The effect of Chitosan on antioxidant activity and some secondary metabolites of Mentha piperita L.
Plants are almost reach sources of phenolic compounds such as flavonoids which are the most important natural antioxidants. Antioxidant compounds are essential for protecting human body against oxidative stress. However use elicitors could increase the antioxidant activity of plant. The...
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| Format: | Article |
| Language: | English |
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Islamic Azad University
2016-09-01
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| Series: | Journal of Pharmaceutical and Health Sciences |
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| Online Access: | http://www.jphs.ir/article_527130.html |
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| author | Sima Salimgandomi Azita Shabrangi |
| author_facet | Sima Salimgandomi Azita Shabrangi |
| author_sort | Sima Salimgandomi |
| collection | DOAJ |
| description | Plants are almost reach sources of phenolic compounds such as flavonoids which are the most important natural antioxidants. Antioxidant compounds are essential for protecting human body against oxidative stress. However use elicitors could increase the antioxidant activity of plant. The purpose of this study
was to evaluate the effects of Chitosan on the content of phenolic and flavonoid compounds and also antioxidant activity Mentha piperita L.In this study, firstly the Mentha piperita L. were grown up for 6-week period at greenhouse conditions and then were treated with 50-100 μm of chitosan, then total phenolic and flavonoid contents were determined using spectrophotometry and finally antioxidant activities of extracts were evaluated with 2,2-diphenyl-1-picryl hydrazyl (DPPH) method and the results were analyzed with Excel software and Variance analysis testing method with SPSS software. The results showed the content of phenolic compounds in the methanol extract based on sample mg of Gallic acid /g for control with water, 50μm and 100μm treatments respectively were 146.8,233.1,339.1. Meanwhile the total volume
of flavonoid content of the methanol extract per mg of Rutin/g respectively were 9.88,12.11,14.06 and concentration of the said extracts respectively were 196.3,147.7,128.62. In regard to the above results it can be concluded that due to having phenolic and flavonoid contents, Mentha piperita L showed that antioxidant activity could be stimulated upon Chitosan treatment moreover, antioxidant activity increased by increasing Chitosan treatment content. Therefore, this method can be used to increase antioxidant effect of plant as a natural antioxidant and all the phenolic and flavonoid contents. |
| first_indexed | 2024-04-13T14:08:23Z |
| format | Article |
| id | doaj.art-075677cd1d124ee9b40adb87dcb6b558 |
| institution | Directory Open Access Journal |
| issn | 2228-6780 2322-4738 |
| language | English |
| last_indexed | 2024-04-13T14:08:23Z |
| publishDate | 2016-09-01 |
| publisher | Islamic Azad University |
| record_format | Article |
| series | Journal of Pharmaceutical and Health Sciences |
| spelling | doaj.art-075677cd1d124ee9b40adb87dcb6b5582022-12-22T02:43:51ZengIslamic Azad UniversityJournal of Pharmaceutical and Health Sciences2228-67802322-47382016-09-0142135142The effect of Chitosan on antioxidant activity and some secondary metabolites of Mentha piperita L.Sima Salimgandomi0Azita Shabrangi1Department of Medicinal Chemistry, Islamic Azad University-Pharmaceutical Sciences Branch (IAUPS), Tehran, Iran.Department of Medicinal Chemistry, Islamic Azad University-Pharmaceutical Sciences Branch (IAUPS), Tehran, Iran.Plants are almost reach sources of phenolic compounds such as flavonoids which are the most important natural antioxidants. Antioxidant compounds are essential for protecting human body against oxidative stress. However use elicitors could increase the antioxidant activity of plant. The purpose of this study was to evaluate the effects of Chitosan on the content of phenolic and flavonoid compounds and also antioxidant activity Mentha piperita L.In this study, firstly the Mentha piperita L. were grown up for 6-week period at greenhouse conditions and then were treated with 50-100 μm of chitosan, then total phenolic and flavonoid contents were determined using spectrophotometry and finally antioxidant activities of extracts were evaluated with 2,2-diphenyl-1-picryl hydrazyl (DPPH) method and the results were analyzed with Excel software and Variance analysis testing method with SPSS software. The results showed the content of phenolic compounds in the methanol extract based on sample mg of Gallic acid /g for control with water, 50μm and 100μm treatments respectively were 146.8,233.1,339.1. Meanwhile the total volume of flavonoid content of the methanol extract per mg of Rutin/g respectively were 9.88,12.11,14.06 and concentration of the said extracts respectively were 196.3,147.7,128.62. In regard to the above results it can be concluded that due to having phenolic and flavonoid contents, Mentha piperita L showed that antioxidant activity could be stimulated upon Chitosan treatment moreover, antioxidant activity increased by increasing Chitosan treatment content. Therefore, this method can be used to increase antioxidant effect of plant as a natural antioxidant and all the phenolic and flavonoid contents.http://www.jphs.ir/article_527130.htmlMentha piperita L.AntioxidantChitosanPhenolic contentFlavonoid content |
| spellingShingle | Sima Salimgandomi Azita Shabrangi The effect of Chitosan on antioxidant activity and some secondary metabolites of Mentha piperita L. Journal of Pharmaceutical and Health Sciences Mentha piperita L. Antioxidant Chitosan Phenolic content Flavonoid content |
| title | The effect of Chitosan on antioxidant activity and some secondary metabolites of Mentha piperita L. |
| title_full | The effect of Chitosan on antioxidant activity and some secondary metabolites of Mentha piperita L. |
| title_fullStr | The effect of Chitosan on antioxidant activity and some secondary metabolites of Mentha piperita L. |
| title_full_unstemmed | The effect of Chitosan on antioxidant activity and some secondary metabolites of Mentha piperita L. |
| title_short | The effect of Chitosan on antioxidant activity and some secondary metabolites of Mentha piperita L. |
| title_sort | effect of chitosan on antioxidant activity and some secondary metabolites of mentha piperita l |
| topic | Mentha piperita L. Antioxidant Chitosan Phenolic content Flavonoid content |
| url | http://www.jphs.ir/article_527130.html |
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