Rapid Identification for Serotyping of African Swine Fever Virus Based on the Short Fragment of the EP402R Gene Encoding for CD2-Like Protein
The first confirmed case of African swine fever (ASF) in Vietnam was officially reported in February 2019. To date, the ASF virus (ASFV) has been detected in all 63 cities/provinces in Vietnam. In order to get a better understanding of the potential role of the EP402R gene in a grouping of ASFV sero...
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| Format: | Article |
| Language: | English |
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Sciendo
2021-03-01
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| Series: | Acta Veterinaria |
| Subjects: | |
| Online Access: | https://doi.org/10.2478/acve-2021-0007 |
| _version_ | 1819002938314457088 |
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| author | Thanh Tran Ha Thi Duc Truong Anh Viet Ly Duc Van Hoang Tuan Thi Nguyen Chinh Thi Chu Nhu Thi Nguyen Huyen Vu Dang Hoang |
| author_facet | Thanh Tran Ha Thi Duc Truong Anh Viet Ly Duc Van Hoang Tuan Thi Nguyen Chinh Thi Chu Nhu Thi Nguyen Huyen Vu Dang Hoang |
| author_sort | Thanh Tran Ha Thi |
| collection | DOAJ |
| description | The first confirmed case of African swine fever (ASF) in Vietnam was officially reported in February 2019. To date, the ASF virus (ASFV) has been detected in all 63 cities/provinces in Vietnam. In order to get a better understanding of the potential role of the EP402R gene in a grouping of ASFV serotypes, thirty ASFV sequences of EP402R genes (accession numbers: MN711757-86) from North Central Coast of Vietnam and 68 well-known references of serotype groups from previous studies were further analyzed. Interestingly, we found that a short fragment of 90 nucleotides was very typical for 8 serological groups of ASFVs. A primer set was designed to amplicon the short fragment of 90 nucleotides using the Primer3 program to establish a simplified method for the serotyping of ASFV. Our results indicated that phylogenetic analysis of the short fragment (90 nucleotides) of the EP402R gene is a very specific and useful method for ASFV serotyping when compared to the previous method using a long fragment (816 nucleotides) of this gene and well-known serotype references based on haemadsorption inhibition (HAI) assay. |
| first_indexed | 2024-12-20T23:13:03Z |
| format | Article |
| id | doaj.art-0742b5c0f3824b1888eec3f2d0db5fd2 |
| institution | Directory Open Access Journal |
| issn | 1820-7448 |
| language | English |
| last_indexed | 2024-12-20T23:13:03Z |
| publishDate | 2021-03-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Acta Veterinaria |
| spelling | doaj.art-0742b5c0f3824b1888eec3f2d0db5fd22022-12-21T19:23:42ZengSciendoActa Veterinaria1820-74482021-03-017119810610.2478/acve-2021-0007Rapid Identification for Serotyping of African Swine Fever Virus Based on the Short Fragment of the EP402R Gene Encoding for CD2-Like ProteinThanh Tran Ha Thi0Duc Truong Anh1Viet Ly Duc2Van Hoang Tuan3Thi Nguyen Chinh4Thi Chu Nhu5Thi Nguyen Huyen6Vu Dang Hoang7Department of Biochemistry and Immunology, National Institute of Veterinary Research, 86 Truong Chinh, Dong Da, Hanoi 100000, VietnamDepartment of Biochemistry and Immunology, National Institute of Veterinary Research, 86 Truong Chinh, Dong Da, Hanoi 100000, VietnamDepartment of Biochemistry and Immunology, National Institute of Veterinary Research, 86 Truong Chinh, Dong Da, Hanoi 100000, VietnamDepartment of Biochemistry and Immunology, National Institute of Veterinary Research, 86 Truong Chinh, Dong Da, Hanoi 100000, VietnamDepartment of Biochemistry and Immunology, National Institute of Veterinary Research, 86 Truong Chinh, Dong Da, Hanoi 100000, VietnamDepartment of Biochemistry and Immunology, National Institute of Veterinary Research, 86 Truong Chinh, Dong Da, Hanoi 100000, VietnamDepartment of Biochemistry and Immunology, National Institute of Veterinary Research, 86 Truong Chinh, Dong Da, Hanoi 100000, VietnamDepartment of Biochemistry and Immunology, National Institute of Veterinary Research, 86 Truong Chinh, Dong Da, Hanoi 100000, VietnamThe first confirmed case of African swine fever (ASF) in Vietnam was officially reported in February 2019. To date, the ASF virus (ASFV) has been detected in all 63 cities/provinces in Vietnam. In order to get a better understanding of the potential role of the EP402R gene in a grouping of ASFV serotypes, thirty ASFV sequences of EP402R genes (accession numbers: MN711757-86) from North Central Coast of Vietnam and 68 well-known references of serotype groups from previous studies were further analyzed. Interestingly, we found that a short fragment of 90 nucleotides was very typical for 8 serological groups of ASFVs. A primer set was designed to amplicon the short fragment of 90 nucleotides using the Primer3 program to establish a simplified method for the serotyping of ASFV. Our results indicated that phylogenetic analysis of the short fragment (90 nucleotides) of the EP402R gene is a very specific and useful method for ASFV serotyping when compared to the previous method using a long fragment (816 nucleotides) of this gene and well-known serotype references based on haemadsorption inhibition (HAI) assay.https://doi.org/10.2478/acve-2021-0007african swine fever virusserotypingep402r genecd2vvietnam |
| spellingShingle | Thanh Tran Ha Thi Duc Truong Anh Viet Ly Duc Van Hoang Tuan Thi Nguyen Chinh Thi Chu Nhu Thi Nguyen Huyen Vu Dang Hoang Rapid Identification for Serotyping of African Swine Fever Virus Based on the Short Fragment of the EP402R Gene Encoding for CD2-Like Protein Acta Veterinaria african swine fever virus serotyping ep402r gene cd2v vietnam |
| title | Rapid Identification for Serotyping of African Swine Fever Virus Based on the Short Fragment of the EP402R Gene Encoding for CD2-Like Protein |
| title_full | Rapid Identification for Serotyping of African Swine Fever Virus Based on the Short Fragment of the EP402R Gene Encoding for CD2-Like Protein |
| title_fullStr | Rapid Identification for Serotyping of African Swine Fever Virus Based on the Short Fragment of the EP402R Gene Encoding for CD2-Like Protein |
| title_full_unstemmed | Rapid Identification for Serotyping of African Swine Fever Virus Based on the Short Fragment of the EP402R Gene Encoding for CD2-Like Protein |
| title_short | Rapid Identification for Serotyping of African Swine Fever Virus Based on the Short Fragment of the EP402R Gene Encoding for CD2-Like Protein |
| title_sort | rapid identification for serotyping of african swine fever virus based on the short fragment of the ep402r gene encoding for cd2 like protein |
| topic | african swine fever virus serotyping ep402r gene cd2v vietnam |
| url | https://doi.org/10.2478/acve-2021-0007 |
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