Tracking B cell responses to the SARS-CoV-2 mRNA-1273 vaccine
Summary: Protective immunity following vaccination is sustained by long-lived antibody-secreting cells and resting memory B cells (MBCs). Responses to two-dose SARS-CoV-2 mRNA-1273 vaccination are evaluated longitudinally by multimodal single-cell analysis in three infection-naïve individuals. Integ...
| Main Authors: | , , , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-07-01
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| Series: | Cell Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S221112472300791X |
| _version_ | 1797780654083014656 |
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| author | Felipe Lopes de Assis Kenneth B. Hoehn Xiaozhen Zhang Lela Kardava Connor D. Smith Omar El Merhebi Clarisa M. Buckner Krittin Trihemasava Wei Wang Catherine A. Seamon Vicky Chen Paul Schaughency Foo Cheung Andrew J. Martins Chi-I Chiang Yuxing Li John S. Tsang Tae-Wook Chun Steven H. Kleinstein Susan Moir |
| author_facet | Felipe Lopes de Assis Kenneth B. Hoehn Xiaozhen Zhang Lela Kardava Connor D. Smith Omar El Merhebi Clarisa M. Buckner Krittin Trihemasava Wei Wang Catherine A. Seamon Vicky Chen Paul Schaughency Foo Cheung Andrew J. Martins Chi-I Chiang Yuxing Li John S. Tsang Tae-Wook Chun Steven H. Kleinstein Susan Moir |
| author_sort | Felipe Lopes de Assis |
| collection | DOAJ |
| description | Summary: Protective immunity following vaccination is sustained by long-lived antibody-secreting cells and resting memory B cells (MBCs). Responses to two-dose SARS-CoV-2 mRNA-1273 vaccination are evaluated longitudinally by multimodal single-cell analysis in three infection-naïve individuals. Integrated surface protein, transcriptomics, and B cell receptor (BCR) repertoire analysis of sorted plasmablasts and spike+ (S-2P+) and S-2P− B cells reveal clonal expansion and accumulating mutations among S-2P+ cells. These cells are enriched in a cluster of immunoglobulin G-expressing MBCs and evolve along a bifurcated trajectory rooted in CXCR3+ MBCs. One branch leads to CD11c+ atypical MBCs while the other develops from CD71+ activated precursors to resting MBCs, the dominant population at month 6. Among 12 evolving S-2P+ clones, several are populated with plasmablasts at early timepoints as well as CD71+ activated and resting MBCs at later timepoints, and display intra- and/or inter-cohort BCR convergence. These relationships suggest a coordinated and predictable evolution of SARS-CoV-2 vaccine-generated MBCs. |
| first_indexed | 2024-03-12T23:47:02Z |
| format | Article |
| id | doaj.art-0741d21fc63c4181b8b82bf41955424e |
| institution | Directory Open Access Journal |
| issn | 2211-1247 |
| language | English |
| last_indexed | 2024-03-12T23:47:02Z |
| publishDate | 2023-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports |
| spelling | doaj.art-0741d21fc63c4181b8b82bf41955424e2023-07-14T04:27:46ZengElsevierCell Reports2211-12472023-07-01427112780Tracking B cell responses to the SARS-CoV-2 mRNA-1273 vaccineFelipe Lopes de Assis0Kenneth B. Hoehn1Xiaozhen Zhang2Lela Kardava3Connor D. Smith4Omar El Merhebi5Clarisa M. Buckner6Krittin Trihemasava7Wei Wang8Catherine A. Seamon9Vicky Chen10Paul Schaughency11Foo Cheung12Andrew J. Martins13Chi-I Chiang14Yuxing Li15John S. Tsang16Tae-Wook Chun17Steven H. Kleinstein18Susan Moir19Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USADepartment of Pathology, Yale School of Medicine, New Haven, CT 06520, USALaboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USALaboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USALaboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USALaboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USALaboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USALaboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USALaboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USALaboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USAIntegrated Data Sciences Section, Research Technologies Branch, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USAIntegrated Data Sciences Section, Research Technologies Branch, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USANIH Center for Human Immunology, NIAID, NIH, Bethesda, MD 20892, USAMultiscale Systems Biology Section, Laboratory of Immune System Biology, NIAID, NIH, Bethesda, MD 20892, USAInstitute for Bioscience and Biotechnology Research, Rockville, MD 20850, USAInstitute for Bioscience and Biotechnology Research, Rockville, MD 20850, USA; Department of Microbiology and Immunology and Center for Biomolecular Therapeutics, University of Maryland School of Medicine, Baltimore, MD 21201, USANIH Center for Human Immunology, NIAID, NIH, Bethesda, MD 20892, USA; Multiscale Systems Biology Section, Laboratory of Immune System Biology, NIAID, NIH, Bethesda, MD 20892, USALaboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USADepartment of Pathology, Yale School of Medicine, New Haven, CT 06520, USA; Program in Computational Biology and Bioinformatics, Yale University, New Haven, CT 06511, USA; Department of Immunobiology, Yale School of Medicine, New Haven, CT 06520, USALaboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA; Corresponding authorSummary: Protective immunity following vaccination is sustained by long-lived antibody-secreting cells and resting memory B cells (MBCs). Responses to two-dose SARS-CoV-2 mRNA-1273 vaccination are evaluated longitudinally by multimodal single-cell analysis in three infection-naïve individuals. Integrated surface protein, transcriptomics, and B cell receptor (BCR) repertoire analysis of sorted plasmablasts and spike+ (S-2P+) and S-2P− B cells reveal clonal expansion and accumulating mutations among S-2P+ cells. These cells are enriched in a cluster of immunoglobulin G-expressing MBCs and evolve along a bifurcated trajectory rooted in CXCR3+ MBCs. One branch leads to CD11c+ atypical MBCs while the other develops from CD71+ activated precursors to resting MBCs, the dominant population at month 6. Among 12 evolving S-2P+ clones, several are populated with plasmablasts at early timepoints as well as CD71+ activated and resting MBCs at later timepoints, and display intra- and/or inter-cohort BCR convergence. These relationships suggest a coordinated and predictable evolution of SARS-CoV-2 vaccine-generated MBCs.http://www.sciencedirect.com/science/article/pii/S221112472300791XCP: Immunology |
| spellingShingle | Felipe Lopes de Assis Kenneth B. Hoehn Xiaozhen Zhang Lela Kardava Connor D. Smith Omar El Merhebi Clarisa M. Buckner Krittin Trihemasava Wei Wang Catherine A. Seamon Vicky Chen Paul Schaughency Foo Cheung Andrew J. Martins Chi-I Chiang Yuxing Li John S. Tsang Tae-Wook Chun Steven H. Kleinstein Susan Moir Tracking B cell responses to the SARS-CoV-2 mRNA-1273 vaccine Cell Reports CP: Immunology |
| title | Tracking B cell responses to the SARS-CoV-2 mRNA-1273 vaccine |
| title_full | Tracking B cell responses to the SARS-CoV-2 mRNA-1273 vaccine |
| title_fullStr | Tracking B cell responses to the SARS-CoV-2 mRNA-1273 vaccine |
| title_full_unstemmed | Tracking B cell responses to the SARS-CoV-2 mRNA-1273 vaccine |
| title_short | Tracking B cell responses to the SARS-CoV-2 mRNA-1273 vaccine |
| title_sort | tracking b cell responses to the sars cov 2 mrna 1273 vaccine |
| topic | CP: Immunology |
| url | http://www.sciencedirect.com/science/article/pii/S221112472300791X |
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