Comparison of Fluidic and Non-Fluidic Surface Plasmon Resonance Biosensor Variants for Angular and Intensity Modulation Measurements
Fluidic and non-fluidic surface plasmon resonance measurements were realized for the same type of sensory layer and using the same mouse IgG antibody and anti-mouse IgG antibody biomolecular system. A comparison of the thicknesses of the anti-mouse IgG antibody layers bound to the ligand at increasi...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-12-01
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| Series: | Sensors |
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| Online Access: | https://www.mdpi.com/1424-8220/23/24/9899 |
| _version_ | 1797379333318246400 |
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| author | Piotr Mrozek Lukasz Oldak Ewa Gorodkiewicz |
| author_facet | Piotr Mrozek Lukasz Oldak Ewa Gorodkiewicz |
| author_sort | Piotr Mrozek |
| collection | DOAJ |
| description | Fluidic and non-fluidic surface plasmon resonance measurements were realized for the same type of sensory layer and using the same mouse IgG antibody and anti-mouse IgG antibody biomolecular system. A comparison of the thicknesses of the anti-mouse IgG antibody layers bound to the ligand at increasing analyte concentrations ranging from 0.0 μg mL<sup>−1</sup> to 5.0 μg mL<sup>−1</sup> in the non-fluidic and the fluidic variant showed that the thickness of the bound anti-mouse antibody layers in the fluidic variant was approximately 1.5–3 times larger than in the non-fluidic variant. The greater thicknesses of the deposited layers were also reflected in the larger increment of the resonant angle in the fluidic variant compared to the non-fluidic variant in the considered range of analyte concentrations. The choice between fluidic and non-fluidic surface plasmon resonance biosensors may be justified by the availability of analyte volume and the intended modulation technique. When working with limited analyte, non-fluidic biosensors with intensity modulation are more advantageous. For larger analyte quantities, fluidic biosensors with angular modulation are recommended, primarily due to their slightly higher sensitivity in this measurement mode. |
| first_indexed | 2024-03-08T20:21:38Z |
| format | Article |
| id | doaj.art-0731cd2ec48d46e4b541b0a16bad4f4c |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-03-08T20:21:38Z |
| publishDate | 2023-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-0731cd2ec48d46e4b541b0a16bad4f4c2023-12-22T14:41:37ZengMDPI AGSensors1424-82202023-12-012324989910.3390/s23249899Comparison of Fluidic and Non-Fluidic Surface Plasmon Resonance Biosensor Variants for Angular and Intensity Modulation MeasurementsPiotr Mrozek0Lukasz Oldak1Ewa Gorodkiewicz2Faculty of Mechanical Engineering, Bialystok University of Technology, Wiejska 45C, 15-351 Bialystok, PolandBioanalysis Laboratory, Faculty of Chemistry, University of Bialystok, Ciolkowskiego 1K, 15-245 Bialystok, PolandBioanalysis Laboratory, Faculty of Chemistry, University of Bialystok, Ciolkowskiego 1K, 15-245 Bialystok, PolandFluidic and non-fluidic surface plasmon resonance measurements were realized for the same type of sensory layer and using the same mouse IgG antibody and anti-mouse IgG antibody biomolecular system. A comparison of the thicknesses of the anti-mouse IgG antibody layers bound to the ligand at increasing analyte concentrations ranging from 0.0 μg mL<sup>−1</sup> to 5.0 μg mL<sup>−1</sup> in the non-fluidic and the fluidic variant showed that the thickness of the bound anti-mouse antibody layers in the fluidic variant was approximately 1.5–3 times larger than in the non-fluidic variant. The greater thicknesses of the deposited layers were also reflected in the larger increment of the resonant angle in the fluidic variant compared to the non-fluidic variant in the considered range of analyte concentrations. The choice between fluidic and non-fluidic surface plasmon resonance biosensors may be justified by the availability of analyte volume and the intended modulation technique. When working with limited analyte, non-fluidic biosensors with intensity modulation are more advantageous. For larger analyte quantities, fluidic biosensors with angular modulation are recommended, primarily due to their slightly higher sensitivity in this measurement mode.https://www.mdpi.com/1424-8220/23/24/9899Surface Plasmon Resonance (SPR)SPR biosensornon-fluidic and fluidic biosensorangular modulationintensity modulation |
| spellingShingle | Piotr Mrozek Lukasz Oldak Ewa Gorodkiewicz Comparison of Fluidic and Non-Fluidic Surface Plasmon Resonance Biosensor Variants for Angular and Intensity Modulation Measurements Sensors Surface Plasmon Resonance (SPR) SPR biosensor non-fluidic and fluidic biosensor angular modulation intensity modulation |
| title | Comparison of Fluidic and Non-Fluidic Surface Plasmon Resonance Biosensor Variants for Angular and Intensity Modulation Measurements |
| title_full | Comparison of Fluidic and Non-Fluidic Surface Plasmon Resonance Biosensor Variants for Angular and Intensity Modulation Measurements |
| title_fullStr | Comparison of Fluidic and Non-Fluidic Surface Plasmon Resonance Biosensor Variants for Angular and Intensity Modulation Measurements |
| title_full_unstemmed | Comparison of Fluidic and Non-Fluidic Surface Plasmon Resonance Biosensor Variants for Angular and Intensity Modulation Measurements |
| title_short | Comparison of Fluidic and Non-Fluidic Surface Plasmon Resonance Biosensor Variants for Angular and Intensity Modulation Measurements |
| title_sort | comparison of fluidic and non fluidic surface plasmon resonance biosensor variants for angular and intensity modulation measurements |
| topic | Surface Plasmon Resonance (SPR) SPR biosensor non-fluidic and fluidic biosensor angular modulation intensity modulation |
| url | https://www.mdpi.com/1424-8220/23/24/9899 |
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