Effects of Silver Nanoparticles on the Ovarian Tissue of NMRI Rats Treated with Alpha Lipoic Acid: A Stereological Study
Background and Objective: Silver nanoparticles are produced in large quantities in the industry and have estrogenic activities and toxic effects on different organs. This study was conducted to determine the effect of silver nanoparticles on the ovarian tissue of NMRI rats treated with alpha lipoic...
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| Format: | Article |
| Language: | fas |
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Golestan University of Medical Sciences
2023-12-01
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| Series: | مجله دانشگاه علوم پزشکی گرگان |
| Subjects: | |
| Online Access: | http://goums.ac.ir/journal/article-1-4195-en.pdf |
| _version_ | 1827380125128196096 |
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| author | Seyed Mohammad Ali Shariatzadeh Sara Aghabarati Parisa Maleki |
| author_facet | Seyed Mohammad Ali Shariatzadeh Sara Aghabarati Parisa Maleki |
| author_sort | Seyed Mohammad Ali Shariatzadeh |
| collection | DOAJ |
| description | Background and Objective: Silver nanoparticles are produced in large quantities in the industry and have estrogenic activities and toxic effects on different organs. This study was conducted to determine the effect of silver nanoparticles on the ovarian tissue of NMRI rats treated with alpha lipoic acid.
Methods: In this experimental study, 24 female NMRI rats were randomly divided into 4 groups of 6. The groups included the control group, oral silver nanoparticles (500 mg/kg of body weight), injected alpha lipoic acid (100 mg/kg of body weight), and silver nanoparticles (500 mg/kg of body weight) plus alpha lipoic acid (100 mg/kg body weight). The treatment was performed for 28 days. After the treatment period, blood sampling was performed from the rats’ hearts to analyze biochemical parameters (malondialdehyde, estrogen, progesterone, and total antioxidant capacity using the ferric reducing ability of plasma (FRAP) method). By dissecting the rats, the left ovaries were removed, fixed, molded, and cut, tissue passaging was performed, and the ovaries were stained using the hematoxylin-eosin method. Then, the ovarian tissue was evaluated by different stereological methods.
Results: The total mean ovarian volume, the cortex volume, the medulla volume, and the corpus luteum volume, and the total number of primordial, primary, secondary, and Graafian follicles were significantly reduced in the silver nanoparticles group compared to the control group (P<0.05). The simultaneous administration of alpha lipoic acid and silver nanoparticles compensated for the adverse effects of silver nanoparticles on the above parameters. On the other hand, the mean number of different types of follicles in the rats treated with alpha lipoic acid significantly increased compared to the control group (P<0.05). A statistically significant reduction was observed in the measurement of estrogen and progesterone hormones in the serum of the silver nanoparticles group compared to the control group (P<0.05). Moreover, in assessing the antioxidant capacity of the serum of the group treated simultaneously with silver nanoparticles + alpha lipoic acid, a statistically significant increase was observed compared to the group treated with silver nanoparticles (P<0.05).
