Integrated analysis of long non-coding RNAs and mRNAs associated with glaucoma in vitro
IntroductionIn recent years, the biological functions and important roles of long non-coding RNAs (lncRNAs) have been widely reported in many diseases. Although glaucoma is the leading cause of blindness worldwide, the specific mechanisms of lncRNAs in the pathogenesis and progression of glaucoma re...
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| Format: | Article |
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Frontiers Media S.A.
2023-02-01
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| Series: | Frontiers in Endocrinology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fendo.2023.1087442/full |
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| author | Mengling You Mengling You Mengling You Rong Rong Rong Rong Rong Rong Zhou Zeng Zhou Zeng Zhou Zeng Cong Fan Cong Fan Cong Fan Haibo Li Haibo Li Haibo Li Qian Yang Qian Yang Qian Yang Dan Ji Dan Ji Dan Ji |
| author_facet | Mengling You Mengling You Mengling You Rong Rong Rong Rong Rong Rong Zhou Zeng Zhou Zeng Zhou Zeng Cong Fan Cong Fan Cong Fan Haibo Li Haibo Li Haibo Li Qian Yang Qian Yang Qian Yang Dan Ji Dan Ji Dan Ji |
| author_sort | Mengling You |
| collection | DOAJ |
| description | IntroductionIn recent years, the biological functions and important roles of long non-coding RNAs (lncRNAs) have been widely reported in many diseases. Although glaucoma is the leading cause of blindness worldwide, the specific mechanisms of lncRNAs in the pathogenesis and progression of glaucoma remain unclear. Our research aims to elucidate the differentially expressed lncRNAs and mRNAs in glaucoma and to provide a basis for further exploration of the specific mechanism of action of lncRNAs in the progression of glaucoma.MethodsWe performed RNA sequencing on samples from a pressurized model of R28 cells and performed bioinformatics analyses on the sequencing results. The expression consistency of lncRNAs in clinical samples from patients with glaucoma or cataracts was detected using real-time quantitative polymerase chain reaction (RT-qPCR).ResultsRNA sequencing results showed that lncRNAs in cluster 5 were upregulated with increasing stress after typing all significantly altered lncRNAs using k-means in a cellular stress model. KEGG analysis indicated that they were associated with neurodegenerative diseases. Differentially expressed lncRNAs were verified by RT-qPCR, and the lncRNA expression levels of AC120246.2 and XLOC_006247 were significantly higher in the aqueous humor (AH) of patients with glaucoma than in those with cataracts. For LOC102551819, there was almost no expression in the AH and trabecular meshwork in patients with glaucoma but high expression was observed in the iris. ConclusionOur research proposes potential diagnostic or intervention targets for clinical applications as well as a theoretical basis for more in-depth research on the function of lncRNAs in glaucoma. |
| first_indexed | 2024-04-10T07:06:50Z |
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| id | doaj.art-074f5fa952174212822f95a34fcb45db |
| institution | Directory Open Access Journal |
| issn | 1664-2392 |
| language | English |
| last_indexed | 2024-04-10T07:06:50Z |
| publishDate | 2023-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Endocrinology |
| spelling | doaj.art-074f5fa952174212822f95a34fcb45db2023-02-27T06:07:34ZengFrontiers Media S.A.Frontiers in Endocrinology1664-23922023-02-011410.3389/fendo.2023.10874421087442Integrated analysis of long non-coding RNAs and mRNAs associated with glaucoma in vitroMengling You0Mengling You1Mengling You2Rong Rong3Rong Rong4Rong Rong5Zhou Zeng6Zhou Zeng7Zhou Zeng8Cong Fan9Cong Fan10Cong Fan11Haibo Li12Haibo Li13Haibo Li14Qian Yang15Qian Yang16Qian Yang17Dan Ji18Dan Ji19Dan Ji20Department of Ophthalmology, Xiangya Hospital, Central South University, Changsha, ChinaHunan Key Laboratory of Ophthalmology, Xiangya Hospital, Central South University, Changsha, ChinaNational Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, ChinaDepartment of Ophthalmology, Xiangya Hospital, Central South University, Changsha, ChinaHunan Key Laboratory of Ophthalmology, Xiangya Hospital, Central South University, Changsha, ChinaNational Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, ChinaDepartment of Ophthalmology, Xiangya