CRISPR-Assisted Multiplex Base Editing System in Pseudomonas putida KT2440

CRISPR-Assisted Multiplex Base Editing System in Pseudomonas putida KT2440

Pseudomonas putida (P. putida) KT2440 is a paradigmatic environmental-bacterium that possesses significant potential in synthetic biology, metabolic engineering and biodegradation applications. However, most genome editing methods of P. putida KT2440 depend on heterologous repair proteins and the pr...

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Bibliographic Details
Main Authors:	Jun Sun, Li-Bing Lu, Tian-Xin Liang, Li-Rong Yang, Jian-Ping Wu
Format:	Article
Language:	English
Published:	Frontiers Media S.A. 2020-07-01
Series:	Frontiers in Bioengineering and Biotechnology
Subjects:	Pseudomonas putida KT2440 cytidine deaminase base editing gene inactivation multiplex genome editing Cas9 nickase
Online Access:	https://www.frontiersin.org/article/10.3389/fbioe.2020.00905/full

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