Composition, taxonomy and functional diversity of the oropharynx microbiome in individuals with schizophrenia and controls
The role of the human microbiome in schizophrenia remains largely unexplored. The microbiome has been shown to alter brain development and modulate behavior and cognition in animals through gut-brain connections, and research in humans suggests that it may be a modulating factor in many disorders. T...
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| Format: | Article |
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PeerJ Inc.
2015-08-01
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| Online Access: | https://peerj.com/articles/1140.pdf |
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| author | Eduardo Castro-Nallar Matthew L. Bendall Marcos Pérez-Losada Sarven Sabuncyan Emily G. Severance Faith B. Dickerson Jennifer R. Schroeder Robert H. Yolken Keith A. Crandall |
| author_facet | Eduardo Castro-Nallar Matthew L. Bendall Marcos Pérez-Losada Sarven Sabuncyan Emily G. Severance Faith B. Dickerson Jennifer R. Schroeder Robert H. Yolken Keith A. Crandall |
| author_sort | Eduardo Castro-Nallar |
| collection | DOAJ |
| description | The role of the human microbiome in schizophrenia remains largely unexplored. The microbiome has been shown to alter brain development and modulate behavior and cognition in animals through gut-brain connections, and research in humans suggests that it may be a modulating factor in many disorders. This study reports findings from a shotgun metagenomic analysis of the oropharyngeal microbiome in 16 individuals with schizophrenia and 16 controls. High-level differences were evident at both the phylum and genus levels, with Proteobacteria, Firmicutes, Bacteroidetes, and Actinobacteria dominating both schizophrenia patients and controls, and Ascomycota being more abundant in schizophrenia patients than controls. Controls were richer in species but less even in their distributions, i.e., dominated by fewer species, as opposed to schizophrenia patients. Lactic acid bacteria were relatively more abundant in schizophrenia, including species of Lactobacilli and Bifidobacterium, which have been shown to modulate chronic inflammation. We also found Eubacterium halii, a lactate-utilizing species. Functionally, the microbiome of schizophrenia patients was characterized by an increased number of metabolic pathways related to metabolite transport systems including siderophores, glutamate, and vitamin B12. In contrast, carbohydrate and lipid pathways and energy metabolism were abundant in controls. These findings suggest that the oropharyngeal microbiome in individuals with schizophrenia is significantly different compared to controls, and that particular microbial species and metabolic pathways differentiate both groups. Confirmation of these findings in larger and more diverse samples, e.g., gut microbiome, will contribute to elucidating potential links between schizophrenia and the human microbiota. |
| first_indexed | 2024-03-09T06:50:48Z |
| format | Article |
| id | doaj.art-0753dfc1e1ba42e7a4e63d36c5960354 |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T06:50:48Z |
| publishDate | 2015-08-01 |
| publisher | PeerJ Inc. |
| record_format | Article |
| series | PeerJ |
| spelling | doaj.art-0753dfc1e1ba42e7a4e63d36c59603542023-12-03T10:26:50ZengPeerJ Inc.PeerJ2167-83592015-08-013e114010.7717/peerj.1140Composition, taxonomy and functional diversity of the oropharynx microbiome in individuals with schizophrenia and controlsEduardo Castro-Nallar0Matthew L. Bendall1Marcos Pérez-Losada2Sarven Sabuncyan3Emily G. Severance4Faith B. Dickerson5Jennifer R. Schroeder6Robert H. Yolken7Keith A. Crandall8Computational Biology Institute, George Washington University, Ashburn, VA, USAComputational Biology Institute, George Washington University, Ashburn, VA, USAComputational Biology Institute, George Washington University, Ashburn, VA, USAStanley Neurovirology Laboratory, Johns Hopkins School of Medicine, Baltimore, MD, USAStanley Neurovirology Laboratory, Johns Hopkins School of Medicine, Baltimore, MD, USASheppard Pratt Hospital, Baltimore, MD, USASchroeder Statistical Consulting LLC, Ellicott City, MD, USAStanley Neurovirology Laboratory, Johns Hopkins School of Medicine, Baltimore, MD, USAComputational Biology Institute, George Washington University, Ashburn, VA, USAThe role of the human microbiome in schizophrenia remains largely unexplored. The microbiome has been shown to alter brain development and modulate behavior and cognition in animals through gut-brain connections, and research in humans suggests that it may be a modulating factor in many disorders. This study reports findings from a shotgun metagenomic analysis of the oropharyngeal microbiome in 16 individuals with schizophrenia and 16 controls. High-level differences were evident at both the phylum and genus levels, with Proteobacteria, Firmicutes, Bacteroidetes, and Actinobacteria dominating both schizophrenia patients and controls, and Ascomycota being more abundant in schizophrenia patients than controls. Controls were richer in species but less even in their distributions, i.e., dominated by fewer species, as opposed to schizophrenia patients. Lactic acid bacteria were relatively more abundant in schizophrenia, including species of Lactobacilli and Bifidobacterium, which have been shown to modulate chronic inflammation. We also found Eubacterium halii, a lactate-utilizing species. Functionally, the microbiome of schizophrenia patients was characterized by an increased number of metabolic pathways related to metabolite transport systems including siderophores, glutamate, and vitamin B12. In contrast, carbohydrate and lipid pathways and energy metabolism were abundant in controls. These findings suggest that the oropharyngeal microbiome in individuals with schizophrenia is significantly different compared to controls, and that particular microbial species and metabolic pathways differentiate both groups. Confirmation of these findings in larger and more diverse samples, e.g., gut microbiome, will contribute to elucidating potential links between schizophrenia and the human microbiota.https://peerj.com/articles/1140.pdfMetagenomicsMicrobiomeShotgun sequencingSchizophreniaMicrobial diversityLactic acid bacteria |
| spellingShingle | Eduardo Castro-Nallar Matthew L. Bendall Marcos Pérez-Losada Sarven Sabuncyan Emily G. Severance Faith B. Dickerson Jennifer R. Schroeder Robert H. Yolken Keith A. Crandall Composition, taxonomy and functional diversity of the oropharynx microbiome in individuals with schizophrenia and controls PeerJ Metagenomics Microbiome Shotgun sequencing Schizophrenia Microbial diversity Lactic acid bacteria |
| title | Composition, taxonomy and functional diversity of the oropharynx microbiome in individuals with schizophrenia and controls |
| title_full | Composition, taxonomy and functional diversity of the oropharynx microbiome in individuals with schizophrenia and controls |
| title_fullStr | Composition, taxonomy and functional diversity of the oropharynx microbiome in individuals with schizophrenia and controls |
| title_full_unstemmed | Composition, taxonomy and functional diversity of the oropharynx microbiome in individuals with schizophrenia and controls |
| title_short | Composition, taxonomy and functional diversity of the oropharynx microbiome in individuals with schizophrenia and controls |
| title_sort | composition taxonomy and functional diversity of the oropharynx microbiome in individuals with schizophrenia and controls |
| topic | Metagenomics Microbiome Shotgun sequencing Schizophrenia Microbial diversity Lactic acid bacteria |
| url | https://peerj.com/articles/1140.pdf |
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