose effect was operative in this organism. The concentration of yeast extract was found to be important factor in the alpha -amylase synthesis bythe isolate.The activity of the enzyme increased between 2 and 5 g/L yeast extract concentration and then fell very rapidly beyond this point. The best concentration of peptone to alpha-amylase formation was found to be around 10g/L.<br>Estudos sobre a síntese de alfa -amilase foram realizados com uma bactéria termofílica moderada e facultativa anaeróbica, isolada de amostras de solo. As células foram cultivadas em um meio complexo contendo amido solúvel ou maltose como fonte de carbono. Os níveis da atividade de alfa -amilase detectados no sobrenadante da cultura variaram grandemente com o tipo da fonte de carbono utilizada. Amido solúvel, maltose e citrato estimularam a formação de alfa -amilase. A adição de glicose as culturas reprimiu a formação da alfa -amilase, demonstrando que o clássico efeito glicose foi operativo neste organismo. A concentração de extrato de levedura foi um fator importante na formação de alfa -amilase pelo isolado. A atividade da enzima aumentou entre concentrações de 2 a 5 g/L e então caiu muito rapidamente em torno deste ponto. A melhor concentração de peptona para a formação da alfa -amilase foi em torno de 10 g/L. |
| first_indexed | 2024-12-11T14:14:54Z |
| format | Article |
| id | doaj.art-071e3bddc6b3462aaf6bff8bd58a9178 |
| institution | Directory Open Access Journal |
| issn | 1516-8913 1678-4324 |
| language | English |
| last_indexed | 2024-12-11T14:14:54Z |
| publishDate | 2003-01-01 |
| publisher | Instituto de Tecnologia do Paraná (Tecpar) |
| record_format | Article |
| series | Brazilian Archives of Biology and Technology |
| spelling | doaj.art-071e3bddc6b3462aaf6bff8bd58a91782022-12-22T01:03:13ZengInstituto de Tecnologia do Paraná (Tecpar)Brazilian Archives of Biology and Technology1516-89131678-43242003-01-0146112913410.1590/S1516-89132003000100018Effect of the medium composition on formation of amylase by Bacillus spEliana de Oliveira. SantosMeire Lelis Leal MartinsStudies on the alpha -amylase synthesis was carried out with a moderately thermophilic, facultatively anaerobic Bacillus sp, isolated from soil samples. The cells were cultivated in a complex medium containing soluble starch or maltose as carbon source. The levels of the alpha -amylaseactivity detected in culture supernatants varied greatly with the type of carbon source used. Maltose, soluble starch and citrate stimulated alpha -amylaseformation. Addition of exogenous glucose repressed formation of alpha -amylase, demonstrating that a classical glucose effect was operative in this organism. The concentration of yeast extract was found to be important factor in the alpha -amylase synthesis bythe isolate.The activity of the enzyme increased between 2 and 5 g/L yeast extract concentration and then fell very rapidly beyond this point. The best concentration of peptone to alpha-amylase formation was found to be around 10g/L.<br>Estudos sobre a síntese de alfa -amilase foram realizados com uma bactéria termofílica moderada e facultativa anaeróbica, isolada de amostras de solo. As células foram cultivadas em um meio complexo contendo amido solúvel ou maltose como fonte de carbono. Os níveis da atividade de alfa -amilase detectados no sobrenadante da cultura variaram grandemente com o tipo da fonte de carbono utilizada. Amido solúvel, maltose e citrato estimularam a formação de alfa -amilase. A adição de glicose as culturas reprimiu a formação da alfa -amilase, demonstrando que o clássico efeito glicose foi operativo neste organismo. A concentração de extrato de levedura foi um fator importante na formação de alfa -amilase pelo isolado. A atividade da enzima aumentou entre concentrações de 2 a 5 g/L e então caiu muito rapidamente em torno deste ponto. A melhor concentração de peptona para a formação da alfa -amilase foi em torno de 10 g/L.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000100018alpha-amylasethermophilic bacteriumBacillus sp |
| spellingShingle | Eliana de Oliveira. Santos Meire Lelis Leal Martins Effect of the medium composition on formation of amylase by Bacillus sp Brazilian Archives of Biology and Technology alpha-amylase thermophilic bacterium Bacillus sp |
| title | Effect of the medium composition on formation of amylase by Bacillus sp |
| title_full | Effect of the medium composition on formation of amylase by Bacillus sp |
| title_fullStr | Effect of the medium composition on formation of amylase by Bacillus sp |
| title_full_unstemmed | Effect of the medium composition on formation of amylase by Bacillus sp |
| title_short | Effect of the medium composition on formation of amylase by Bacillus sp |
| title_sort | effect of the medium composition on formation of amylase by bacillus sp |
| topic | alpha-amylase thermophilic bacterium Bacillus sp |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000100018 |
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