<i>Treponema</i> spp. Isolated from Bovine Digital Dermatitis Display Different Pathogenicity in a Murine Abscess Model
Digital dermatitis (DD) causes lameness in cattle with substantial negative impact on sustainability and animal welfare. Although several species of <i>Treponema</i> bacteria have been isolated from various DD stages, their individual or synergistic roles in the initiation or development...
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MDPI AG
2020-09-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/8/10/1507 |
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| author | Rakel Arrazuria Cameron G. Knight Priyoshi Lahiri Eduardo R. Cobo Herman W. Barkema Jeroen De Buck |
| author_facet | Rakel Arrazuria Cameron G. Knight Priyoshi Lahiri Eduardo R. Cobo Herman W. Barkema Jeroen De Buck |
| author_sort | Rakel Arrazuria |
| collection | DOAJ |
| description | Digital dermatitis (DD) causes lameness in cattle with substantial negative impact on sustainability and animal welfare. Although several species of <i>Treponema</i> bacteria have been isolated from various DD stages, their individual or synergistic roles in the initiation or development of lesions remain largely unknown. The objective of this study was to compare effects of the three most common <i>Treponema</i> species isolated from DD lesions in cattle (<i>T. phagedenis, T. medium and T. pedis</i>), both as individual and as mixed inoculations, in a murine abscess model. A total of 10<sup>9</sup> or 5 × 10<sup>8</sup><i>Treponema</i> spp. were inoculated subcutaneously, and produced abscess was studied after 7 days post infection. There were no synergistic effects when two or three species were inoculated together; however, <i>T. medium</i> produced the largest abscesses, whereas those produced by <i>T. phagedenis</i> were the smallest and least severe. <i>Treponema</i> species were cultured from skin lesions at 7 days post infection and, additionally, from the kidneys of some mice (2/5), confirming systemic infection may occur. Taken together, these findings suggest that <i>T. medium</i> and <i>T. pedis</i> may have more important roles in DD lesion initiation and development than <i>T. phagedenis</i>. |
| first_indexed | 2024-03-10T15:56:30Z |
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| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T15:56:30Z |
| publishDate | 2020-09-01 |
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| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-073ef5e659c74d079b6afd0b5b1318a12023-11-20T15:35:56ZengMDPI AGMicroorganisms2076-26072020-09-01810150710.3390/microorganisms8101507<i>Treponema</i> spp. Isolated from Bovine Digital Dermatitis Display Different Pathogenicity in a Murine Abscess ModelRakel Arrazuria0Cameron G. Knight1Priyoshi Lahiri2Eduardo R. Cobo3Herman W. Barkema4Jeroen De Buck5Department of Production Animal Health, Faculty of Veterinary Medicine, University of Calgary, Calgary, AB T2N1N4, CanadaDepartment of Veterinary Clinical and Diagnostic Sciences, Veterinary Medicine, University of Calgary, Calgary, AB T2N1N4, CanadaDepartment of Production Animal Health, Faculty of Veterinary Medicine, University of Calgary, Calgary, AB T2N1N4, CanadaDepartment of Production Animal Health, Faculty of Veterinary Medicine, University of Calgary, Calgary, AB T2N1N4, CanadaDepartment of Production Animal Health, Faculty of Veterinary Medicine, University of Calgary, Calgary, AB T2N1N4, CanadaDepartment of Production Animal Health, Faculty of Veterinary Medicine, University of Calgary, Calgary, AB T2N1N4, CanadaDigital dermatitis (DD) causes lameness in cattle with substantial negative impact on sustainability and animal welfare. Although several species of <i>Treponema</i> bacteria have been isolated from various DD stages, their individual or synergistic roles in the initiation or development of lesions remain largely unknown. The objective of this study was to compare effects of the three most common <i>Treponema</i> species isolated from DD lesions in cattle (<i>T. phagedenis, T. medium and T. pedis</i>), both as individual and as mixed inoculations, in a murine abscess model. A total of 10<sup>9</sup> or 5 × 10<sup>8</sup><i>Treponema</i> spp. were inoculated subcutaneously, and produced abscess was studied after 7 days post infection. There were no synergistic effects when two or three species were inoculated together; however, <i>T. medium</i> produced the largest abscesses, whereas those produced by <i>T. phagedenis</i> were the smallest and least severe. <i>Treponema</i> species were cultured from skin lesions at 7 days post infection and, additionally, from the kidneys of some mice (2/5), confirming systemic infection may occur. Taken together, these findings suggest that <i>T. medium</i> and <i>T. pedis</i> may have more important roles in DD lesion initiation and development than <i>T. phagedenis</i>.https://www.mdpi.com/2076-2607/8/10/1507<i>Treponema phagedenis</i><i>Treponema pedis</i><i>Treponema medium</i>digital dermatitismouse abscess model |
| spellingShingle | Rakel Arrazuria Cameron G. Knight Priyoshi Lahiri Eduardo R. Cobo Herman W. Barkema Jeroen De Buck <i>Treponema</i> spp. Isolated from Bovine Digital Dermatitis Display Different Pathogenicity in a Murine Abscess Model Microorganisms <i>Treponema phagedenis</i> <i>Treponema pedis</i> <i>Treponema medium</i> digital dermatitis mouse abscess model |
| title | <i>Treponema</i> spp. Isolated from Bovine Digital Dermatitis Display Different Pathogenicity in a Murine Abscess Model |
| title_full | <i>Treponema</i> spp. Isolated from Bovine Digital Dermatitis Display Different Pathogenicity in a Murine Abscess Model |
| title_fullStr | <i>Treponema</i> spp. Isolated from Bovine Digital Dermatitis Display Different Pathogenicity in a Murine Abscess Model |
| title_full_unstemmed | <i>Treponema</i> spp. Isolated from Bovine Digital Dermatitis Display Different Pathogenicity in a Murine Abscess Model |
| title_short | <i>Treponema</i> spp. Isolated from Bovine Digital Dermatitis Display Different Pathogenicity in a Murine Abscess Model |
| title_sort | i treponema i spp isolated from bovine digital dermatitis display different pathogenicity in a murine abscess model |
| topic | <i>Treponema phagedenis</i> <i>Treponema pedis</i> <i>Treponema medium</i> digital dermatitis mouse abscess model |
| url | https://www.mdpi.com/2076-2607/8/10/1507 |
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