Exploring Conditions for Handling Packing and Shipping <i>Aedes aegypti</i> Males to Support an SIT Field Project in Brazil
The sterile insect technique (SIT) application, as an alternative tool for conventional mosquito control methods, has recently gained prominence. Nevertheless, some SIT components require further development, such as protocols under large-scale conditions, focusing on packing and shipping mosquitoes...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-09-01
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| Series: | Insects |
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| Online Access: | https://www.mdpi.com/2075-4450/13/10/871 |
| _version_ | 1827649713061494784 |
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| author | Maylen Gómez Aline T. Macedo Michelle C. Pedrosa Fernanda Hohana Verenna Barros Bianca Pires Lucas Barbosa Miriam Brito Luiza Garziera Rafael Argilés-Herrero Jair F. Virginio Danilo O. Carvalho |
| author_facet | Maylen Gómez Aline T. Macedo Michelle C. Pedrosa Fernanda Hohana Verenna Barros Bianca Pires Lucas Barbosa Miriam Brito Luiza Garziera Rafael Argilés-Herrero Jair F. Virginio Danilo O. Carvalho |
| author_sort | Maylen Gómez |
| collection | DOAJ |
| description | The sterile insect technique (SIT) application, as an alternative tool for conventional mosquito control methods, has recently gained prominence. Nevertheless, some SIT components require further development, such as protocols under large-scale conditions, focusing on packing and shipping mosquitoes, and considering transporting time. Immobilization of <i>Aedes aegypti</i> males was tested at temperatures 4, 7, 10, and 14 °C, and each temperature was assessed for 60, 90, and 120 min. The recovery after 24 h was also studied. Chilled and control-reared males had comparable survival rates for all conditions, although 4 °C for 120 min impacted male survival. The male escape rate was affected after 60 min of exposure at 4 °C; this difference was not significant, with 24 h of recovery. First, we defined the successful immobilization at 4 °C for 60 min, thus enabling the evaluation of two transportation intervals: 6 and 24 h, with the assessment of different compaction densities of 100 and 150 mosquitoes/cm<sup>3</sup> at 10 °C to optimize the shipment. Compaction during simulated mosquito shipments reduced survival rates significantly after 6 and 24 h. In the mating propensity and insemination experiments, the sterile males managed to inseminate 40 to 66% for all treatments in laboratory conditions. The male insemination propensity was affected only by the highest compaction condition concerning the control. The analysis of the densities (100 and 150 males/cm<sup>3</sup>) showed that a higher density combined with an extended shipment period (24 h) negatively impacted the percentage of inseminated females. The results are very helpful in developing and improving the SIT packing and shipment protocols. Further studies are required to evaluate all combined parameters’ synergetic effects that can combine irradiation to assess sexual competitiveness when sterile males are released into the field. |
| first_indexed | 2024-03-09T20:03:29Z |
| format | Article |
| id | doaj.art-075133a2f3aa4d33beec8909159768d7 |
| institution | Directory Open Access Journal |
| issn | 2075-4450 |
| language | English |
| last_indexed | 2024-03-09T20:03:29Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Insects |
| spelling | doaj.art-075133a2f3aa4d33beec8909159768d72023-11-24T00:37:29ZengMDPI AGInsects2075-44502022-09-01131087110.3390/insects13100871Exploring Conditions for Handling Packing and Shipping <i>Aedes aegypti</i> Males to Support an SIT Field Project in BrazilMaylen Gómez0Aline T. Macedo1Michelle C. Pedrosa2Fernanda Hohana3Verenna Barros4Bianca Pires5Lucas Barbosa6Miriam Brito7Luiza Garziera8Rafael Argilés-Herrero9Jair F. Virginio10Danilo O. Carvalho11Biofábrica Moscamed Brasil, Quadra D-13, Lote 15, Distrito Industrial do São Francisco, Juazeiro 48909-733, BrazilBiofábrica Moscamed Brasil, Quadra D-13, Lote 15, Distrito Industrial do São Francisco, Juazeiro 48909-733, BrazilBiofábrica Moscamed Brasil, Quadra D-13, Lote 15, Distrito Industrial do São Francisco, Juazeiro 48909-733, BrazilBiofábrica Moscamed Brasil, Quadra D-13, Lote 15, Distrito Industrial do São Francisco, Juazeiro 48909-733, BrazilBiofábrica Moscamed Brasil, Quadra