Conclusion: Silver nanoparticles can have adverse effects on the structure of the ovary and its components, and alpha lipoic acid can largely compensate for these detrimental effects. |
| first_indexed | 2024-03-08T13:28:05Z |
| format | Article |
| id | doaj.art-0727a35e6e5d47209abc0b5a3d0b273f |
| institution | Directory Open Access Journal |
| issn | 1562-4765 2008-4080 |
| language | fas |
| last_indexed | 2024-03-08T13:28:05Z |
| publishDate | 2023-12-01 |
| publisher | Golestan University of Medical Sciences |
| record_format | Article |
| series | مجله دانشگاه علوم پزشکی گرگان |
| spelling | doaj.art-0727a35e6e5d47209abc0b5a3d0b273f2024-01-17T11:49:05ZfasGolestan University of Medical Sciencesمجله دانشگاه علوم پزشکی گرگان1562-47652008-40802023-12-012541118Effects of Silver Nanoparticles on the Ovarian Tissue of NMRI Rats Treated with Alpha Lipoic Acid: A Stereological StudySeyed Mohammad Ali Shariatzadeh0Sara Aghabarati1Parisa Maleki2 Professor, Department of Biology, Faculty of Science, Arak University, Arak, Iran. M.Sc in Biology, Department of Biology, Faculty of Science, Arak University, Arak, Iran. M.Sc in Biology, Academic Instructor, Department of Biology, Faculty of Science, Arak University, Arak, Iran. Background and Objective: Silver nanoparticles are produced in large quantities in the industry and have estrogenic activities and toxic effects on different organs. This study was conducted to determine the effect of silver nanoparticles on the ovarian tissue of NMRI rats treated with alpha lipoic acid. Methods: In this experimental study, 24 female NMRI rats were randomly divided into 4 groups of 6. The groups included the control group, oral silver nanoparticles (500 mg/kg of body weight), injected alpha lipoic acid (100 mg/kg of body weight), and silver nanoparticles (500 mg/kg of body weight) plus alpha lipoic acid (100 mg/kg body weight). The treatment was performed for 28 days. After the treatment period, blood sampling was performed from the rats’ hearts to analyze biochemical parameters (malondialdehyde, estrogen, progesterone, and total antioxidant capacity using the ferric reducing ability of plasma (FRAP) method). By dissecting the rats, the left ovaries were removed, fixed, molded, and cut, tissue passaging was performed, and the ovaries were stained using the hematoxylin-eosin method. Then, the ovarian tissue was evaluated by different stereological methods. Results: The total mean ovarian volume, the cortex volume, the medulla volume, and the corpus luteum volume, and the total number of primordial, primary, secondary, and Graafian follicles were significantly reduced in the silver nanoparticles group compared to the control group (P<0.05). The simultaneous administration of alpha lipoic acid and silver nanoparticles compensated for the adverse effects of silver nanoparticles on the above parameters. On the other hand, the mean number of different types of follicles in the rats treated with alpha lipoic acid significantly increased compared to the control group (P<0.05). A statistically significant reduction was observed in the measurement of estrogen and progesterone hormones in the serum of the silver nanoparticles group compared to the control group (P<0.05). Moreover, in assessing the antioxidant capacity of the serum of the group treated simultaneously with silver nanoparticles + alpha lipoic acid, a statistically significant increase was observed compared to the group treated with silver nanoparticles (P<0.05). Conclusion: Silver nanoparticles can have adverse effects on the structure of the ovary and its components, and alpha lipoic acid can largely compensate for these detrimental effects.http://goums.ac.ir/journal/article-1-4195-en.pdfnanoparticlessilverovaryalpha lipoic acidmice |
| spellingShingle | Seyed Mohammad Ali Shariatzadeh Sara Aghabarati Parisa Maleki Effects of Silver Nanoparticles on the Ovarian Tissue of NMRI Rats Treated with Alpha Lipoic Acid: A Stereological Study مجله دانشگاه علوم پزشکی گرگان nanoparticles silver ovary alpha lipoic acid mice |
| title | Effects of Silver Nanoparticles on the Ovarian Tissue of NMRI Rats Treated with Alpha Lipoic Acid: A Stereological Study |
| title_full | Effects of Silver Nanoparticles on the Ovarian Tissue of NMRI Rats Treated with Alpha Lipoic Acid: A Stereological Study |
| title_fullStr | Effects of Silver Nanoparticles on the Ovarian Tissue of NMRI Rats Treated with Alpha Lipoic Acid: A Stereological Study |
| title_full_unstemmed | Effects of Silver Nanoparticles on the Ovarian Tissue of NMRI Rats Treated with Alpha Lipoic Acid: A Stereological Study |
| title_short | Effects of Silver Nanoparticles on the Ovarian Tissue of NMRI Rats Treated with Alpha Lipoic Acid: A Stereological Study |
| title_sort | effects of silver nanoparticles on the ovarian tissue of nmri rats treated with alpha lipoic acid a stereological study |
| topic | nanoparticles silver ovary alpha lipoic acid mice |
| url | http://goums.ac.ir/journal/article-1-4195-en.pdf |
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