A Possible Role of VPS13B in the Formation of Golgi-Lipid Droplet Contacts Associating with the ER
While the physical interactions between the Golgi apparatus (Golgi) and lipid droplets (LDs) have been suggested through system-level imaging, the Golgi-LD membrane contact sites (MCSs) remain largely uncharacterized. Here, we show evidence to support the existence of Golgi-LD MCSs in HEK293 cells....
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
SAGE Publishing
2023-09-01
|
| Series: | Contact |
| Online Access: | https://doi.org/10.1177/25152564231195718 |
| _version_ | 1797688204362514432 |
|---|---|
| author | Yuanjiao Du Xuewen Hu Weiping Chang Lin Deng Wei-Ke Ji Juan Xiong |
| author_facet | Yuanjiao Du Xuewen Hu Weiping Chang Lin Deng Wei-Ke Ji Juan Xiong |
| author_sort | Yuanjiao Du |
| collection | DOAJ |
| description | While the physical interactions between the Golgi apparatus (Golgi) and lipid droplets (LDs) have been suggested through system-level imaging, the Golgi-LD membrane contact sites (MCSs) remain largely uncharacterized. Here, we show evidence to support the existence of Golgi-LD MCSs in HEK293 cells. We further suggest that vacuolar protein sorting-associated protein 13B (VPS13B) localizes to and promotes the formation of Golgi-LD contacts upon oleic acid (OA) stimulation using 3D high-resolution microscopy. Depletion of VPS13B moderately affects the formation of Golgi-LD contacts upon OA treatment in addition to the fragmentation of the Golgi. Although cellular functions of VPS13B-mediated contacts are still elusive, these findings may provide a new insight into related diseases caused by loss-of-function mutations of VPS13B. |
| first_indexed | 2024-03-12T01:28:55Z |
| format | Article |
| id | doaj.art-07209d15abbd4808a77c62b191b7b2ed |
| institution | Directory Open Access Journal |
| issn | 2515-2564 |
| language | English |
| last_indexed | 2024-03-12T01:28:55Z |
| publishDate | 2023-09-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Contact |
| spelling | doaj.art-07209d15abbd4808a77c62b191b7b2ed2023-09-12T09:03:23ZengSAGE PublishingContact2515-25642023-09-01610.1177/25152564231195718A Possible Role of VPS13B in the Formation of Golgi-Lipid Droplet Contacts Associating with the ERYuanjiao Du0Xuewen Hu1Weiping Chang2Lin Deng3Wei-Ke Ji4Juan Xiong5 Department of Biochemistry and Molecular Biology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China Department of Biochemistry and Molecular Biology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China , Shenzhen, China , Shenzhen, China Cell Architecture Research Center; Huazhong University of Science and Technology, Wuhan, Hubei, China Department of Anesthesiology, Hubei Key Laboratory of Geriatric Anesthesia and Perioperative Brain Health, and Wuhan Clinical Research Center for Geriatric Anesthesia, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China While the physical interactions between the Golgi apparatus (Golgi) and lipid droplets (LDs) have been suggested through system-level imaging, the Golgi-LD membrane contact sites (MCSs) remain largely uncharacterized. Here, we show evidence to support the existence of Golgi-LD MCSs in HEK293 cells. We further suggest that vacuolar protein sorting-associated protein 13B (VPS13B) localizes to and promotes the formation of Golgi-LD contacts upon oleic acid (OA) stimulation using 3D high-resolution microscopy. Depletion of VPS13B moderately affects the formation of Golgi-LD contacts upon OA treatment in addition to the fragmentation of the Golgi. Although cellular functions of VPS13B-mediated contacts are still elusive, these findings may provide a new insight into related diseases caused by loss-of-function mutations of VPS13B.https://doi.org/10.1177/25152564231195718 |
| spellingShingle | Yuanjiao Du Xuewen Hu Weiping Chang Lin Deng Wei-Ke Ji Juan Xiong A Possible Role of VPS13B in the Formation of Golgi-Lipid Droplet Contacts Associating with the ER Contact |
| title | A Possible Role of VPS13B in the Formation of Golgi-Lipid Droplet Contacts Associating with the ER |
| title_full | A Possible Role of VPS13B in the Formation of Golgi-Lipid Droplet Contacts Associating with the ER |
| title_fullStr | A Possible Role of VPS13B in the Formation of Golgi-Lipid Droplet Contacts Associating with the ER |
| title_full_unstemmed | A Possible Role of VPS13B in the Formation of Golgi-Lipid Droplet Contacts Associating with the ER |
| title_short | A Possible Role of VPS13B in the Formation of Golgi-Lipid Droplet Contacts Associating with the ER |
| title_sort | possible role of vps13b in the formation of golgi lipid droplet contacts associating with the er |
| url | https://doi.org/10.1177/25152564231195718 |
| work_keys_str_mv | AT yuanjiaodu apossibleroleofvps13bintheformationofgolgilipiddropletcontactsassociatingwiththeer AT xuewenhu apossibleroleofvps13bintheformationofgolgilipiddropletcontactsassociatingwiththeer AT weipingchang apossibleroleofvps13bintheformationofgolgilipiddropletcontactsassociatingwiththeer AT lindeng apossibleroleofvps13bintheformationofgolgilipiddropletcontactsassociatingwiththeer AT weikeji apossibleroleofvps13bintheformationofgolgilipiddropletcontactsassociatingwiththeer AT juanxiong apossibleroleofvps13bintheformationofgolgilipiddropletcontactsassociatingwiththeer AT yuanjiaodu possibleroleofvps13bintheformationofgolgilipiddropletcontactsassociatingwiththeer AT xuewenhu possibleroleofvps13bintheformationofgolgilipiddropletcontactsassociatingwiththeer AT weipingchang possibleroleofvps13bintheformationofgolgilipiddropletcontactsassociatingwiththeer AT lindeng possibleroleofvps13bintheformationofgolgilipiddropletcontactsassociatingwiththeer AT weikeji possibleroleofvps13bintheformationofgolgilipiddropletcontactsassociatingwiththeer AT juanxiong possibleroleofvps13bintheformationofgolgilipiddropletcontactsassociatingwiththeer |