The Comparative Effects of Azanza garckeana Fruit Pulp and Melatonin on Serum and Testicular Oxidative Stress Changes, Live Sperm Cells and Spermatozoa Abnormalities Evoked by Chronic Administration of Bisphenol A in Rabbit Bucks
The study aimed to elucidate serum and testicular oxidative stress changes induced by bisphenol A (BPA) and their amelioration by Azanza garckeana (AG) pulp extract and melatonin. Adult New Zealand White rabbit bucks (n = 42), with average live weight of 1.2 ± 0.03 kg and aged 10-18 months...
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| Format: | Article |
| Language: | English |
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Faculty of Veterinary Medicine, Chiang Mai University
2023-02-01
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| Series: | Veterinary Integrative Sciences |
| Online Access: | https://he02.tci-thaijo.org/index.php/vis/article/view/261190 |
| _version_ | 1797782661304942592 |
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| author | Joy Iyojo Itodo Tagang Aluwong Chidiebere Uchendu Ngozi Ejum Ogbuagu John Shiradiyi Bugau Bode Abdulmujeeb Adewuyi Felix Uchenna Samuel Kenneth Owoicho Abah Matthew Shinkut Kanayo Peter Ogbuagu |
| author_facet | Joy Iyojo Itodo Tagang Aluwong Chidiebere Uchendu Ngozi Ejum Ogbuagu John Shiradiyi Bugau Bode Abdulmujeeb Adewuyi Felix Uchenna Samuel Kenneth Owoicho Abah Matthew Shinkut Kanayo Peter Ogbuagu |
| author_sort | Joy Iyojo Itodo |
| collection | DOAJ |
| description | The study aimed to elucidate serum and testicular oxidative stress changes induced by bisphenol A (BPA) and their amelioration by Azanza garckeana (AG) pulp extract and melatonin. Adult New Zealand White rabbit bucks (n = 42), with average live weight of 1.2 ± 0.03 kg and aged 10-18 months were fed ad libitum on a commercial diet. They were randomly divided into seven groups of six (6) bucks each. Group A was administered distilled water (1.5 mL); group B, BPA (100 mg/kg); group C, AG (500 mg/kg); group D, melatonin (1.0 mg/kg); group E was pre-dosed for six weeks with BPA (100 mg/kg), then AG (500 mg/kg) for another six weeks; group F was pre-dosed for six weeks with BPA (100 mg/kg ), then melatonin (1.0 mg/kg) for another six weeks; and group G was pre-dosed for six weeks with BPA (100 mg/kg), then AG (500 mg/kg) and melatonin(1.0 mg/kg) for another six weeks. There was a significant increase (p < 0.05) in the activities of both serum and testicular superoxide dismutase and catalase, concentrations of and reduced glutathione concentration as well as a decrease in MDA concentration in treatment groups. Percentage dead sperm, spermatozoa abnormalities such as detached sperm heads, free, coiled and bent tails in the groups exposed to BPA increased significantly (p < 0.05) compared to controls. It is concluded that BPA-induced oxidative stress. The administration of AG only ameliorated this negative effects better than melatonin. However, optimum results was seen when both substances were administered synergistically. |
| first_indexed | 2024-03-13T00:14:07Z |
| format | Article |
| id | doaj.art-072aca408b1b44d1b1c99c16881be228 |
| institution | Directory Open Access Journal |
| issn | 2629-9968 |
| language | English |
| last_indexed | 2024-03-13T00:14:07Z |
| publishDate | 2023-02-01 |
| publisher | Faculty of Veterinary Medicine, Chiang Mai University |
| record_format | Article |
| series | Veterinary Integrative Sciences |
