Supplementation of Freezing Medium with Ginseng Improves Rooster Sperm Quality and Fertility Relative to Free Radicals and Antioxidant Enzymes
To the best of our knowledge, this study is the first to determine the effect of ginseng as an antioxidant supplement in freezing extenders on the quality of cryopreserved rooster semen. Semen samples were collected from 40 Thai native roosters (Pradu Hang Dum) using the dorso-abdominal massage meth...
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| Format: | Article |
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MDPI AG
2023-08-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/13/16/2660 |
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| author | Ruthaiporn Ratchamak Supakorn Authaida Thirawat Koedkanmark Wuttigrai Boonkum Yoswaris Semaming Vibuntita Chankitisakul |
| author_facet | Ruthaiporn Ratchamak Supakorn Authaida Thirawat Koedkanmark Wuttigrai Boonkum Yoswaris Semaming Vibuntita Chankitisakul |
| author_sort | Ruthaiporn Ratchamak |
| collection | DOAJ |
| description | To the best of our knowledge, this study is the first to determine the effect of ginseng as an antioxidant supplement in freezing extenders on the quality of cryopreserved rooster semen. Semen samples were collected from 40 Thai native roosters (Pradu Hang Dum) using the dorso-abdominal massage method and then pooled and divided into five groups according to the concentrations of ginseng supplementation (0, 0.25, 0.50, 0.75, and 1 mg/mL) in a freezing extender. The semen suspensions were loaded into a medium straw and cryopreserved using the liquid nitrogen vapor method. The post-thaw semen was evaluated for sperm quality (sperm motility and membrane integrity), seminal plasma characteristics (lipid peroxidation, superoxide dismutase [SOD], catalase [CAT], and glutathione peroxidase [GPx]), and fertility. The results showed that ginseng extract supplementation at 0.25 mg/mL yielded the highest total motility, progressive motility, and membrane integrity (59.47%, 30.82%, and 48.30%, respectively; <i>p</i> < 0.05) in cryopreserved rooster semen. Higher malondialdehyde concentrations were observed in the control group than in the other groups (<i>p</i> < 0.05). SOD, CAT, and GPx increased compared with those in the control group (<i>p</i> < 0.05). The results showed that the fertility rate with 0.25 mg/mL of ginseng was higher than that of the control group (62.80% vs. 46.28%: <i>p</i> < 0.05). In conclusion, supplementation with 0.25 mg/mL of ginseng is recommended as an alternative component to the freezing extender to improve rooster semen cryopreservation. |
| first_indexed | 2024-03-11T00:11:08Z |
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| id | doaj.art-074729640c6b438ba6316d8b670da924 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-11T00:11:08Z |
| publishDate | 2023-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-074729640c6b438ba6316d8b670da9242023-11-18T23:58:21ZengMDPI AGAnimals2076-26152023-08-011316266010.3390/ani13162660Supplementation of Freezing Medium with Ginseng Improves Rooster Sperm Quality and Fertility Relative to Free Radicals and Antioxidant EnzymesRuthaiporn Ratchamak0Supakorn Authaida1Thirawat Koedkanmark2Wuttigrai Boonkum3Yoswaris Semaming4Vibuntita Chankitisakul5Department of Animal Science, Faculty of Agricultural, Khon Kaen University, Khon Kaen 40002, ThailandDepartment of Animal Science, Faculty of Agricultural, Khon Kaen University, Khon Kaen 40002, ThailandDepartment of Animal Science, Faculty of Agricultural, Khon Kaen University, Khon Kaen 40002, ThailandDepartment of Animal Science, Faculty of Agricultural, Khon Kaen University, Khon Kaen 40002, ThailandProgram in Veterinary Technology, Faculty of Technology, Udon Thani Rajabhat University, Udon Thani 41000, ThailandDepartment of Animal Science, Faculty of Agricultural, Khon Kaen University, Khon Kaen 40002, ThailandTo the best of our knowledge, this study is the first to determine the effect of ginseng as an antioxidant supplement in freezing extenders on the quality of cryopreserved rooster semen. Semen samples were collected from 40 Thai native roosters (Pradu Hang Dum) using the dorso-abdominal massage method and then pooled and divided into five groups according to the concentrations of ginseng supplementation (0, 0.25, 0.50, 0.75, and 1 mg/mL) in a freezing extender. The semen suspensions were loaded into a medium straw and cryopreserved using the liquid nitrogen vapor method. The post-thaw semen was evaluated for sperm quality (sperm motility and membrane integrity), seminal plasma characteristics (lipid peroxidation, superoxide dismutase [SOD], catalase [CAT], and glutathione peroxidase [GPx]), and fertility. The results showed that ginseng extract supplementation at 0.25 mg/mL yielded the highest total motility, progressive motility, and membrane integrity (59.47%, 30.82%, and 48.30%, respectively; <i>p</i> < 0.05) in cryopreserved rooster semen. Higher malondialdehyde concentrations were observed in the control group than in the other groups (<i>p</i> < 0.05). SOD, CAT, and GPx increased compared with those in the control group (<i>p</i> < 0.05). The results showed that the fertility rate with 0.25 mg/mL of ginseng was higher than that of the control group (62.80% vs. 46.28%: <i>p</i> < 0.05). In conclusion, supplementation with 0.25 mg/mL of ginseng is recommended as an alternative component to the freezing extender to improve rooster semen cryopreservation.https://www.mdpi.com/2076-2615/13/16/2660cryopreservationfertilitylipid peroxidationseminal plasma characteristicsThai native chicken |
| spellingShingle | Ruthaiporn Ratchamak Supakorn Authaida Thirawat Koedkanmark Wuttigrai Boonkum Yoswaris Semaming Vibuntita Chankitisakul Supplementation of Freezing Medium with Ginseng Improves Rooster Sperm Quality and Fertility Relative to Free Radicals and Antioxidant Enzymes Animals cryopreservation fertility lipid peroxidation seminal plasma characteristics Thai native chicken |
| title | Supplementation of Freezing Medium with Ginseng Improves Rooster Sperm Quality and Fertility Relative to Free Radicals and Antioxidant Enzymes |
| title_full | Supplementation of Freezing Medium with Ginseng Improves Rooster Sperm Quality and Fertility Relative to Free Radicals and Antioxidant Enzymes |
| title_fullStr | Supplementation of Freezing Medium with Ginseng Improves Rooster Sperm Quality and Fertility Relative to Free Radicals and Antioxidant Enzymes |
| title_full_unstemmed | Supplementation of Freezing Medium with Ginseng Improves Rooster Sperm Quality and Fertility Relative to Free Radicals and Antioxidant Enzymes |
| title_short | Supplementation of Freezing Medium with Ginseng Improves Rooster Sperm Quality and Fertility Relative to Free Radicals and Antioxidant Enzymes |
| title_sort | supplementation of freezing medium with ginseng improves rooster sperm quality and fertility relative to free radicals and antioxidant enzymes |
| topic | cryopreservation fertility lipid peroxidation seminal plasma characteristics Thai native chicken |
| url | https://www.mdpi.com/2076-2615/13/16/2660 |
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