Biosorption of chromium by Pseudomonas sp. isolated from oil contaminated soils of Khuzestan
Introduction: In the last decade, biology and microorganisms is built to clean up environmental pollutants such as heavy metals. Isolation of metal resistant strains to achieve the goal is very important and the aim of this study was isolate the chrome resistant strains. ...
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| Format: | Article |
| Language: | English |
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University of Isfahan
2015-09-01
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| Series: | Biological Journal of Microorganism |
| Subjects: | |
| Online Access: | http://uijs.ui.ac.ir/bjm//browse.php?a_code=A-10-298-1&slc_lang=en&sid=1 |
| _version_ | 1817992341756051456 |
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| author | Seyed Mansour Meybodi Homa Khorasani Esmaili |
| author_facet | Seyed Mansour Meybodi Homa Khorasani Esmaili |
| author_sort | Seyed Mansour Meybodi |
| collection | DOAJ |
| description | Introduction: In the last decade,
biology and microorganisms is built to clean up environmental pollutants such
as heavy metals. Isolation of metal resistant
strains to achieve the goal is very important and the aim of this study was
isolate the chrome resistant strains. Materials and methods: Five
samples of oil contaminated soils of Khuzestan zones were collected under
sterile conditions and transferred to laboratory immediately. The soil samples
were homogenized and diluted by sterile saline up to 10-10 and
cultured on Luria Bertani agar containing 5ppm potassium dichromate. Resistant
strains were isolated after 24 hours of incubation, and then for isolation of
appropriate strains, cultured on Macconkey agar. Isolated bacteria were
identified by biochemical tests. Then, Luria Bertani agar was used for
screening of resistant strains in form of MIC test. The best conditions for
bacterial growth were found in the presence of chromium in various
temperatures, rate of shaking and pH values by spectrophotometry at 600 nm in
the overnight of cultivation. Absorption tests of metal were started under
optimal conditions. Results: From total of 24 strains of isolated Pseudomonads, 14
strains were resistant to chromium. The best strain (Mac-2) had removed
35.60 % of chromium from aqueous culture mediums under optimal conditions (pH:
8, Temp: 40°C and Shaking rate: 200rpm). Discussion and conclusion: Since heavy metals in various
physicochemical forms are considered as environmental pollutants in most parts
of the world, thus, the contaminated regions must be detoxified. The isolated
strain can be used for studies of bioremediation of chromium contaminated sites. |
| first_indexed | 2024-04-14T01:25:03Z |
| format | Article |
| id | doaj.art-074f681551864765841414328f29d8ff |
| institution | Directory Open Access Journal |
| issn | 2322-5173 2322-5181 |
| language | English |
| last_indexed | 2024-04-14T01:25:03Z |
| publishDate | 2015-09-01 |
| publisher | University of Isfahan |
| record_format | Article |
| series | Biological Journal of Microorganism |
| spelling | doaj.art-074f681551864765841414328f29d8ff2022-12-22T02:20:27ZengUniversity of IsfahanBiological Journal of Microorganism2322-51732322-51812015-09-01414101110Biosorption of chromium by Pseudomonas sp. isolated from oil contaminated soils of KhuzestanSeyed Mansour Meybodi0Homa Khorasani Esmaili1 Assistant Professor of Microbiology, Islamic Azad University, Tonekabon, Iran M.Sc. of Microbiology, Islamic Azad University, Tonekabon, Iran Introduction: In the last decade, biology and microorganisms is built to clean up environmental pollutants such as heavy metals. Isolation of metal resistant strains to achieve the goal is very important and the aim of this study was isolate the chrome resistant strains. Materials and methods: Five samples of oil contaminated soils of Khuzestan zones were collected under sterile conditions and transferred to laboratory immediately. The soil samples were homogenized and diluted by sterile saline up to 10-10 and cultured on Luria Bertani agar containing 5ppm potassium dichromate. Resistant strains were isolated after 24 hours of incubation, and then for isolation of appropriate strains, cultured on Macconkey agar. Isolated bacteria were identified by biochemical tests. Then, Luria Bertani agar was used for screening of resistant strains in form of MIC test. The best conditions for bacterial growth were found in the presence of chromium in various temperatures, rate of shaking and pH values by spectrophotometry at 600 nm in the overnight of cultivation. Absorption tests of metal were started under optimal conditions. Results: From total of 24 strains of isolated Pseudomonads, 14 strains were resistant to chromium. The best strain (Mac-2) had removed 35.60 % of chromium from aqueous culture mediums under optimal conditions (pH: 8, Temp: 40°C and Shaking rate: 200rpm). Discussion and conclusion: Since heavy metals in various physicochemical forms are considered as environmental pollutants in most parts of the world, thus, the contaminated regions must be detoxified. The isolated strain can be used for studies of bioremediation of chromium contaminated sites. http://uijs.ui.ac.ir/bjm//browse.php?a_code=A-10-298-1&slc_lang=en&sid=1Biosorption Chromium Pseudomonas Oil contaminated soil |
| spellingShingle | Seyed Mansour Meybodi Homa Khorasani Esmaili Biosorption of chromium by Pseudomonas sp. isolated from oil contaminated soils of Khuzestan Biological Journal of Microorganism Biosorption Chromium Pseudomonas Oil contaminated soil |
| title | Biosorption of chromium by Pseudomonas sp. isolated from oil contaminated soils of Khuzestan |
| title_full | Biosorption of chromium by Pseudomonas sp. isolated from oil contaminated soils of Khuzestan |
| title_fullStr | Biosorption of chromium by Pseudomonas sp. isolated from oil contaminated soils of Khuzestan |
| title_full_unstemmed | Biosorption of chromium by Pseudomonas sp. isolated from oil contaminated soils of Khuzestan |
| title_short | Biosorption of chromium by Pseudomonas sp. isolated from oil contaminated soils of Khuzestan |
| title_sort | biosorption of chromium by pseudomonas sp isolated from oil contaminated soils of khuzestan |
| topic | Biosorption Chromium Pseudomonas Oil contaminated soil |
| url | http://uijs.ui.ac.ir/bjm//browse.php?a_code=A-10-298-1&slc_lang=en&sid=1 |
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