The Highly Repeat-Diverse (Peri) Centromeres of White Lupin (Lupinus albus L.)
Plant genomes are known to be mainly composed of repetitive DNA sequences. Regardless of the non-genic function of these sequences, they are important for chromosome structure and stability during cell-cycle. Based on the recent available whole-genome assembly of white lupin (Lupinus albus L.; WL),...
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| Format: | Article |
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Frontiers Media S.A.
2022-04-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.862079/full |
| _version_ | 1811311457717977088 |
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| author | André Marques Bárbara Hufnagel Alexandre Soriano Benjamin Péret |
| author_facet | André Marques Bárbara Hufnagel Alexandre Soriano Benjamin Péret |
| author_sort | André Marques |
| collection | DOAJ |
| description | Plant genomes are known to be mainly composed of repetitive DNA sequences. Regardless of the non-genic function of these sequences, they are important for chromosome structure and stability during cell-cycle. Based on the recent available whole-genome assembly of white lupin (Lupinus albus L.; WL), we have in silico annotated and in situ mapped the main classes of DNA repeats identified with RepeatExplorer. A highly diverse and an abundance of satellite DNAs were found representing more than 10 families, where three of them were highly associated with CENH3-immunoprecipitated chromatin. Applying a strategy of several re-hybridization steps with different combinations of satDNA, rDNA, and LTR-RTs probes, we were able to construct a repeat-based chromosome map for the identification of most chromosome pairs. Two families of LTR retrotransposons, Ty1/copia SIRE and Ty3/gypsy Tekay, were highly abundant at pericentromeric regions, while the centromeric retrotransposon of WL (CRWL) from the CRM clade showed strong centromere-specific localization in most chromosomes and was also highly enriched with CENH3-immunoprecipitated chromatin. FISH mapping of repeat DNA showed some incongruences with the reference genome, which can be further used for improving the current version of the genome. Our results demonstrate that despite the relatively small genome of WL, a high diversity of pericentromeric repeats was found, emphasizing the rapid evolution of repeat sequences in plant genomes. |
| first_indexed | 2024-04-13T10:19:14Z |
| format | Article |
| id | doaj.art-072d4f6576894537b46ffada11de5fbb |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-13T10:19:14Z |
| publishDate | 2022-04-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Plant Science |
| spelling | doaj.art-072d4f6576894537b46ffada11de5fbb2022-12-22T02:50:33ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-04-011310.3389/fpls.2022.862079862079The Highly Repeat-Diverse (Peri) Centromeres of White Lupin (Lupinus albus L.)André MarquesBárbara HufnagelAlexandre SorianoBenjamin PéretPlant genomes are known to be mainly composed of repetitive DNA sequences. Regardless of the non-genic function of these sequences, they are important for chromosome structure and stability during cell-cycle. Based on the recent available whole-genome assembly of white lupin (Lupinus albus L.; WL), we have in silico annotated and in situ mapped the main classes of DNA repeats identified with RepeatExplorer. A highly diverse and an abundance of satellite DNAs were found representing more than 10 families, where three of them were highly associated with CENH3-immunoprecipitated chromatin. Applying a strategy of several re-hybridization steps with different combinations of satDNA, rDNA, and LTR-RTs probes, we were able to construct a repeat-based chromosome map for the identification of most chromosome pairs. Two families of LTR retrotransposons, Ty1/copia SIRE and Ty3/gypsy Tekay, were highly abundant at pericentromeric regions, while the centromeric retrotransposon of WL (CRWL) from the CRM clade showed strong centromere-specific localization in most chromosomes and was also highly enriched with CENH3-immunoprecipitated chromatin. FISH mapping of repeat DNA showed some incongruences with the reference genome, which can be further used for improving the current version of the genome. Our results demonstrate that despite the relatively small genome of WL, a high diversity of pericentromeric repeats was found, emphasizing the rapid evolution of repeat sequences in plant genomes.https://www.frontiersin.org/articles/10.3389/fpls.2022.862079/fullheterochromatinrepetitive DNAcentromereCENH3satellite DNAcytogenomics |
| spellingShingle | André Marques Bárbara Hufnagel Alexandre Soriano Benjamin Péret The Highly Repeat-Diverse (Peri) Centromeres of White Lupin (Lupinus albus L.) Frontiers in Plant Science heterochromatin repetitive DNA centromere CENH3 satellite DNA cytogenomics |
| title | The Highly Repeat-Diverse (Peri) Centromeres of White Lupin (Lupinus albus L.) |
| title_full | The Highly Repeat-Diverse (Peri) Centromeres of White Lupin (Lupinus albus L.) |
| title_fullStr | The Highly Repeat-Diverse (Peri) Centromeres of White Lupin (Lupinus albus L.) |
| title_full_unstemmed | The Highly Repeat-Diverse (Peri) Centromeres of White Lupin (Lupinus albus L.) |
| title_short | The Highly Repeat-Diverse (Peri) Centromeres of White Lupin (Lupinus albus L.) |
| title_sort | highly repeat diverse peri centromeres of white lupin lupinus albus l |
| topic | heterochromatin repetitive DNA centromere CENH3 satellite DNA cytogenomics |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.862079/full |
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