| spelling | doaj.art-072aca408b1b44d1b1c99c16881be2282023-07-12T03:06:54ZengFaculty of Veterinary Medicine, Chiang Mai UniversityVeterinary Integrative Sciences2629-99682023-02-0121252354310.12982/VIS.2023.037The Comparative Effects of Azanza garckeana Fruit Pulp and Melatonin on Serum and Testicular Oxidative Stress Changes, Live Sperm Cells and Spermatozoa Abnormalities Evoked by Chronic Administration of Bisphenol A in Rabbit BucksJoy Iyojo ItodoTagang AluwongChidiebere UchenduNgozi Ejum OgbuaguJohn Shiradiyi BugauBode Abdulmujeeb AdewuyiFelix Uchenna SamuelKenneth Owoicho AbahMatthew ShinkutKanayo Peter Ogbuagu The study aimed to elucidate serum and testicular oxidative stress changes induced by bisphenol A (BPA) and their amelioration by Azanza garckeana (AG) pulp extract and melatonin. Adult New Zealand White rabbit bucks (n = 42), with average live weight of 1.2 ± 0.03 kg and aged 10-18 months were fed ad libitum on a commercial diet. They were randomly divided into seven groups of six (6) bucks each. Group A was administered distilled water (1.5 mL); group B, BPA (100 mg/kg); group C, AG (500 mg/kg); group D, melatonin (1.0 mg/kg); group E was pre-dosed for six weeks with BPA (100 mg/kg), then AG (500 mg/kg) for another six weeks; group F was pre-dosed for six weeks with BPA (100 mg/kg ), then melatonin (1.0 mg/kg) for another six weeks; and group G was pre-dosed for six weeks with BPA (100 mg/kg), then AG (500 mg/kg) and melatonin(1.0 mg/kg) for another six weeks. There was a significant increase (p < 0.05) in the activities of both serum and testicular superoxide dismutase and catalase, concentrations of and reduced glutathione concentration as well as a decrease in MDA concentration in treatment groups. Percentage dead sperm, spermatozoa abnormalities such as detached sperm heads, free, coiled and bent tails in the groups exposed to BPA increased significantly (p < 0.05) compared to controls. It is concluded that BPA-induced oxidative stress. The administration of AG only ameliorated this negative effects better than melatonin. However, optimum results was seen when both substances were administered synergistically.https://he02.tci-thaijo.org/index.php/vis/article/view/261190 |
| spellingShingle | Joy Iyojo Itodo Tagang Aluwong Chidiebere Uchendu Ngozi Ejum Ogbuagu John Shiradiyi Bugau Bode Abdulmujeeb Adewuyi Felix Uchenna Samuel Kenneth Owoicho Abah Matthew Shinkut Kanayo Peter Ogbuagu The Comparative Effects of Azanza garckeana Fruit Pulp and Melatonin on Serum and Testicular Oxidative Stress Changes, Live Sperm Cells and Spermatozoa Abnormalities Evoked by Chronic Administration of Bisphenol A in Rabbit Bucks Veterinary Integrative Sciences |
| title | The Comparative Effects of Azanza garckeana Fruit Pulp and Melatonin on Serum and Testicular Oxidative Stress Changes, Live Sperm Cells and Spermatozoa Abnormalities Evoked by Chronic Administration of Bisphenol A in Rabbit Bucks |
| title_full | The Comparative Effects of Azanza garckeana Fruit Pulp and Melatonin on Serum and Testicular Oxidative Stress Changes, Live Sperm Cells and Spermatozoa Abnormalities Evoked by Chronic Administration of Bisphenol A in Rabbit Bucks |
| title_fullStr | The Comparative Effects of Azanza garckeana Fruit Pulp and Melatonin on Serum and Testicular Oxidative Stress Changes, Live Sperm Cells and Spermatozoa Abnormalities Evoked by Chronic Administration of Bisphenol A in Rabbit Bucks |
| title_full_unstemmed | The Comparative Effects of Azanza garckeana Fruit Pulp and Melatonin on Serum and Testicular Oxidative Stress Changes, Live Sperm Cells and Spermatozoa Abnormalities Evoked by Chronic Administration of Bisphenol A in Rabbit Bucks |
| title_short | The Comparative Effects of Azanza garckeana Fruit Pulp and Melatonin on Serum and Testicular Oxidative Stress Changes, Live Sperm Cells and Spermatozoa Abnormalities Evoked by Chronic Administration of Bisphenol A in Rabbit Bucks |
| title_sort | comparative effects of azanza garckeana fruit pulp and melatonin on serum and testicular oxidative stress changes live sperm cells and spermatozoa abnormalities evoked by chronic administration of bisphenol a in rabbit bucks |
| url | https://he02.tci-thaijo.org/index.php/vis/article/view/261190 |
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