Lactate Dehydrogenase and Oxidative Stress Activity in Primary Open-Angle Glaucoma Aqueous Humour
Lactate dehydrogenase (LDH) and lactate are some of the hypoxy biochemical parameters. Extracellular activity of this enzyme increases under the condition of oxidative stress, since the cell integrity can be disrupted during the lipid peroxidation process. Subsequently that leads to the increase lev...
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| Format: | Article |
| Language: | English |
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Association of Basic Medical Sciences of Federation of Bosnia and Herzegovina
2010-02-01
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| Series: | Biomolecules & Biomedicine |
| Subjects: | |
| Online Access: | https://www.bjbms.org/ojs/index.php/bjbms/article/view/2743 |
| _version_ | 1797260862081204224 |
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| author | Predrag Jovanović Lepša Žorić Ivan Stefanović Boban Džunić Jasmina Djordjević-Jocić Marija Radenković Maja Jovanovic |
| author_facet | Predrag Jovanović Lepša Žorić Ivan Stefanović Boban Džunić Jasmina Djordjević-Jocić Marija Radenković Maja Jovanovic |
| author_sort | Predrag Jovanović |
| collection | DOAJ |
| description | Lactate dehydrogenase (LDH) and lactate are some of the hypoxy biochemical parameters. Extracellular activity of this enzyme increases under the condition of oxidative stress, since the cell integrity can be disrupted during the lipid peroxidation process. Subsequently that leads to the increase level of the lactic acid and lactic acid salts. The objective of this investigation is establishing the level of LDH, LDH1 (HBDH) and the lactate concentration in aqueous humour in patients with primary open-angle glaucoma.
Biochemical analysis have been made by enzymatic-colometric method (lactate) and UV-kinetic method (LDH and HBDH) in aqueous humour of 30 patients (42 eyes) with primary open-angle glaucoma (POAG) and 30 patients (40 eyes) with cataract (the control group).
The increased values of lactate and the activity of LDH and HBDH enzyme in aqueous humour of POAG patients in correlation with the control group are the results not only of oxidative stress but also of hypoxy and the mitochondry oxidative function (p<0,001).
The increased activity of the examined biochemical parameters in the aqueous humour of the POAG patients points to the fact that other mechanisms, besides IOP, have a role in glaucoma pathogenesis.
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| first_indexed | 2024-04-24T23:32:04Z |
| format | Article |
| id | doaj.art-07350705e8344479aef7f265bb6fc8b7 |
| institution | Directory Open Access Journal |
| issn | 2831-0896 2831-090X |
| language | English |
| last_indexed | 2024-04-24T23:32:04Z |
| publishDate | 2010-02-01 |
| publisher | Association of Basic Medical Sciences of Federation of Bosnia and Herzegovina |
| record_format | Article |
| series | Biomolecules & Biomedicine |
| spelling | doaj.art-07350705e8344479aef7f265bb6fc8b72024-03-15T14:34:29ZengAssociation of Basic Medical Sciences of Federation of Bosnia and HerzegovinaBiomolecules & Biomedicine2831-08962831-090X2010-02-0110110.17305/bjbms.2010.2743454Lactate Dehydrogenase and Oxidative Stress Activity in Primary Open-Angle Glaucoma Aqueous HumourPredrag Jovanović0Lepša Žorić1Ivan Stefanović2Boban Džunić3Jasmina Djordjević-Jocić4Marija Radenković5Maja Jovanovic6Ophthalmology Clinic, Clinical CenterDepartment of Ophthalmology, Faculty of MedicineInstitute for Eye Diseases, Clinical Center of SerbiaOphthalmology Clinic, Clinical CenterOphthalmology Clinic, Clinical CenterOphthalmology Clinic, Clinical CenterClinic for Infective DiseasesLactate dehydrogenase (LDH) and lactate are some of the hypoxy biochemical parameters. Extracellular activity of this enzyme increases under the condition of oxidative stress, since the cell integrity can be disrupted during the lipid peroxidation process. Subsequently that leads to the increase level of the lactic acid and lactic acid salts. The objective of this investigation is establishing the level of LDH, LDH1 (HBDH) and the lactate concentration in aqueous humour in patients with primary open-angle glaucoma. Biochemical analysis have been made by enzymatic-colometric method (lactate) and UV-kinetic method (LDH and HBDH) in aqueous humour of 30 patients (42 eyes) with primary open-angle glaucoma (POAG) and 30 patients (40 eyes) with cataract (the control group). The increased values of lactate and the activity of LDH and HBDH enzyme in aqueous humour of POAG patients in correlation with the control group are the results not only of oxidative stress but also of hypoxy and the mitochondry oxidative function (p<0,001). The increased activity of the examined biochemical parameters in the aqueous humour of the POAG patients points to the fact that other mechanisms, besides IOP, have a role in glaucoma pathogenesis. https://www.bjbms.org/ojs/index.php/bjbms/article/view/2743glaucomaoxidative stressischaemialactate dehydrogenaselactates |
| spellingShingle | Predrag Jovanović Lepša Žorić Ivan Stefanović Boban Džunić Jasmina Djordjević-Jocić Marija Radenković Maja Jovanovic Lactate Dehydrogenase and Oxidative Stress Activity in Primary Open-Angle Glaucoma Aqueous Humour Biomolecules & Biomedicine glaucoma oxidative stress ischaemia lactate dehydrogenase lactates |
| title | Lactate Dehydrogenase and Oxidative Stress Activity in Primary Open-Angle Glaucoma Aqueous Humour |
| title_full | Lactate Dehydrogenase and Oxidative Stress Activity in Primary Open-Angle Glaucoma Aqueous Humour |
| title_fullStr | Lactate Dehydrogenase and Oxidative Stress Activity in Primary Open-Angle Glaucoma Aqueous Humour |
| title_full_unstemmed | Lactate Dehydrogenase and Oxidative Stress Activity in Primary Open-Angle Glaucoma Aqueous Humour |
| title_short | Lactate Dehydrogenase and Oxidative Stress Activity in Primary Open-Angle Glaucoma Aqueous Humour |
| title_sort | lactate dehydrogenase and oxidative stress activity in primary open angle glaucoma aqueous humour |
| topic | glaucoma oxidative stress ischaemia lactate dehydrogenase lactates |
| url | https://www.bjbms.org/ojs/index.php/bjbms/article/view/2743 |
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