CD45RA and CD45RC isoforms expression in weaned pigs vaccinated with non-enterotoxigenic F4ac+ Escherichia coli strain against colibacillosis
Since no effective vaccine is available for its immunoprophylaxis, porcine post-weaning diarrhoea (PWD) induced by enterotoxigenic Escherichia coli (ETEC) strains remains an important cause of morbidity. Live attenuated oral vaccines have been suggested to be relatively effective in preventing ETEC-...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Czech Academy of Agricultural Sciences
2002-01-01
|
| Series: | Veterinární Medicína |
| Subjects: | |
| Online Access: | https://vetmed.agriculturejournals.cz/artkey/vet-200201-0002_cd45ra-and-cd45rc-isoforms-expression-in-weaned-pigs-vaccinated-with-non-enterotoxigenic-f4ac-escherichia-coli.php |
| _version_ | 1797897772984172544 |
|---|---|
| author | F. Božič L. Švec I. Valpotič |
| author_facet | F. Božič L. Švec I. Valpotič |
| author_sort | F. Božič |
| collection | DOAJ |
| description | Since no effective vaccine is available for its immunoprophylaxis, porcine post-weaning diarrhoea (PWD) induced by enterotoxigenic Escherichia coli (ETEC) strains remains an important cause of morbidity. Live attenuated oral vaccines have been suggested to be relatively effective in preventing ETEC-induced PWD in the pigs, but the mechanisms responsible for protection have not been elucidated. In the present study we have investigated the likely impact of oral vaccination of weaned pigs with non-ETEC strain expressing F4ac antigen on CD45RA and CD45RC isoforms expression on the surface of the mesenteric lymph node (MLN) cells and on spleen cells by using one-colour flow cytometry. Additionally, to assess the activation state of T cells in the MLN and spleen of the pigs, surface expression of the lymphocyte activation marker CD25 was analysed. Sham-vaccinated weaned pigs served as controls. Our results of the quantitative phenotypic analysis of isolated lymphocytes showed that the vaccinal E. coli strain induced elevation of both CD25+ and CD45RC+ cells in the spleen, but not MLN, of the vaccinated weaned pigs. This was accompanied by decreased CD45RA expression on spleen cells, suggesting that CD45RA+ spleen cells could develop CD45RC phenotype, probably as a consequence of activation in the vaccinated challenge-infected weaned pigs. |
| first_indexed | 2024-04-10T08:02:33Z |
| format | Article |
| id | doaj.art-075d682db3a64010b7286ca367f1ee1c |
| institution | Directory Open Access Journal |
| issn | 0375-8427 1805-9392 |
| language | English |
| last_indexed | 2024-04-10T08:02:33Z |
| publishDate | 2002-01-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Veterinární Medicína |
| spelling | doaj.art-075d682db3a64010b7286ca367f1ee1c2023-02-23T03:48:59ZengCzech Academy of Agricultural SciencesVeterinární Medicína0375-84271805-93922002-01-0147151110.17221/5795-VETMEDvet-200201-0002CD45RA and CD45RC isoforms expression in weaned pigs vaccinated with non-enterotoxigenic F4ac+ Escherichia coli strain against colibacillosisF. Božič0L. Švec1I. Valpotič2Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, University of Zagreb, CroatiaDepartment of Pharmacology and Toxicology, Faculty of Veterinary Medicine, University of Zagreb, CroatiaDepartment of Pharmacology and Toxicology, Faculty of Veterinary Medicine, University of Zagreb, CroatiaSince no effective vaccine is available for its immunoprophylaxis, porcine post-weaning diarrhoea (PWD) induced by enterotoxigenic Escherichia coli (ETEC) strains remains an important cause of morbidity. Live attenuated oral vaccines have been suggested to be relatively effective in preventing ETEC-induced PWD in the pigs, but the mechanisms responsible for protection have not been elucidated. In the present study we have investigated the likely impact of oral vaccination of weaned pigs with non-ETEC strain expressing F4ac antigen on CD45RA and CD45RC isoforms expression on the surface of the mesenteric lymph node (MLN) cells and on spleen cells by using one-colour flow cytometry. Additionally, to assess the activation state of T cells in the MLN and spleen of the pigs, surface expression of the lymphocyte activation marker CD25 was analysed. Sham-vaccinated weaned pigs served as controls. Our results of the quantitative phenotypic analysis of isolated lymphocytes showed that the vaccinal E. coli strain induced elevation of both CD25+ and CD45RC+ cells in the spleen, but not MLN, of the vaccinated weaned pigs. This was accompanied by decreased CD45RA expression on spleen cells, suggesting that CD45RA+ spleen cells could develop CD45RC phenotype, probably as a consequence of activation in the vaccinated challenge-infected weaned pigs.https://vetmed.agriculturejournals.cz/artkey/vet-200201-0002_cd45ra-and-cd45rc-isoforms-expression-in-weaned-pigs-vaccinated-with-non-enterotoxigenic-f4ac-escherichia-coli.phpweaned pigsvaccinationcolibacillosiscd45 isoformscd25flow cytom |
| spellingShingle | F. Božič L. Švec I. Valpotič CD45RA and CD45RC isoforms expression in weaned pigs vaccinated with non-enterotoxigenic F4ac+ Escherichia coli strain against colibacillosis Veterinární Medicína weaned pigs vaccination colibacillosis cd45 isoforms cd25 flow cytom |
| title | CD45RA and CD45RC isoforms expression in weaned pigs vaccinated with non-enterotoxigenic F4ac+ Escherichia coli strain against colibacillosis |
| title_full | CD45RA and CD45RC isoforms expression in weaned pigs vaccinated with non-enterotoxigenic F4ac+ Escherichia coli strain against colibacillosis |
| title_fullStr | CD45RA and CD45RC isoforms expression in weaned pigs vaccinated with non-enterotoxigenic F4ac+ Escherichia coli strain against colibacillosis |
| title_full_unstemmed | CD45RA and CD45RC isoforms expression in weaned pigs vaccinated with non-enterotoxigenic F4ac+ Escherichia coli strain against colibacillosis |
| title_short | CD45RA and CD45RC isoforms expression in weaned pigs vaccinated with non-enterotoxigenic F4ac+ Escherichia coli strain against colibacillosis |
| title_sort | cd45ra and cd45rc isoforms expression in weaned pigs vaccinated with non enterotoxigenic f4ac escherichia coli strain against colibacillosis |
| topic | weaned pigs vaccination colibacillosis cd45 isoforms cd25 flow cytom |
| url | https://vetmed.agriculturejournals.cz/artkey/vet-200201-0002_cd45ra-and-cd45rc-isoforms-expression-in-weaned-pigs-vaccinated-with-non-enterotoxigenic-f4ac-escherichia-coli.php |
| work_keys_str_mv | AT fbozic cd45raandcd45rcisoformsexpressioninweanedpigsvaccinatedwithnonenterotoxigenicf4acescherichiacolistrainagainstcolibacillosis AT lsvec cd45raandcd45rcisoformsexpressioninweanedpigsvaccinatedwithnonenterotoxigenicf4acescherichiacolistrainagainstcolibacillosis AT ivalpotic cd45raandcd45rcisoformsexpressioninweanedpigsvaccinatedwithnonenterotoxigenicf4acescherichiacolistrainagainstcolibacillosis |