Hospital, Central South University, Changsha, ChinaHunan Key Laboratory of Ophthalmology, Xiangya Hospital, Central South University, Changsha, ChinaNational Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, ChinaDepartment of Ophthalmology, Xiangya Hospital, Central South University, Changsha, ChinaHunan Key Laboratory of Ophthalmology, Xiangya Hospital, Central South University, Changsha, ChinaNational Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, ChinaDepartment of Ophthalmology, Xiangya Hospital, Central South University, Changsha, ChinaHunan Key Laboratory of Ophthalmology, Xiangya Hospital, Central South University, Changsha, ChinaNational Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, ChinaDepartment of Ophthalmology, Xiangya Hospital, Central South University, Changsha, ChinaHunan Key Laboratory of Ophthalmology, Xiangya Hospital, Central South University, Changsha, ChinaNational Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, ChinaDepartment of Ophthalmology, Xiangya Hospital, Central South University, Changsha, ChinaHunan Key Laboratory of Ophthalmology, Xiangya Hospital, Central South University, Changsha, ChinaNational Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, ChinaIntroductionIn recent years, the biological functions and important roles of long non-coding RNAs (lncRNAs) have been widely reported in many diseases. Although glaucoma is the leading cause of blindness worldwide, the specific mechanisms of lncRNAs in the pathogenesis and progression of glaucoma remain unclear. Our research aims to elucidate the differentially expressed lncRNAs and mRNAs in glaucoma and to provide a basis for further exploration of the specific mechanism of action of lncRNAs in the progression of glaucoma.MethodsWe performed RNA sequencing on samples from a pressurized model of R28 cells and performed bioinformatics analyses on the sequencing results. The expression consistency of lncRNAs in clinical samples from patients with glaucoma or cataracts was detected using real-time quantitative polymerase chain reaction (RT-qPCR).ResultsRNA sequencing results showed that lncRNAs in cluster 5 were upregulated with increasing stress after typing all significantly altered lncRNAs using k-means in a cellular stress model. KEGG analysis indicated that they were associated with neurodegenerative diseases. Differentially expressed lncRNAs were verified by RT-qPCR, and the lncRNA expression levels of AC120246.2 and XLOC_006247 were significantly higher in the aqueous humor (AH) of patients with glaucoma than in those with cataracts. For LOC102551819, there was almost no expression in the AH and trabecular meshwork in patients with glaucoma but high expression was observed in the iris. ConclusionOur research proposes potential diagnostic or intervention targets for clinical applications as well as a theoretical basis for more in-depth research on the function of lncRNAs in glaucoma.https://www.frontiersin.org/articles/10.3389/fendo.2023.1087442/fulllong non-coding RNAmRNAglaucomaco-expression networkcompetitive endogenous RNA |
| spellingShingle | Mengling You Mengling You Mengling You Rong Rong Rong Rong Rong Rong Zhou Zeng Zhou Zeng Zhou Zeng Cong Fan Cong Fan Cong Fan Haibo Li Haibo Li Haibo Li Qian Yang Qian Yang Qian Yang Dan Ji Dan Ji Dan Ji Integrated analysis of long non-coding RNAs and mRNAs associated with glaucoma in vitro Frontiers in Endocrinology long non-coding RNA mRNA glaucoma co-expression network competitive endogenous RNA |
| title | Integrated analysis of long non-coding RNAs and mRNAs associated with glaucoma in vitro |
| title_full | Integrated analysis of long non-coding RNAs and mRNAs associated with glaucoma in vitro |
| title_fullStr | Integrated analysis of long non-coding RNAs and mRNAs associated with glaucoma in vitro |
| title_full_unstemmed | Integrated analysis of long non-coding RNAs and mRNAs associated with glaucoma in vitro |
| title_short | Integrated analysis of long non-coding RNAs and mRNAs associated with glaucoma in vitro |
| title_sort | integrated analysis of long non coding rnas and mrnas associated with glaucoma in vitro |
| topic | long non-coding RNA mRNA glaucoma co-expression network competitive endogenous RNA |
| url | https://www.frontiersin.org/articles/10.3389/fendo.2023.1087442/full |
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