Detection of Dibutyl Phthalate in Surface Water by Fluorescence Polarization Immunoassay
Dibutyl phthalate (DBP) is widely used as a plasticizer in the production of polymeric materials to give them flexibility, strength and extensibility. However, due to its negative impact on human health, in particular reproductive functions and fetal development, the content of DBP must be controlle...
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MDPI AG
2023-11-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/13/12/1005 |
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| author | Liliya I. Mukhametova Madina R. Karimova Olga G. Zharikova Andrey V. Pirogov Valentina V. Levkina Ekaterina S. Chichkanova Liqiang Liu Chuanlai Xu Sergei A. Eremin |
| author_facet | Liliya I. Mukhametova Madina R. Karimova Olga G. Zharikova Andrey V. Pirogov Valentina V. Levkina Ekaterina S. Chichkanova Liqiang Liu Chuanlai Xu Sergei A. Eremin |
| author_sort | Liliya I. Mukhametova |
| collection | DOAJ |
| description | Dibutyl phthalate (DBP) is widely used as a plasticizer in the production of polymeric materials to give them flexibility, strength and extensibility. However, due to its negative impact on human health, in particular reproductive functions and fetal development, the content of DBP must be controlled in food and the environment. The present study aims to develop a sensitive, fast and simple fluorescence polarization immunoassay (FPIA) using monoclonal antibodies derived against DBP (MAb-DBP) for its detection in open waters. New conjugates of DBP with various fluorescein derivatives were obtained and characterized: 5-aminomethylfluorescein (AMF) and dichlorotriazinylaminofluorescein (DTAF). The advantages of using the DBP-AMF conjugate in the FPIA method are shown, the kinetics of binding of this chemical with antibodies are studied, the analysis is optimized, and the concentration of monoclonal antibodies is selected for sensitivity analysis—16 nM. The calibration dependence of the fluorescence polarization signal for the detection of DBP was obtained. The observed IC50 (DBP concentration at which a 50% decrease in the fluorescence polarization signal occurs, 40 ng/mL) and the limit of detection (LOD, 7.5 ng/mL) values were improved by a factor of 45 over the previously described FPIA using polyclonal antibodies. This technique was tested by the recovery method, and the high percentage of DBP discovery in water ranged from 85 to 110%. Using the developed method, real water samples from Lake Onega were tested, and a good correlation was shown between the results of the determination of DBP by the FPIA method and GC-MS. Thus, the FPIA method developed in this work can be used to determine DBP in open-water reservoirs. |
| first_indexed | 2024-03-08T20:57:21Z |
| format | Article |
| id | doaj.art-073f47a08e61449fa0896d9c48d879c6 |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-08T20:57:21Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-073f47a08e61449fa0896d9c48d879c62023-12-22T13:56:18ZengMDPI AGBiosensors2079-63742023-11-011312100510.3390/bios13121005Detection of Dibutyl Phthalate in Surface Water by Fluorescence Polarization ImmunoassayLiliya I. Mukhametova0Madina R. Karimova1Olga G. Zharikova2Andrey V. Pirogov3Valentina V. Levkina4Ekaterina S. Chichkanova5Liqiang Liu6Chuanlai Xu7Sergei A. Eremin8Faculty of Chemistry, M. V. Lomonosov Moscow State University, Leninskie Gory 1, 119991 Moscow, RussiaFaculty of Chemistry, M. V. Lomonosov Moscow State University, Leninskie Gory 1, 119991 Moscow, RussiaFaculty of Chemistry, M. V. Lomonosov Moscow State University, Leninskie Gory 1, 119991 Moscow, RussiaFaculty of Chemistry, M. V. Lomonosov Moscow State University, Leninskie Gory 1, 119991 Moscow, RussiaFaculty of Chemistry, M. V. Lomonosov Moscow State University, Leninskie Gory 1, 119991 Moscow, RussiaFaculty of Chemistry, M. V. Lomonosov Moscow State University, Leninskie Gory 1, 119991 Moscow, RussiaSchool of Food Science and Technology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, ChinaSchool of Food Science and Technology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, ChinaFaculty of Chemistry, M. V. Lomonosov Moscow State University, Leninskie Gory 1, 119991 Moscow, RussiaDibutyl phthalate (DBP) is widely used as a plasticizer in the production of polymeric materials to give them flexibility, strength and extensibility. However, due to its negative impact on human health, in particular reproductive functions and fetal development, the content of DBP must be controlled in food and the environment. The present study aims to develop a sensitive, fast and simple fluorescence polarization immunoassay (FPIA) using monoclonal antibodies derived against DBP (MAb-DBP) for its detection in open waters. New conjugates of DBP with various fluorescein derivatives were obtained and characterized: 5-aminomethylfluorescein (AMF) and dichlorotriazinylaminofluorescein (DTAF). The advantages of using the DBP-AMF conjugate in the FPIA method are shown, the kinetics of binding of this chemical with antibodies are studied, the analysis is optimized, and the concentration of monoclonal antibodies is selected for sensitivity analysis—16 nM. The calibration dependence of the fluorescence polarization signal for the detection of DBP was obtained. The observed IC50 (DBP concentration at which a 50% decrease in the fluorescence polarization signal occurs, 40 ng/mL) and the limit of detection (LOD, 7.5 ng/mL) values were improved by a factor of 45 over the previously described FPIA using polyclonal antibodies. This technique was tested by the recovery method, and the high percentage of DBP discovery in water ranged from 85 to 110%. Using the developed method, real water samples from Lake Onega were tested, and a good correlation was shown between the results of the determination of DBP by the FPIA method and GC-MS. Thus, the FPIA method developed in this work can be used to determine DBP in open-water reservoirs.https://www.mdpi.com/2079-6374/13/12/1005fluorescence polarization immunoassayphthalatesdibutyl phthalatecontaminationwater safety |
| spellingShingle | Liliya I. Mukhametova Madina R. Karimova Olga G. Zharikova Andrey V. Pirogov Valentina V. Levkina Ekaterina S. Chichkanova Liqiang Liu Chuanlai Xu Sergei A. Eremin Detection of Dibutyl Phthalate in Surface Water by Fluorescence Polarization Immunoassay Biosensors fluorescence polarization immunoassay phthalates dibutyl phthalate contamination water safety |
| title | Detection of Dibutyl Phthalate in Surface Water by Fluorescence Polarization Immunoassay |
| title_full | Detection of Dibutyl Phthalate in Surface Water by Fluorescence Polarization Immunoassay |
| title_fullStr | Detection of Dibutyl Phthalate in Surface Water by Fluorescence Polarization Immunoassay |
| title_full_unstemmed | Detection of Dibutyl Phthalate in Surface Water by Fluorescence Polarization Immunoassay |
| title_short | Detection of Dibutyl Phthalate in Surface Water by Fluorescence Polarization Immunoassay |
| title_sort | detection of dibutyl phthalate in surface water by fluorescence polarization immunoassay |
| topic | fluorescence polarization immunoassay phthalates dibutyl phthalate contamination water safety |
| url | https://www.mdpi.com/2079-6374/13/12/1005 |
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