Magnetic Lateral Flow Immunoassay for Small Extracellular Vesicles Quantification: Application to Colorectal Cancer Biomarker Detection

Colorectal cancer (CRC) is the third leading cause of cancer death and the fourth most common cancer in the world. Colonoscopy is the most sensitive test used for detection of CRC; however, their procedure is invasive and expensive for population mass screening. Currently, the fecal occult blood tes...

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Main Authors: Amanda Moyano, Esther Serrano-Pertierra, José María Duque, Virginia Ramos, Estefanía Teruel-Barandiarán, María Teresa Fernández-Sánchez, María Salvador, José Carlos Martínez-García, Luis Sánchez, Luis García-Flórez, Montserrat Rivas, María del Carmen Blanco-López
Format: Article
Language:English
Published: MDPI AG 2021-05-01
Series:Sensors
Subjects:
Online Access:https://www.mdpi.com/1424-8220/21/11/3756
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author Amanda Moyano
Esther Serrano-Pertierra
José María Duque
Virginia Ramos
Estefanía Teruel-Barandiarán
María Teresa Fernández-Sánchez
María Salvador
José Carlos Martínez-García
Luis Sánchez
Luis García-Flórez
Montserrat Rivas
María del Carmen Blanco-López
author_facet Amanda Moyano
Esther Serrano-Pertierra
José María Duque
Virginia Ramos
Estefanía Teruel-Barandiarán
María Teresa Fernández-Sánchez
María Salvador
José Carlos Martínez-García
Luis Sánchez
Luis García-Flórez
Montserrat Rivas
María del Carmen Blanco-López
author_sort Amanda Moyano
collection DOAJ
description Colorectal cancer (CRC) is the third leading cause of cancer death and the fourth most common cancer in the world. Colonoscopy is the most sensitive test used for detection of CRC; however, their procedure is invasive and expensive for population mass screening. Currently, the fecal occult blood test has been widely used as a screening tool for CRC but displays low specificity. The lack of rapid and simple methods for mass screening makes the early diagnosis and therapy monitoring difficult. Extracellular vesicles (EVs) have emerged as a novel source of biomarkers due to their contents in proteins and miRNAs. Their detection would not require invasive techniques and could be considered as a liquid biopsy. Specifically, it has been demonstrated that the amount of CD147 expressed in circulating EVs is significant higher for CRC cell lines than for normal colon fibroblast cell lines. Moreover, CD147-containing EVs have been used as a biomarker to monitor response to therapy in patients with CRC. Therefore, this antigen could be used as a non-invasive biomarker for the detection and monitoring of CRC in combination with a Point-of-Care platform as, for example, Lateral Flow Immunoassays (LFIAs). Here, we propose the development of a quantitative lateral flow immunoassay test based on the use of magnetic nanoparticles as labels coupled to inductive sensor for the non-invasive detection of CRC by CD147-positive EVs. The results obtained for quantification of CD147 antigen embedded in EVs isolated from plasma sample have demonstrated that this device could be used as a Point-of-Care tool for CRC screening or therapy monitoring thanks to its rapid response and easy operation.
