Essential Oil Variability of Azorean <i>Cryptomeria japonica</i> Leaves under Different Distillation Methods, Part 2: Molluscicidal Activity and Brine Shrimp Lethality
This study reports on the in vivo molluscicidal activity and <i>Artemia salina</i> lethality of Azorean <i>Cryptomeria japonica</i> leaf (CJL) essential oils (EOs) obtained by hydrodistillation (HD) and water-steam distillation (WSD) techniques, especially in light of the rec...
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MDPI AG
2023-04-01
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| Online Access: | https://www.mdpi.com/2297-8739/10/4/241 |
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| author | Filipe Arruda Ana Lima Luísa Oliveira Tânia Rodrigues Alexandre Janeiro José S. Rosa Elisabete Lima |
| author_facet | Filipe Arruda Ana Lima Luísa Oliveira Tânia Rodrigues Alexandre Janeiro José S. Rosa Elisabete Lima |
| author_sort | Filipe Arruda |
| collection | DOAJ |
| description | This study reports on the in vivo molluscicidal activity and <i>Artemia salina</i> lethality of Azorean <i>Cryptomeria japonica</i> leaf (CJL) essential oils (EOs) obtained by hydrodistillation (HD) and water-steam distillation (WSD) techniques, especially in light of the recent focus on the use of forestry and wood industry residues in a sustainable world economy. Molluscicidal activity was performed during several life stages of <i>Radix peregra</i> by the immersion method, under laboratory conditions. A first screening through a single-dose bioassay revealed that both EOs were highly active towards eggs, juveniles and adult snails (ca. 100% mortality). Concentration- and time-toxicity assays were carried out only on adult snails to determine the lethal parameters (LC<sub>50;90</sub> and LT<sub>50;90</sub>). The LC<sub>50</sub> values were 33 and 62 µg/mL for EO–WSD and EO–HD, respectively, after 48 h. The LT<sub>50</sub> of EO–WSD required only 21 h for both 16 h and continous exposure periods, while that of EO–HD was slighty superior (21.8–25.6 h). Although not significant, EO–WSD was also slightly more toxic against <i>A. salina</i> than EO–HD (LC<sub>50</sub> = 98 and 115 µg/mL after 24 h, respectively). In conclusion, Azorean CJL EOs, which are rich in α-pinene (mostly EO–WSD), have huge potential to be used as safe raw materials for the development of natural molluscicide products to control snails responsible for transmitting fascioliasis. |
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| last_indexed | 2024-03-11T04:31:38Z |
| publishDate | 2023-04-01 |
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| spelling | doaj.art-07601a11910a47e188ac1e19fde685842023-11-17T21:20:28ZengMDPI AGSeparations2297-87392023-04-0110424110.3390/separations10040241Essential Oil Variability of Azorean <i>Cryptomeria japonica</i> Leaves under Different Distillation Methods, Part 2: Molluscicidal Activity and Brine Shrimp LethalityFilipe Arruda0Ana Lima1Luísa Oliveira2Tânia Rodrigues3Alexandre Janeiro4José S. Rosa5Elisabete Lima6Institute of Agricultural and Environmental Research and Technology (IITAA), University of the Azores, 9700-042 Angra do Heroísmo, PortugalInstitute of Agricultural and Environmental Research and Technology (IITAA), University of the Azores, 9700-042 Angra do Heroísmo, PortugalDepartment of Biology (DB), Faculty of Science and Technology, University of the Azores, 9500-321 Ponta Delgada, PortugalDepartment of Biology (DB), Faculty of Science and Technology, University of the Azores, 9500-321 Ponta Delgada, PortugalInstitute of Agricultural and Environmental Research and Technology (IITAA), University of the Azores, 9700-042 Angra do Heroísmo, PortugalDepartment of Biology (DB), Faculty of Science and Technology, University of the Azores, 9500-321 Ponta Delgada, PortugalInstitute of Agricultural and Environmental Research and Technology (IITAA), University of the Azores, 9700-042 Angra do Heroísmo, PortugalThis study reports on the in vivo molluscicidal activity and <i>Artemia salina</i> lethality of Azorean <i>Cryptomeria japonica</i> leaf (CJL) essential oils (EOs) obtained by hydrodistillation (HD) and water-steam distillation (WSD) techniques, especially in light of the recent focus on the use of forestry and wood industry residues in a sustainable world economy. Molluscicidal activity was performed during several life stages of <i>Radix peregra</i> by the immersion method, under laboratory conditions. A first screening through a single-dose bioassay revealed that both EOs were highly active towards eggs, juveniles and adult snails (ca. 100% mortality). Concentration- and time-toxicity assays were carried out only on adult snails to determine the lethal parameters (LC<sub>50;90</sub> and LT<sub>50;90</sub>). The LC<sub>50</sub> values were 33 and 62 µg/mL for EO–WSD and EO–HD, respectively, after 48 h. The LT<sub>50</sub> of EO–WSD required only 21 h for both 16 h and continous exposure periods, while that of EO–HD was slighty superior (21.8–25.6 h). Although not significant, EO–WSD was also slightly more toxic against <i>A. salina</i> than EO–HD (LC<sub>50</sub> = 98 and 115 µg/mL after 24 h, respectively). In conclusion, Azorean CJL EOs, which are rich in α-pinene (mostly EO–WSD), have huge potential to be used as safe raw materials for the development of natural molluscicide products to control snails responsible for transmitting fascioliasis.https://www.mdpi.com/2297-8739/10/4/241Azores<i>Cryptomeria japonica</i>biomass residues valorizationhydrodistillation and water-steam distillationessential oilbotanical pesticides |
| spellingShingle | Filipe Arruda Ana Lima Luísa Oliveira Tânia Rodrigues Alexandre Janeiro José S. Rosa Elisabete Lima Essential Oil Variability of Azorean <i>Cryptomeria japonica</i> Leaves under Different Distillation Methods, Part 2: Molluscicidal Activity and Brine Shrimp Lethality Separations Azores <i>Cryptomeria japonica</i> biomass residues valorization hydrodistillation and water-steam distillation essential oil botanical pesticides |
| title | Essential Oil Variability of Azorean <i>Cryptomeria japonica</i> Leaves under Different Distillation Methods, Part 2: Molluscicidal Activity and Brine Shrimp Lethality |
| title_full | Essential Oil Variability of Azorean <i>Cryptomeria japonica</i> Leaves under Different Distillation Methods, Part 2: Molluscicidal Activity and Brine Shrimp Lethality |
| title_fullStr | Essential Oil Variability of Azorean <i>Cryptomeria japonica</i> Leaves under Different Distillation Methods, Part 2: Molluscicidal Activity and Brine Shrimp Lethality |
| title_full_unstemmed | Essential Oil Variability of Azorean <i>Cryptomeria japonica</i> Leaves under Different Distillation Methods, Part 2: Molluscicidal Activity and Brine Shrimp Lethality |
| title_short | Essential Oil Variability of Azorean <i>Cryptomeria japonica</i> Leaves under Different Distillation Methods, Part 2: Molluscicidal Activity and Brine Shrimp Lethality |
| title_sort | essential oil variability of azorean i cryptomeria japonica i leaves under different distillation methods part 2 molluscicidal activity and brine shrimp lethality |
| topic | Azores <i>Cryptomeria japonica</i> biomass residues valorization hydrodistillation and water-steam distillation essential oil botanical pesticides |
| url | https://www.mdpi.com/2297-8739/10/4/241 |
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