Chemogenetic activation of astrocytes promotes remyelination and restores cognitive deficits in visceral hypersensitive rats
Summary: Using a well-established chronic visceral hypersensitivity (VH) rat model, we characterized the decrease of myelin basic protein, reduced number of mature oligodendrocytes (OLs), and hypomyelination in the anterior cingulate cortex (ACC). The results of rat gambling test showed impaired dec...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-01-01
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| Series: | iScience |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004222021137 |
| _version_ | 1828057103475933184 |
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| author | Mahadi Hasan Zhuogui Lei Mastura Akter Zafar Iqbal Faeeqa Usaila Aruna Surendran Ramkrishnan Ying Li |
| author_facet | Mahadi Hasan Zhuogui Lei Mastura Akter Zafar Iqbal Faeeqa Usaila Aruna Surendran Ramkrishnan Ying Li |
| author_sort | Mahadi Hasan |
| collection | DOAJ |
| description | Summary: Using a well-established chronic visceral hypersensitivity (VH) rat model, we characterized the decrease of myelin basic protein, reduced number of mature oligodendrocytes (OLs), and hypomyelination in the anterior cingulate cortex (ACC). The results of rat gambling test showed impaired decision-making, and the results of electrophysiological studies showed desynchronization in the ACC to basolateral amygdala (BLA) neural circuitry. Astrocytes release various factors that modulate oligodendrocyte progenitor cell proliferation and myelination. Astrocytic Gq-modulation through expression of hM3Dq facilitated oligodendrocyte progenitor cell proliferation and OL differentiation, and enhanced ACC myelination in VH rats. Activating astrocytic Gq rescued impaired decision-making and desynchronization in ACC-BLA. These data indicate that ACC hypomyelination is an important component of impaired decision-making and network desynchronization in VH. Astrocytic Gq activity plays a significant role in oligodendrocyte myelination and decision-making behavior in VH. Insights from these studies have potential for interventions in myelin-related diseases such as chronic pain-associated cognitive disorders. |
| first_indexed | 2024-04-10T21:06:03Z |
| format | Article |
| id | doaj.art-07250e4819b94a8491e1b89b7fcdd298 |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-04-10T21:06:03Z |
| publishDate | 2023-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-07250e4819b94a8491e1b89b7fcdd2982023-01-22T04:41:25ZengElsevieriScience2589-00422023-01-01261105840Chemogenetic activation of astrocytes promotes remyelination and restores cognitive deficits in visceral hypersensitive ratsMahadi Hasan0Zhuogui Lei1Mastura Akter2Zafar Iqbal3Faeeqa Usaila4Aruna Surendran Ramkrishnan5Ying Li6Department of Neuroscience, City University of Hong Kong, Hong Kong SAR, China; Department of Biomedical Sciences, City University of Hong Kong, Hong Kong SAR, ChinaDepartment of Neuroscience, City University of Hong Kong, Hong Kong SAR, China; Department of Biomedical Sciences, City University of Hong Kong, Hong Kong SAR, China; Centre for Regenerative Medicine and Health, Hong Kong Institute of Science & Innovation, Chinese Academy of Sciences, Hong Kong SAR, ChinaDepartment of Neuroscience, City University of Hong Kong, Hong Kong SAR, China; Department of Biomedical Sciences, City University of Hong Kong, Hong Kong SAR, ChinaDepartment of Neuroscience, City University of Hong Kong, Hong Kong SAR, China; Department of Biomedical Sciences, City University of Hong Kong, Hong Kong SAR, China; Centre for Regenerative Medicine and Health, Hong Kong Institute of Science & Innovation, Chinese Academy of Sciences, Hong Kong SAR, ChinaDepartment of Neuroscience, City University of Hong Kong, Hong Kong SAR, China; Department of Biomedical Sciences, City University of Hong Kong, Hong Kong SAR, ChinaDepartment of Neuroscience, City University of Hong Kong, Hong Kong SAR, China; Department of Biomedical Sciences, City University of Hong Kong, Hong Kong SAR, ChinaDepartment of Neuroscience, City University of Hong Kong, Hong Kong SAR, China; Department of Biomedical Sciences, City University of Hong Kong, Hong Kong SAR, China; Centre for Regenerative Medicine and Health, Hong Kong Institute of Science & Innovation, Chinese Academy of Sciences, Hong Kong SAR, China; Centre for Biosystems, Neuroscience, and Nanotechnology, City University of Hong Kong, Hong Kong SAR, China; Corresponding authorSummary: Using a well-established chronic visceral hypersensitivity (VH) rat model, we characterized the decrease of myelin basic protein, reduced number of mature oligodendrocytes (OLs), and hypomyelination in the anterior cingulate cortex (ACC). The results of rat gambling test showed impaired decision-making, and the results of electrophysiological studies showed desynchronization in the ACC to basolateral amygdala (BLA) neural circuitry. Astrocytes release various factors that modulate oligodendrocyte progenitor cell proliferation and myelination. Astrocytic Gq-modulation through expression of hM3Dq facilitated oligodendrocyte progenitor cell proliferation and OL differentiation, and enhanced ACC myelination in VH rats. Activating astrocytic Gq rescued impaired decision-making and desynchronization in ACC-BLA. These data indicate that ACC hypomyelination is an important component of impaired decision-making and network desynchronization in VH. Astrocytic Gq activity plays a significant role in oligodendrocyte myelination and decision-making behavior in VH. Insights from these studies have potential for interventions in myelin-related diseases such as chronic pain-associated cognitive disorders.http://www.sciencedirect.com/science/article/pii/S2589004222021137Biological sciencesCognitive neuroscienceNeuroscience |
| spellingShingle | Mahadi Hasan Zhuogui Lei Mastura Akter Zafar Iqbal Faeeqa Usaila Aruna Surendran Ramkrishnan Ying Li Chemogenetic activation of astrocytes promotes remyelination and restores cognitive deficits in visceral hypersensitive rats iScience Biological sciences Cognitive neuroscience Neuroscience |
| title | Chemogenetic activation of astrocytes promotes remyelination and restores cognitive deficits in visceral hypersensitive rats |
| title_full | Chemogenetic activation of astrocytes promotes remyelination and restores cognitive deficits in visceral hypersensitive rats |
| title_fullStr | Chemogenetic activation of astrocytes promotes remyelination and restores cognitive deficits in visceral hypersensitive rats |
| title_full_unstemmed | Chemogenetic activation of astrocytes promotes remyelination and restores cognitive deficits in visceral hypersensitive rats |
| title_short | Chemogenetic activation of astrocytes promotes remyelination and restores cognitive deficits in visceral hypersensitive rats |
| title_sort | chemogenetic activation of astrocytes promotes remyelination and restores cognitive deficits in visceral hypersensitive rats |
| topic | Biological sciences Cognitive neuroscience Neuroscience |
| url | http://www.sciencedirect.com/science/article/pii/S2589004222021137 |
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