Magnetic Lateral Flow Immunoassay for Small Extracellular Vesicles Quantification: Application to Colorectal Cancer Biomarker Detection
Colorectal cancer (CRC) is the third leading cause of cancer death and the fourth most common cancer in the world. Colonoscopy is the most sensitive test used for detection of CRC; however, their procedure is invasive and expensive for population mass screening. Currently, the fecal occult blood tes...
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MDPI AG
2021-05-01
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Online Access: | https://www.mdpi.com/1424-8220/21/11/3756 |
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author | Amanda Moyano Esther Serrano-Pertierra José María Duque Virginia Ramos Estefanía Teruel-Barandiarán María Teresa Fernández-Sánchez María Salvador José Carlos Martínez-García Luis Sánchez Luis García-Flórez Montserrat Rivas María del Carmen Blanco-López |
author_facet | Amanda Moyano Esther Serrano-Pertierra José María Duque Virginia Ramos Estefanía Teruel-Barandiarán María Teresa Fernández-Sánchez María Salvador José Carlos Martínez-García Luis Sánchez Luis García-Flórez Montserrat Rivas María del Carmen Blanco-López |
author_sort | Amanda Moyano |
collection | DOAJ |
description | Colorectal cancer (CRC) is the third leading cause of cancer death and the fourth most common cancer in the world. Colonoscopy is the most sensitive test used for detection of CRC; however, their procedure is invasive and expensive for population mass screening. Currently, the fecal occult blood test has been widely used as a screening tool for CRC but displays low specificity. The lack of rapid and simple methods for mass screening makes the early diagnosis and therapy monitoring difficult. Extracellular vesicles (EVs) have emerged as a novel source of biomarkers due to their contents in proteins and miRNAs. Their detection would not require invasive techniques and could be considered as a liquid biopsy. Specifically, it has been demonstrated that the amount of CD147 expressed in circulating EVs is significant higher for CRC cell lines than for normal colon fibroblast cell lines. Moreover, CD147-containing EVs have been used as a biomarker to monitor response to therapy in patients with CRC. Therefore, this antigen could be used as a non-invasive biomarker for the detection and monitoring of CRC in combination with a Point-of-Care platform as, for example, Lateral Flow Immunoassays (LFIAs). Here, we propose the development of a quantitative lateral flow immunoassay test based on the use of magnetic nanoparticles as labels coupled to inductive sensor for the non-invasive detection of CRC by CD147-positive EVs. The results obtained for quantification of CD147 antigen embedded in EVs isolated from plasma sample have demonstrated that this device could be used as a Point-of-Care tool for CRC screening or therapy monitoring thanks to its rapid response and easy operation. |
first_indexed | 2024-03-10T10:55:15Z |
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issn | 1424-8220 |
language | English |
last_indexed | 2024-03-10T10:55:15Z |
publishDate | 2021-05-01 |
publisher | MDPI AG |
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series | Sensors |
spelling | doaj.art-071f8138fb5a4f589bc4b768dae184102023-11-21T21:52:11ZengMDPI AGSensors1424-82202021-05-012111375610.3390/s21113756Magnetic Lateral Flow Immunoassay for Small Extracellular Vesicles Quantification: Application to Colorectal Cancer Biomarker DetectionAmanda Moyano0Esther Serrano-Pertierra1José María Duque2Virginia Ramos3Estefanía Teruel-Barandiarán4María Teresa Fernández-Sánchez5María Salvador6José Carlos Martínez-García7Luis Sánchez8Luis García-Flórez9Montserrat Rivas10María del Carmen Blanco-López11Department of Physical and Analytical Chemistry & Institute of Biotechnology of Asturias, University of Oviedo, c/Julián Clavería 8, 33006 Oviedo, SpainDepartment of Physical and Analytical Chemistry & Institute of Biotechnology of Asturias, University of Oviedo, c/Julián Clavería 8, 33006 Oviedo, SpainHospital Universitario San Agustín, 33401 Avilés, SpainHospital Universitario San Agustín, 33401 Avilés, SpainDepartment of Physical and Analytical Chemistry & Institute of Biotechnology of