D-13, Lote 15, Distrito Industrial do São Francisco, Juazeiro 48909-733, BrazilBiofábrica Moscamed Brasil, Quadra D-13, Lote 15, Distrito Industrial do São Francisco, Juazeiro 48909-733, BrazilBiofábrica Moscamed Brasil, Quadra D-13, Lote 15, Distrito Industrial do São Francisco, Juazeiro 48909-733, BrazilBiofábrica Moscamed Brasil, Quadra D-13, Lote 15, Distrito Industrial do São Francisco, Juazeiro 48909-733, BrazilBiofábrica Moscamed Brasil, Quadra D-13, Lote 15, Distrito Industrial do São Francisco, Juazeiro 48909-733, BrazilInsect Pest Control Subprogramme, Joint FAO/IAEA Programme of Nuclear Techniques in Food and Agriculture, P.O. Box 100 Vienna, AustriaBiofábrica Moscamed Brasil, Quadra D-13, Lote 15, Distrito Industrial do São Francisco, Juazeiro 48909-733, BrazilInsect Pest Control Subprogramme, Joint FAO/IAEA Programme of Nuclear Techniques in Food and Agriculture, P.O. Box 100 Vienna, AustriaThe sterile insect technique (SIT) application, as an alternative tool for conventional mosquito control methods, has recently gained prominence. Nevertheless, some SIT components require further development, such as protocols under large-scale conditions, focusing on packing and shipping mosquitoes, and considering transporting time. Immobilization of <i>Aedes aegypti</i> males was tested at temperatures 4, 7, 10, and 14 °C, and each temperature was assessed for 60, 90, and 120 min. The recovery after 24 h was also studied. Chilled and control-reared males had comparable survival rates for all conditions, although 4 °C for 120 min impacted male survival. The male escape rate was affected after 60 min of exposure at 4 °C; this difference was not significant, with 24 h of recovery. First, we defined the successful immobilization at 4 °C for 60 min, thus enabling the evaluation of two transportation intervals: 6 and 24 h, with the assessment of different compaction densities of 100 and 150 mosquitoes/cm<sup>3</sup> at 10 °C to optimize the shipment. Compaction during simulated mosquito shipments reduced survival rates significantly after 6 and 24 h. In the mating propensity and insemination experiments, the sterile males managed to inseminate 40 to 66% for all treatments in laboratory conditions. The male insemination propensity was affected only by the highest compaction condition concerning the control. The analysis of the densities (100 and 150 males/cm<sup>3</sup>) showed that a higher density combined with an extended shipment period (24 h) negatively impacted the percentage of inseminated females. The results are very helpful in developing and improving the SIT packing and shipment protocols. Further studies are required to evaluate all combined parameters’ synergetic effects that can combine irradiation to assess sexual competitiveness when sterile males are released into the field.https://www.mdpi.com/2075-4450/13/10/871sterile mosquitoesshipmentknockoutpackaging densitieschillingquality control |
| spellingShingle | Maylen Gómez Aline T. Macedo Michelle C. Pedrosa Fernanda Hohana Verenna Barros Bianca Pires Lucas Barbosa Miriam Brito Luiza Garziera Rafael Argilés-Herrero Jair F. Virginio Danilo O. Carvalho Exploring Conditions for Handling Packing and Shipping <i>Aedes aegypti</i> Males to Support an SIT Field Project in Brazil Insects sterile mosquitoes shipment knockout packaging densities chilling quality control |
| title | Exploring Conditions for Handling Packing and Shipping <i>Aedes aegypti</i> Males to Support an SIT Field Project in Brazil |
| title_full | Exploring Conditions for Handling Packing and Shipping <i>Aedes aegypti</i> Males to Support an SIT Field Project in Brazil |
| title_fullStr | Exploring Conditions for Handling Packing and Shipping <i>Aedes aegypti</i> Males to Support an SIT Field Project in Brazil |
| title_full_unstemmed | Exploring Conditions for Handling Packing and Shipping <i>Aedes aegypti</i> Males to Support an SIT Field Project in Brazil |
| title_short | Exploring Conditions for Handling Packing and Shipping <i>Aedes aegypti</i> Males to Support an SIT Field Project in Brazil |
| title_sort | exploring conditions for handling packing and shipping i aedes aegypti i males to support an sit field project in brazil |
| topic | sterile mosquitoes shipment knockout packaging densities chilling quality control |
| url | https://www.mdpi.com/2075-4450/13/10/871 |
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