Anti-Inflammatory Effects of <i>Limosilactobacillus fermentum</i> KGC1601 Isolated from <i>Panax ginseng</i> and Its Probiotic Characteristics
We investigated the potential probiotic properties of <i>Limosilactobacillus fermentum</i> KGC1601 isolated from <i>Panax ginseng</i>. Ginseng cultivated in an experimental field of the Korea Ginseng Research Institute was fermented, followed by single colony selection from M...
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MDPI AG
2022-06-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/11/12/1707 |
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| author | Heejin Kim Yun-Seok Lee Hye-Young Yu Mijin Kwon Ki-Kwang Kim Gyo In Soon-Ki Hong Sang-Kyu Kim |
| author_facet | Heejin Kim Yun-Seok Lee Hye-Young Yu Mijin Kwon Ki-Kwang Kim Gyo In Soon-Ki Hong Sang-Kyu Kim |
| author_sort | Heejin Kim |
| collection | DOAJ |
| description | We investigated the potential probiotic properties of <i>Limosilactobacillus fermentum</i> KGC1601 isolated from <i>Panax ginseng</i>. Ginseng cultivated in an experimental field of the Korea Ginseng Research Institute was fermented, followed by single colony selection from MRS agar. We performed 16s-rRNA sequencing and whole-genome analysis to identify <i>L. fermentum</i> and evaluate the biosafety parameters of this strain, respectively. We confirmed this strain was susceptible to six antibiotics, as proposed by the European Food Safety Authority, did not produce biogenic amines, and did not exhibit any hemolytic activity. Acid resistance and bile salt tolerance, which are essential properties of a probiotic agent, were investigated. Notably, distinguishing properties of this strain were that it exhibited excellent bile salt tolerance and anti-inflammatory effects. The excellent bile salt tolerance was confirmed by scanning electron microscopy. Through qRT-PCR and ELISA studies, it was revealed that <i>L. fermentum</i> KGC1601 pre-treatment up-regulates anti-inflammatory cytokines and down-regulates pro-inflammatory cytokines in RAW 264.7 cells. Consequently, we suggested that <i>L. fermentum</i> KGC1601 can be safely used as a potential anti-inflammatory functional probiotic agent. |
| first_indexed | 2024-03-09T23:49:18Z |
| format | Article |
| id | doaj.art-073986b98dc74f499034e540650b4fb4 |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-09T23:49:18Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-073986b98dc74f499034e540650b4fb42023-11-23T16:37:46ZengMDPI AGFoods2304-81582022-06-011112170710.3390/foods11121707Anti-Inflammatory Effects of <i>Limosilactobacillus fermentum</i> KGC1601 Isolated from <i>Panax ginseng</i> and Its Probiotic CharacteristicsHeejin Kim0Yun-Seok Lee1Hye-Young Yu2Mijin Kwon3Ki-Kwang Kim4Gyo In5Soon-Ki Hong6Sang-Kyu Kim7Laboratory of Red Ginseng Products, Korea Ginseng Corporation, 30, Gajeong-ro, Shinseong-dong, Yuseong-gu, Daejeon 34128, KoreaLaboratory of Red Ginseng Products, Korea Ginseng Corporation, 30, Gajeong-ro, Shinseong-dong, Yuseong-gu, Daejeon 34128, KoreaLaboratory of Red Ginseng Products, Korea Ginseng Corporation, 30, Gajeong-ro, Shinseong-dong, Yuseong-gu, Daejeon 34128, KoreaLaboratory of Red Ginseng Products, Korea Ginseng Corporation, 30, Gajeong-ro, Shinseong-dong, Yuseong-gu, Daejeon 34128, KoreaDepartment of Environmental Engineering, Chungnam National University, Daehak-ro, Yuseong-gu, Daejeon 34134, KoreaLaboratory of Red Ginseng Products, Korea Ginseng Corporation, 30, Gajeong-ro, Shinseong-dong, Yuseong-gu, Daejeon 34128, KoreaLaboratory of Efficacy Research, Korea Ginseng Corporation, 30, Gajeong-ro, Shinseong-dong, Yuseong-gu, Daejeon 34128, KoreaLaboratory of Red Ginseng Products, Korea Ginseng Corporation, 30, Gajeong-ro, Shinseong-dong, Yuseong-gu, Daejeon 34128, KoreaWe investigated the potential probiotic properties of <i>Limosilactobacillus fermentum</i> KGC1601 isolated from <i>Panax ginseng</i>. Ginseng cultivated in an experimental field of the Korea Ginseng Research Institute was fermented, followed by single colony selection from MRS agar. We performed 16s-rRNA sequencing and whole-genome analysis to identify <i>L. fermentum</i> and evaluate the biosafety parameters of this strain, respectively. We confirmed this strain was susceptible to six antibiotics, as proposed by the European Food Safety Authority, did not produce biogenic amines, and did not exhibit any hemolytic activity. Acid resistance and bile salt tolerance, which are essential properties of a probiotic agent, were investigated. Notably, distinguishing properties of this strain were that it exhibited excellent bile salt tolerance and anti-inflammatory effects. The excellent bile salt tolerance was confirmed by scanning electron microscopy. Through qRT-PCR and ELISA studies, it was revealed that <i>L. fermentum</i> KGC1601 pre-treatment up-regulates anti-inflammatory cytokines and down-regulates pro-inflammatory cytokines in RAW 264.7 cells. Consequently, we suggested that <i>L. fermentum</i> KGC1601 can be safely used as a potential anti-inflammatory functional probiotic agent.https://www.mdpi.com/2304-8158/11/12/1707anti-inflammatorylactic acid bacteria<i>Limosilactobacillus fermentum</i><i>Panax ginseng</i>probiotics |
| spellingShingle | Heejin Kim Yun-Seok Lee Hye-Young Yu Mijin Kwon Ki-Kwang Kim Gyo In Soon-Ki Hong Sang-Kyu Kim Anti-Inflammatory Effects of <i>Limosilactobacillus fermentum</i> KGC1601 Isolated from <i>Panax ginseng</i> and Its Probiotic Characteristics Foods anti-inflammatory lactic acid bacteria <i>Limosilactobacillus fermentum</i> <i>Panax ginseng</i> probiotics |
| title | Anti-Inflammatory Effects of <i>Limosilactobacillus fermentum</i> KGC1601 Isolated from <i>Panax ginseng</i> and Its Probiotic Characteristics |
| title_full | Anti-Inflammatory Effects of <i>Limosilactobacillus fermentum</i> KGC1601 Isolated from <i>Panax ginseng</i> and Its Probiotic Characteristics |
| title_fullStr | Anti-Inflammatory Effects of <i>Limosilactobacillus fermentum</i> KGC1601 Isolated from <i>Panax ginseng</i> and Its Probiotic Characteristics |
| title_full_unstemmed | Anti-Inflammatory Effects of <i>Limosilactobacillus fermentum</i> KGC1601 Isolated from <i>Panax ginseng</i> and Its Probiotic Characteristics |
| title_short | Anti-Inflammatory Effects of <i>Limosilactobacillus fermentum</i> KGC1601 Isolated from <i>Panax ginseng</i> and Its Probiotic Characteristics |
| title_sort | anti inflammatory effects of i limosilactobacillus fermentum i kgc1601 isolated from i panax ginseng i and its probiotic characteristics |
| topic | anti-inflammatory lactic acid bacteria <i>Limosilactobacillus fermentum</i> <i>Panax ginseng</i> probiotics |
| url | https://www.mdpi.com/2304-8158/11/12/1707 |
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