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spelling doaj.art-071f8138fb5a4f589bc4b768dae184102023-11-21T21:52:11ZengMDPI AGSensors1424-82202021-05-012111375610.3390/s21113756Magnetic Lateral Flow Immunoassay for Small Extracellular Vesicles Quantification: Application to Colorectal Cancer Biomarker DetectionAmanda Moyano0Esther Serrano-Pertierra1José María Duque2Virginia Ramos3Estefanía Teruel-Barandiarán4María Teresa Fernández-Sánchez5María Salvador6José Carlos Martínez-García7Luis Sánchez8Luis García-Flórez9Montserrat Rivas10María del Carmen Blanco-López11Department of Physical and Analytical Chemistry & Institute of Biotechnology of Asturias, University of Oviedo, c/Julián Clavería 8, 33006 Oviedo, SpainDepartment of Physical and Analytical Chemistry & Institute of Biotechnology of Asturias, University of Oviedo, c/Julián Clavería 8, 33006 Oviedo, SpainHospital Universitario San Agustín, 33401 Avilés, SpainHospital Universitario San Agustín, 33401 Avilés, SpainDepartment of Physical and Analytical Chemistry & Institute of Biotechnology of Asturias, University of Oviedo, c/Julián Clavería 8, 33006 Oviedo, SpainDepartment of Biochemistry and Molecular Biology & Institute of Biotechnology of Asturias, University of Oviedo, 33006 Oviedo, SpainDepartment of Physics & IUTA, University of Oviedo, Campus de Viesques, 33204 Gijón, SpainDepartment of Physics & IUTA, University of Oviedo, Campus de Viesques, 33204 Gijón, SpainHospital Universitario San Agustín, 33401 Avilés, SpainHospital Universitario Central de Asturias (HUCA), 33011 Oviedo, SpainDepartment of Physics & IUTA, University of Oviedo, Campus de Viesques, 33204 Gijón, SpainDepartment of Physical and Analytical Chemistry & Institute of Biotechnology of Asturias, University of Oviedo, c/Julián Clavería 8, 33006 Oviedo, SpainColorectal cancer (CRC) is the third leading cause of cancer death and the fourth most common cancer in the world. Colonoscopy is the most sensitive test used for detection of CRC; however, their procedure is invasive and expensive for population mass screening. Currently, the fecal occult blood test has been widely used as a screening tool for CRC but displays low specificity. The lack of rapid and simple methods for mass screening makes the early diagnosis and therapy monitoring difficult. Extracellular vesicles (EVs) have emerged as a novel source of biomarkers due to their contents in proteins and miRNAs. Their detection would not require invasive techniques and could be considered as a liquid biopsy. Specifically, it has been demonstrated that the amount of CD147 expressed in circulating EVs is significant higher for CRC cell lines than for normal colon fibroblast cell lines. Moreover, CD147-containing EVs have been used as a biomarker to monitor response to therapy in patients with CRC. Therefore, this antigen could be used as a non-invasive biomarker for the detection and monitoring of CRC in combination with a Point-of-Care platform as, for example, Lateral Flow Immunoassays (LFIAs). Here, we propose the development of a quantitative lateral flow immunoassay test based on the use of magnetic nanoparticles as labels coupled to inductive sensor for the non-invasive detection of CRC by CD147-positive EVs. The results obtained for quantification of CD147 antigen embedded in EVs isolated from plasma sample have demonstrated that this device could be used as a Point-of-Care tool for CRC screening or therapy monitoring thanks to its rapid response and easy operation.https://www.mdpi.com/1424-8220/21/11/3756colorectal cancerblood biomarkersextracellular vesiclesCD147 antigenmagnetic lateral flow immunoassayinductive sensors
spellingShingle Amanda Moyano
Esther Serrano-Pertierra
José María Duque
Virginia Ramos
Estefanía Teruel-Barandiarán
María Teresa Fernández-Sánchez
María Salvador
José Carlos Martínez-García
Luis Sánchez
Luis García-Flórez
Montserrat Rivas
María del Carmen Blanco-López
Magnetic Lateral Flow Immunoassay for Small Extracellular Vesicles Quantification: Application to Colorectal Cancer Biomarker Detection
Sensors
colorectal cancer
blood biomarkers
extracellular vesicles
CD147 antigen
magnetic lateral flow immunoassay
inductive sensors
title Magnetic Lateral Flow Immunoassay for Small Extracellular Vesicles Quantification: Application to Colorectal Cancer Biomarker Detection
title_full Magnetic Lateral Flow Immunoassay for Small Extracellular Vesicles Quantification: Application to Colorectal Cancer Biomarker Detection
title_fullStr Magnetic Lateral Flow Immunoassay for Small Extracellular Vesicles Quantification: Application to Colorectal Cancer Biomarker Detection
title_full_unstemmed Magnetic Lateral Flow Immunoassay for Small Extracellular Vesicles Quantification: Application to Colorectal Cancer Biomarker Detection
title_short Magnetic Lateral Flow Immunoassay for Small Extracellular Vesicles Quantification: Application to Colorectal Cancer Biomarker Detection
title_sort magnetic lateral flow immunoassay for small extracellular vesicles quantification application to colorectal cancer biomarker detection
topic colorectal cancer
blood biomarkers
extracellular vesicles
CD147 antigen
magnetic lateral flow immunoassay
inductive sensors
url https://www.mdpi.com/1424-8220/21/11/3756
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