Genomic Characterization of <i>Escherichia coli</i> Isolates Belonging to a New Hybrid aEPEC/ExPEC Pathotype O153:H10-A-ST10 <i>eae</i>-beta1 Occurred in Meat, Poultry, Wildlife and Human Diarrheagenic Samples
Different surveillance studies (2005–2015) in northwest Spain revealed the presence of <i>eae</i>-positive isolates of <i>Escherichia coli</i> O153:H10 in meat for human consumption, poultry farm, wildlife and human diarrheagenic samples. The aim of this study was to explore...
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MDPI AG
2020-04-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/9/4/192 |
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| author | Dafne Díaz-Jiménez Isidro García-Meniño Alexandra Herrera Vanesa García Ana María López-Beceiro María Pilar Alonso Jorge Blanco Azucena Mora |
| author_facet | Dafne Díaz-Jiménez Isidro García-Meniño Alexandra Herrera Vanesa García Ana María López-Beceiro María Pilar Alonso Jorge Blanco Azucena Mora |
| author_sort | Dafne Díaz-Jiménez |
| collection | DOAJ |
| description | Different surveillance studies (2005–2015) in northwest Spain revealed the presence of <i>eae</i>-positive isolates of <i>Escherichia coli</i> O153:H10 in meat for human consumption, poultry farm, wildlife and human diarrheagenic samples. The aim of this study was to explore the genetic and genomic relatedness between human and animal/meat isolates, as well as the mechanism of its persistence. We also wanted to know whether it was a geographically restricted lineage, or whether it was also reported elsewhere. Conventional typing showed that 32 isolates were O153:H10-A-ST10 <i>fim</i>H54, <i>fimAv<sub>MT78</sub></i>, <i>traT</i> and <i>eae</i>-beta1. Amongst these, 21 were CTX-M-32 or SHV-12 producers. The PFGE <i>XbaI</i>-macrorestriction comparison showed high similarity (>85%). The plasmidome analysis revealed a stable combination of IncF (F2:A-:B-), IncI1 (STunknown) and IncX1 plasmid types, together with non-conjugative Col-like plasmids. The core genome investigation based on the cgMLST scheme from EnteroBase proved close relatedness between isolates of human and animal origin. Our results demonstrate that a hybrid MDR aEPEC/ExPEC of the clonal group O153:H10-A-ST10 (CH11-54) is circulating in our region within different hosts, including wildlife. It seems implicated in human diarrhea via meat transmission, and in the spreading of ESBL genes (mainly of CTX-M-32 type). We found genomic evidence of a related hybrid aEPEC/ExPEC in at least one other country. |
| first_indexed | 2024-03-10T20:23:48Z |
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| id | doaj.art-073e9c2870d04711b4fcdc71a313096b |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-10T20:23:48Z |
| publishDate | 2020-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-073e9c2870d04711b4fcdc71a313096b2023-11-19T21:58:04ZengMDPI AGAntibiotics2079-63822020-04-019419210.3390/antibiotics9040192Genomic Characterization of <i>Escherichia coli</i> Isolates Belonging to a New Hybrid aEPEC/ExPEC Pathotype O153:H10-A-ST10 <i>eae</i>-beta1 Occurred in Meat, Poultry, Wildlife and Human Diarrheagenic SamplesDafne Díaz-Jiménez0Isidro García-Meniño1Alexandra Herrera2Vanesa García3Ana María López-Beceiro4María Pilar Alonso5Jorge Blanco6Azucena Mora7Laboratorio de Referencia de Escherichia coli (LREC), Departamento de Microbioloxía e Parasitoloxía, Facultade de Veterinaria, Universidade de Santiago de Compostela (USC), 27002 Lugo, SpainLaboratorio de Referencia de Escherichia coli (LREC), Departamento de Microbioloxía e Parasitoloxía, Facultade de Veterinaria, Universidade de Santiago de Compostela (USC), 27002 Lugo, SpainLaboratorio de Referencia de Escherichia coli (LREC), Departamento de Microbioloxía e Parasitoloxía, Facultade de Veterinaria, Universidade de Santiago de Compostela (USC), 27002 Lugo, SpainLaboratorio de Referencia de Escherichia coli (LREC), Departamento de Microbioloxía e Parasitoloxía, Facultade de Veterinaria, Universidade de Santiago de Compostela (USC), 27002 Lugo, SpainDepartamento de Anatomía, Produción Animal e Ciencias Clínicas