VIABILIDADE DE FOLÍCULOS PRÉ-ANTRAIS BOVINOS ISOLADOS DE OVÁRIOS APÓS VITRIFICAÇÃO
The aim of this study was to evaluate isolated bovine preantral follicles after vitrification procedure. Ovaries were obtained at a local slaughterhouse and submitted to follicular isolation. From the obtained suspension, one part was destined to control group. The remaining suspension was divided i...
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| Format: | Article |
| Language: | English |
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Universidade Federal de Goiás
2011-12-01
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| Series: | Ciência Animal Brasileira |
| Subjects: | |
| Online Access: | http://revistas.ufg.br/index.php/vet/article/view/3793/10067 |
| _version_ | 1818530650494337024 |
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| author | Hélder Silva Luna Viviane Bento Silva Fernanda Araujo Abreu |
| author_facet | Hélder Silva Luna Viviane Bento Silva Fernanda Araujo Abreu |
| author_sort | Hélder Silva Luna |
| collection | DOAJ |
| description | The aim of this study was to evaluate isolated bovine preantral follicles after vitrification procedure. Ovaries were obtained at a local slaughterhouse and submitted to follicular isolation. From the obtained suspension, one part was destined to control group. The remaining suspension was divided into two parts, one to the toxicity test and the other one to the vitrification procedure. For vitrification, the follicles were exposed for 1 min to vitrification solution-1, composed of 10% ethylene glycol, 10% DMSO in saline solution (NaCl 0.9%), and then 1 min to vitrification solution-2, composed of 20% ethylene glycol, 20% DMSO in the same saline solution supplemented with 0.5 M sucrose. The follicles were vitrified by use of glass capillaries and remained vitrified for 24 h. After warming, they were transferred to a 0.25 M sucrose solution for 5 min and then transferred to a 0.15 M sucrose solution for another 5 min. For the toxicity test, the follicles were only exposed to cryoprotectants and analised. The preantral follicles were classified as non-viable orviable when they were stained or not stained by trypan blue, respectively. Differences (P<0.05) in follicular viability were observed among the control, non-vitrified group (93.6%), and exposed (64.1%) or vitrified follicles (66%). The percentages of normal follicles found after exposure to cryoprotectant did not differ significantly (P>0.05) from that found after vitrification, indicating that the reduction of percentage of viable preantral follicles was due to a toxic effect of cryoprotectants and not to the vitrification procedure itself. |
| first_indexed | 2024-12-11T17:22:23Z |
| format | Article |
| id | doaj.art-075281b0023a462d9d093d2c05950fd0 |
| institution | Directory Open Access Journal |
| issn | 1518-2797 1089-6891 |
| language | English |
| last_indexed | 2024-12-11T17:22:23Z |
| publishDate | 2011-12-01 |
| publisher | Universidade Federal de Goiás |
| record_format | Article |
| series | Ciência Animal Brasileira |
| spelling | doaj.art-075281b0023a462d9d093d2c05950fd02022-12-22T00:57:04ZengUniversidade Federal de GoiásCiência Animal Brasileira1518-27971089-68912011-12-01124693698VIABILIDADE DE FOLÍCULOS PRÉ-ANTRAIS BOVINOS ISOLADOS DE OVÁRIOS APÓS VITRIFICAÇÃOHélder Silva LunaViviane Bento SilvaFernanda Araujo AbreuThe aim of this study was to evaluate isolated bovine preantral follicles after vitrification procedure. Ovaries were obtained at a local slaughterhouse and submitted to follicular isolation. From the obtained suspension, one part was destined to control group. The remaining suspension was divided into two parts, one to the toxicity test and the other one to the vitrification procedure. For vitrification, the follicles were exposed for 1 min to vitrification solution-1, composed of 10% ethylene glycol, 10% DMSO in saline solution (NaCl 0.9%), and then 1 min to vitrification solution-2, composed of 20% ethylene glycol, 20% DMSO in the same saline solution supplemented with 0.5 M sucrose. The follicles were vitrified by use of glass capillaries and remained vitrified for 24 h. After warming, they were transferred to a 0.25 M sucrose solution for 5 min and then transferred to a 0.15 M sucrose solution for another 5 min. For the toxicity test, the follicles were only exposed to cryoprotectants and analised. The preantral follicles were classified as non-viable orviable when they were stained or not stained by trypan blue, respectively. Differences (P<0.05) in follicular viability were observed among the control, non-vitrified group (93.6%), and exposed (64.1%) or vitrified follicles (66%). The percentages of normal follicles found after exposure to cryoprotectant did not differ significantly (P>0.05) from that found after vitrification, indicating that the reduction of percentage of viable preantral follicles was due to a toxic effect of cryoprotectants and not to the vitrification procedure itself.http://revistas.ufg.br/index.php/vet/article/view/3793/10067bovinecryoprotectantsoocytesvitrification. |
| spellingShingle | Hélder Silva Luna Viviane Bento Silva Fernanda Araujo Abreu VIABILIDADE DE FOLÍCULOS PRÉ-ANTRAIS BOVINOS ISOLADOS DE OVÁRIOS APÓS VITRIFICAÇÃO Ciência Animal Brasileira bovine cryoprotectants oocytes vitrification. |
| title | VIABILIDADE DE FOLÍCULOS PRÉ-ANTRAIS BOVINOS ISOLADOS DE OVÁRIOS APÓS VITRIFICAÇÃO |
| title_full | VIABILIDADE DE FOLÍCULOS PRÉ-ANTRAIS BOVINOS ISOLADOS DE OVÁRIOS APÓS VITRIFICAÇÃO |
| title_fullStr | VIABILIDADE DE FOLÍCULOS PRÉ-ANTRAIS BOVINOS ISOLADOS DE OVÁRIOS APÓS VITRIFICAÇÃO |
| title_full_unstemmed | VIABILIDADE DE FOLÍCULOS PRÉ-ANTRAIS BOVINOS ISOLADOS DE OVÁRIOS APÓS VITRIFICAÇÃO |
| title_short | VIABILIDADE DE FOLÍCULOS PRÉ-ANTRAIS BOVINOS ISOLADOS DE OVÁRIOS APÓS VITRIFICAÇÃO |
| title_sort | viabilidade de foliculos pre antrais bovinos isolados de ovarios apos vitrificacao |
| topic | bovine cryoprotectants oocytes vitrification. |
| url | http://revistas.ufg.br/index.php/vet/article/view/3793/10067 |
| work_keys_str_mv | AT heldersilvaluna viabilidadedefoliculospreantraisbovinosisoladosdeovariosaposvitrificacao AT vivianebentosilva viabilidadedefoliculospreantraisbovinosisoladosdeovariosaposvitrificacao AT fernandaaraujoabreu viabilidadedefoliculospreantraisbovinosisoladosdeovariosaposvitrificacao |