Asturias, University of Oviedo, c/Julián Clavería 8, 33006 Oviedo, SpainDepartment of Biochemistry and Molecular Biology & Institute of Biotechnology of Asturias, University of Oviedo, 33006 Oviedo, SpainDepartment of Physics & IUTA, University of Oviedo, Campus de Viesques, 33204 Gijón, SpainDepartment of Physics & IUTA, University of Oviedo, Campus de Viesques, 33204 Gijón, SpainHospital Universitario San Agustín, 33401 Avilés, SpainHospital Universitario Central de Asturias (HUCA), 33011 Oviedo, SpainDepartment of Physics & IUTA, University of Oviedo, Campus de Viesques, 33204 Gijón, SpainDepartment of Physical and Analytical Chemistry & Institute of Biotechnology of Asturias, University of Oviedo, c/Julián Clavería 8, 33006 Oviedo, SpainColorectal cancer (CRC) is the third leading cause of cancer death and the fourth most common cancer in the world. Colonoscopy is the most sensitive test used for detection of CRC; however, their procedure is invasive and expensive for population mass screening. Currently, the fecal occult blood test has been widely used as a screening tool for CRC but displays low specificity. The lack of rapid and simple methods for mass screening makes the early diagnosis and therapy monitoring difficult. Extracellular vesicles (EVs) have emerged as a novel source of biomarkers due to their contents in proteins and miRNAs. Their detection would not require invasive techniques and could be considered as a liquid biopsy. Specifically, it has been demonstrated that the amount of CD147 expressed in circulating EVs is significant higher for CRC cell lines than for normal colon fibroblast cell lines. Moreover, CD147-containing EVs have been used as a biomarker to monitor response to therapy in patients with CRC. Therefore, this antigen could be used as a non-invasive biomarker for the detection and monitoring of CRC in combination with a Point-of-Care platform as, for example, Lateral Flow Immunoassays (LFIAs). Here, we propose the development of a quantitative lateral flow immunoassay test based on the use of magnetic nanoparticles as labels coupled to inductive sensor for the non-invasive detection of CRC by CD147-positive EVs. The results obtained for quantification of CD147 antigen embedded in EVs isolated from plasma sample have demonstrated that this device could be used as a Point-of-Care tool for CRC screening or therapy monitoring thanks to its rapid response and easy operation.https://www.mdpi.com/1424-8220/21/11/3756colorectal cancerblood biomarkersextracellular vesiclesCD147 antigenmagnetic lateral flow immunoassayinductive sensors |
spellingShingle | Amanda Moyano Esther Serrano-Pertierra José María Duque Virginia Ramos Estefanía Teruel-Barandiarán María Teresa Fernández-Sánchez María Salvador José Carlos Martínez-García Luis Sánchez Luis García-Flórez Montserrat Rivas María del Carmen Blanco-López Magnetic Lateral Flow Immunoassay for Small Extracellular Vesicles Quantification: Application to Colorectal Cancer Biomarker Detection Sensors colorectal cancer blood biomarkers extracellular vesicles CD147 antigen magnetic lateral flow immunoassay inductive sensors |
title | Magnetic Lateral Flow Immunoassay for Small Extracellular Vesicles Quantification: Application to Colorectal Cancer Biomarker Detection |
title_full | Magnetic Lateral Flow Immunoassay for Small Extracellular Vesicles Quantification: Application to Colorectal Cancer Biomarker Detection |
title_fullStr | Magnetic Lateral Flow Immunoassay for Small Extracellular Vesicles Quantification: Application to Colorectal Cancer Biomarker Detection |
title_full_unstemmed | Magnetic Lateral Flow Immunoassay for Small Extracellular Vesicles Quantification: Application to Colorectal Cancer Biomarker Detection |
title_short | Magnetic Lateral Flow Immunoassay for Small Extracellular Vesicles Quantification: Application to Colorectal Cancer Biomarker Detection |
title_sort | magnetic lateral flow immunoassay for small extracellular vesicles quantification application to colorectal cancer biomarker detection |
topic | colorectal cancer blood biomarkers extracellular vesicles CD147 antigen magnetic lateral flow immunoassay inductive sensors |
url | https://www.mdpi.com/1424-8220/21/11/3756 |
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