Induction of Apoptosis in Cancer Cells of pre-B ALL Patients after Exposure to Platelets, Platelet-Derived Microparticles and Soluble CD40 Ligand
Objective The in vitro treatment of tumor cells with platelet (Plt) causes inhibition of tumor cell growth, although mechanism of this effect is not clear yet. Induction of apoptosis has been proposed as a mechanism of Plt effects on tumor cells. The purpose of this study was to clarify the role of...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Royan Institute (ACECR), Tehran
2018-01-01
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| Series: | Cell Journal |
| Subjects: | |
| Online Access: | http://celljournal.org/journal/article/17795/download |
| _version_ | 1811204643793928192 |
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| author | Morteza Yaftian Fatemeh Yari Mehran Ghasemzadeh Vahid Fallah Azad Mansoureh Haghighi |
| author_facet | Morteza Yaftian Fatemeh Yari Mehran Ghasemzadeh Vahid Fallah Azad Mansoureh Haghighi |
| author_sort | Morteza Yaftian |
| collection | DOAJ |
| description | Objective
The in vitro treatment of tumor cells with platelet (Plt) causes inhibition of tumor cell growth, although mechanism of this effect is not clear yet. Induction of apoptosis has been proposed as a mechanism of Plt effects on tumor cells. The purpose of this study was to clarify the role of Plts and Plt-derived components in the induction of apoptosis in the blood mononuclear cells of patients with leukemia.
Materials and Methods
In this experimental study, peripheral blood mononuclear cells (PBMCs) were isolated from whole blood of five patients with childhood B-precursor acute lymphoblastic leukemia (pre-B ALL) and encountered with Plts, Plt-derived microparticles (Plt-MPs) as well as purified soluble CD40L (sCD40L). After 48 hours of co-culture, the anti-cancer activity of the aforementioned factors was surveyed using examination of apoptosis markers of the cells including active caspase-3 and CD95 using ELISA and flow cytometer techniques, respectively. Additionally, staining of the cells with 7-Aminoactinomycin D (7-AAD) was evaluated by flow cytometer technique. Trypan blue exclusion test and WST-1 method were also used to compare the death/survival status of the cells.
Results
Levels of CD95 and caspase-3 were significantly increased in the all treated groups (P<0.05). On the other hand, trypan blue, 7-AAD and WST-1 methods showed significantly lower number of the live cells in the treated groups (P<0.05).
Conclusion
This study can show the ability of Plts, Plt-MPs and sCD40L for the induction of apoptosis in PBMCs of pre-B-ALL patients. Further studies are necessary to elucidate the different effects of platelets on cancer cells in vitro and in vivo. |
| first_indexed | 2024-04-12T03:17:05Z |
| format | Article |
| id | doaj.art-07356d73fd20478daf867fa89760159a |
| institution | Directory Open Access Journal |
| issn | 2228-5806 2228-5814 |
| language | English |
| last_indexed | 2024-04-12T03:17:05Z |
| publishDate | 2018-01-01 |
| publisher | Royan Institute (ACECR), Tehran |
| record_format | Article |
| series | Cell Journal |
| spelling | doaj.art-07356d73fd20478daf867fa89760159a2022-12-22T03:50:03ZengRoyan Institute (ACECR), TehranCell Journal2228-58062228-58142018-01-0120112012610.22074/cellj.2018.5032Induction of Apoptosis in Cancer Cells of pre-B ALL Patients after Exposure to Platelets, Platelet-Derived Microparticles and Soluble CD40 LigandMorteza Yaftian0Fatemeh Yari1Mehran Ghasemzadeh2Vahid Fallah Azad3Mansoureh Haghighi4Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, IranBlood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, IranBlood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, IranMahak Pediatric Cancer Research and Hospital Center, Tehran, IranMahak Pediatric Cancer Research and Hospital Center, Tehran, IranObjective The in vitro treatment of tumor cells with platelet (Plt) causes inhibition of tumor cell growth, although mechanism of this effect is not clear yet. Induction of apoptosis has been proposed as a mechanism of Plt effects on tumor cells. The purpose of this study was to clarify the role of Plts and Plt-derived components in the induction of apoptosis in the blood mononuclear cells of patients with leukemia. Materials and Methods In this experimental study, peripheral blood mononuclear cells (PBMCs) were isolated from whole blood of five patients with childhood B-precursor acute lymphoblastic leukemia (pre-B ALL) and encountered with Plts, Plt-derived microparticles (Plt-MPs) as well as purified soluble CD40L (sCD40L). After 48 hours of co-culture, the anti-cancer activity of the aforementioned factors was surveyed using examination of apoptosis markers of the cells including active caspase-3 and CD95 using ELISA and flow cytometer techniques, respectively. Additionally, staining of the cells with 7-Aminoactinomycin D (7-AAD) was evaluated by flow cytometer technique. Trypan blue exclusion test and WST-1 method were also used to compare the death/survival status of the cells. Results Levels of CD95 and caspase-3 were significantly increased in the all treated groups (P<0.05). On the other hand, trypan blue, 7-AAD and WST-1 methods showed significantly lower number of the live cells in the treated groups (P<0.05). Conclusion This study can show the ability of Plts, Plt-MPs and sCD40L for the induction of apoptosis in PBMCs of pre-B-ALL patients. Further studies are necessary to elucidate the different effects of platelets on cancer cells in vitro and in vivo.http://celljournal.org/journal/article/17795/downloadAcute Lymphoblastic LeukemiaApoptosisCD40 LigandMicroparticlesPlatelet |
| spellingShingle | Morteza Yaftian Fatemeh Yari Mehran Ghasemzadeh Vahid Fallah Azad Mansoureh Haghighi Induction of Apoptosis in Cancer Cells of pre-B ALL Patients after Exposure to Platelets, Platelet-Derived Microparticles and Soluble CD40 Ligand Cell Journal Acute Lymphoblastic Leukemia Apoptosis CD40 Ligand Microparticles Platelet |
| title | Induction of Apoptosis in Cancer Cells of pre-B ALL Patients after Exposure to Platelets, Platelet-Derived Microparticles and Soluble CD40 Ligand |
| title_full | Induction of Apoptosis in Cancer Cells of pre-B ALL Patients after Exposure to Platelets, Platelet-Derived Microparticles and Soluble CD40 Ligand |
| title_fullStr | Induction of Apoptosis in Cancer Cells of pre-B ALL Patients after Exposure to Platelets, Platelet-Derived Microparticles and Soluble CD40 Ligand |
| title_full_unstemmed | Induction of Apoptosis in Cancer Cells of pre-B ALL Patients after Exposure to Platelets, Platelet-Derived Microparticles and Soluble CD40 Ligand |
| title_short | Induction of Apoptosis in Cancer Cells of pre-B ALL Patients after Exposure to Platelets, Platelet-Derived Microparticles and Soluble CD40 Ligand |
| title_sort | induction of apoptosis in cancer cells of pre b all patients after exposure to platelets platelet derived microparticles and soluble cd40 ligand |
| topic | Acute Lymphoblastic Leukemia Apoptosis CD40 Ligand Microparticles Platelet |
| url | http://celljournal.org/journal/article/17795/download |
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