The effect of the disulfideisomerase domain containing protein in the defense against polyhexamethylene biguanide of highly tolerant Acanthamoeba at the trophozoite stage
Acanthamoeba castellanii is a free-living protozoan pathogen capable of causing a blinding keratitis and fatal granulomatous encephalitis. Current treatment generally involves an hourly application of polyhexamethylene biguanide (PHMB) over a period of several days but this is not entirely effective...
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| Format: | Article |
| Language: | English |
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Elsevier
2016-12-01
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| Series: | International Journal for Parasitology: Drugs and Drug Resistance |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211320716300768 |
| _version_ | 1818060628661633024 |
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| author | Fu-Chin Huang Tao-Shen Liu Sung-Chou Li Min-Hsiu Shih Jyh-Wei Shin Wei-Chen Lin |
| author_facet | Fu-Chin Huang Tao-Shen Liu Sung-Chou Li Min-Hsiu Shih Jyh-Wei Shin Wei-Chen Lin |
| author_sort | Fu-Chin Huang |
| collection | DOAJ |
| description | Acanthamoeba castellanii is a free-living protozoan pathogen capable of causing a blinding keratitis and fatal granulomatous encephalitis. Current treatment generally involves an hourly application of polyhexamethylene biguanide (PHMB) over a period of several days but this is not entirely effective against all strains/isolates. The tolerance mechanisms of PHMB in Acanthamoeba cells remain unclear. In this study, we found that the mRNA expression level of disulfideisomerase domain containing protein (PDI) increased rapidly in surviving cells of the highly PHMB-tolerant Acanthamoeba castellanii strain, NCKH_D, during PHMB treatment, but not in the ATCC standard strain. After PDI-specific silencing, NCKH_D was found to be more vulnerable to PHMB treatment. The results described above show that PDI is an important gene for PHMB tolerance ability in a highly PHMB-tolerant strain of Acanthamoeba and provide a new insight for more efficient medicine development for Acanthamoeba keratitis. |
| first_indexed | 2024-12-10T13:35:27Z |
| format | Article |
| id | doaj.art-0766ca6e68f3493a8bc66a03ac52935f |
| institution | Directory Open Access Journal |
| issn | 2211-3207 |
| language | English |
| last_indexed | 2024-12-10T13:35:27Z |
| publishDate | 2016-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | International Journal for Parasitology: Drugs and Drug Resistance |
| spelling | doaj.art-0766ca6e68f3493a8bc66a03ac52935f2022-12-22T01:46:49ZengElsevierInternational Journal for Parasitology: Drugs and Drug Resistance2211-32072016-12-016325125710.1016/j.ijpddr.2016.11.001The effect of the disulfideisomerase domain containing protein in the defense against polyhexamethylene biguanide of highly tolerant Acanthamoeba at the trophozoite stageFu-Chin Huang0Tao-Shen Liu1Sung-Chou Li2Min-Hsiu Shih3Jyh-Wei Shin4Wei-Chen Lin5Department of Ophthalmology, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan, TaiwanDepartment of Microbiology and Immunology, College of Medicine, National Cheng Kung University, Tainan, TaiwanGenomics and Proteomics Core Laboratory, Department of Medical Research, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung, TaiwanDepartment of Ophthalmology, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan, TaiwanDepartment of Microbiology and Immunology, College of Medicine, National Cheng Kung University, Tainan, TaiwanDepartment of Microbiology and Immunology, College of Medicine, National Cheng Kung University, Tainan, TaiwanAcanthamoeba castellanii is a free-living protozoan pathogen capable of causing a blinding keratitis and fatal granulomatous encephalitis. Current treatment generally involves an hourly application of polyhexamethylene biguanide (PHMB) over a period of several days but this is not entirely effective against all strains/isolates. The tolerance mechanisms of PHMB in Acanthamoeba cells remain unclear. In this study, we found that the mRNA expression level of disulfideisomerase domain containing protein (PDI) increased rapidly in surviving cells of the highly PHMB-tolerant Acanthamoeba castellanii strain, NCKH_D, during PHMB treatment, but not in the ATCC standard strain. After PDI-specific silencing, NCKH_D was found to be more vulnerable to PHMB treatment. The results described above show that PDI is an important gene for PHMB tolerance ability in a highly PHMB-tolerant strain of Acanthamoeba and provide a new insight for more efficient medicine development for Acanthamoeba keratitis.http://www.sciencedirect.com/science/article/pii/S2211320716300768Acanthamoeba keratitisPolyhexamethylene biguanideDisulfideisomerase domain containing protein |
| spellingShingle | Fu-Chin Huang Tao-Shen Liu Sung-Chou Li Min-Hsiu Shih Jyh-Wei Shin Wei-Chen Lin The effect of the disulfideisomerase domain containing protein in the defense against polyhexamethylene biguanide of highly tolerant Acanthamoeba at the trophozoite stage International Journal for Parasitology: Drugs and Drug Resistance Acanthamoeba keratitis Polyhexamethylene biguanide Disulfideisomerase domain containing protein |
| title | The effect of the disulfideisomerase domain containing protein in the defense against polyhexamethylene biguanide of highly tolerant Acanthamoeba at the trophozoite stage |
| title_full | The effect of the disulfideisomerase domain containing protein in the defense against polyhexamethylene biguanide of highly tolerant Acanthamoeba at the trophozoite stage |
| title_fullStr | The effect of the disulfideisomerase domain containing protein in the defense against polyhexamethylene biguanide of highly tolerant Acanthamoeba at the trophozoite stage |
| title_full_unstemmed | The effect of the disulfideisomerase domain containing protein in the defense against polyhexamethylene biguanide of highly tolerant Acanthamoeba at the trophozoite stage |
| title_short | The effect of the disulfideisomerase domain containing protein in the defense against polyhexamethylene biguanide of highly tolerant Acanthamoeba at the trophozoite stage |
| title_sort | effect of the disulfideisomerase domain containing protein in the defense against polyhexamethylene biguanide of highly tolerant acanthamoeba at the trophozoite stage |
| topic | Acanthamoeba keratitis Polyhexamethylene biguanide Disulfideisomerase domain containing protein |
| url | http://www.sciencedirect.com/science/article/pii/S2211320716300768 |
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