Veterinarias, Facultade de Veterinaria, Universidade de Santiago de Compostela (USC), 27002 Lugo, SpainUnidade de Microbioloxía, Hospital Universitario Lucus Augusti (HULA), 27003 Lugo, SpainLaboratorio de Referencia de Escherichia coli (LREC), Departamento de Microbioloxía e Parasitoloxía, Facultade de Veterinaria, Universidade de Santiago de Compostela (USC), 27002 Lugo, SpainLaboratorio de Referencia de Escherichia coli (LREC), Departamento de Microbioloxía e Parasitoloxía, Facultade de Veterinaria, Universidade de Santiago de Compostela (USC), 27002 Lugo, SpainDifferent surveillance studies (2005–2015) in northwest Spain revealed the presence of <i>eae</i>-positive isolates of <i>Escherichia coli</i> O153:H10 in meat for human consumption, poultry farm, wildlife and human diarrheagenic samples. The aim of this study was to explore the genetic and genomic relatedness between human and animal/meat isolates, as well as the mechanism of its persistence. We also wanted to know whether it was a geographically restricted lineage, or whether it was also reported elsewhere. Conventional typing showed that 32 isolates were O153:H10-A-ST10 <i>fim</i>H54, <i>fimAv<sub>MT78</sub></i>, <i>traT</i> and <i>eae</i>-beta1. Amongst these, 21 were CTX-M-32 or SHV-12 producers. The PFGE <i>XbaI</i>-macrorestriction comparison showed high similarity (>85%). The plasmidome analysis revealed a stable combination of IncF (F2:A-:B-), IncI1 (STunknown) and IncX1 plasmid types, together with non-conjugative Col-like plasmids. The core genome investigation based on the cgMLST scheme from EnteroBase proved close relatedness between isolates of human and animal origin. Our results demonstrate that a hybrid MDR aEPEC/ExPEC of the clonal group O153:H10-A-ST10 (CH11-54) is circulating in our region within different hosts, including wildlife. It seems implicated in human diarrhea via meat transmission, and in the spreading of ESBL genes (mainly of CTX-M-32 type). We found genomic evidence of a related hybrid aEPEC/ExPEC in at least one other country.https://www.mdpi.com/2079-6382/9/4/192<i>Escherichia coli</i>ESBLhybrid pathotypeExPECEPECMDR |
| spellingShingle | Dafne Díaz-Jiménez Isidro García-Meniño Alexandra Herrera Vanesa García Ana María López-Beceiro María Pilar Alonso Jorge Blanco Azucena Mora Genomic Characterization of <i>Escherichia coli</i> Isolates Belonging to a New Hybrid aEPEC/ExPEC Pathotype O153:H10-A-ST10 <i>eae</i>-beta1 Occurred in Meat, Poultry, Wildlife and Human Diarrheagenic Samples Antibiotics <i>Escherichia coli</i> ESBL hybrid pathotype ExPEC EPEC MDR |
| title | Genomic Characterization of <i>Escherichia coli</i> Isolates Belonging to a New Hybrid aEPEC/ExPEC Pathotype O153:H10-A-ST10 <i>eae</i>-beta1 Occurred in Meat, Poultry, Wildlife and Human Diarrheagenic Samples |
| title_full | Genomic Characterization of <i>Escherichia coli</i> Isolates Belonging to a New Hybrid aEPEC/ExPEC Pathotype O153:H10-A-ST10 <i>eae</i>-beta1 Occurred in Meat, Poultry, Wildlife and Human Diarrheagenic Samples |
| title_fullStr | Genomic Characterization of <i>Escherichia coli</i> Isolates Belonging to a New Hybrid aEPEC/ExPEC Pathotype O153:H10-A-ST10 <i>eae</i>-beta1 Occurred in Meat, Poultry, Wildlife and Human Diarrheagenic Samples |
| title_full_unstemmed | Genomic Characterization of <i>Escherichia coli</i> Isolates Belonging to a New Hybrid aEPEC/ExPEC Pathotype O153:H10-A-ST10 <i>eae</i>-beta1 Occurred in Meat, Poultry, Wildlife and Human Diarrheagenic Samples |
| title_short | Genomic Characterization of <i>Escherichia coli</i> Isolates Belonging to a New Hybrid aEPEC/ExPEC Pathotype O153:H10-A-ST10 <i>eae</i>-beta1 Occurred in Meat, Poultry, Wildlife and Human Diarrheagenic Samples |
| title_sort | genomic characterization of i escherichia coli i isolates belonging to a new hybrid aepec expec pathotype o153 h10 a st10 i eae i beta1 occurred in meat poultry wildlife and human diarrheagenic samples |
| topic | <i>Escherichia coli</i> ESBL hybrid pathotype ExPEC EPEC MDR |
| url | https://www.mdpi.com/2079-6382/9/4/192 |
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