Natural Infections of Potato Plants Grown from Minitubers with Blackleg-Causing Soft Rot Pectobacteriaceae
Information on the infection incidence of blackleg-causing soft rot Pectobacteriaceae (BL-SRP) in potato crops grown from minitubers (PB1-crop) and the distribution of BL-SRP in individual plants was collected during a two-year survey conducted at five potato growers located in the Netherlands. In t...
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MDPI AG
2022-12-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/10/12/2504 |
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| author | Jan van der Wolf Marjon Krijger Odette Mendes Viola Kurm Jack Gros |
| author_facet | Jan van der Wolf Marjon Krijger Odette Mendes Viola Kurm Jack Gros |
| author_sort | Jan van der Wolf |
| collection | DOAJ |
| description | Information on the infection incidence of blackleg-causing soft rot Pectobacteriaceae (BL-SRP) in potato crops grown from minitubers (PB1-crop) and the distribution of BL-SRP in individual plants was collected during a two-year survey conducted at five potato growers located in the Netherlands. In the last weeks before haulm destruction, leaves, stems, and tubers of 100 or 200 plants were analyzed separately for the presence of <i>Pectobacterium parmentieri</i>, <i>P. brasiliense</i>, <i>P. atrosepticum</i>, and <i>Dickeya</i> spp. Extracted plant parts enriched for BL-SRP were analyzed with TaqMan assays specific for the detection of blackleg-causing BL-SRP. In 2019, low incidences of <i>P. parmentieri</i> (1–6%) in leaves were found at four growing sites. At one farm, reactions were detected in TaqMan assays for <i>D. zeae</i> and <i>D. chrysanthemi</i> in leaves. In 2020, the crops of two growers were largely free from BL-SRP. At one farm, a high infection incidence (21%) was found for <i>D. fangzhongdai</i> in tubers. The isolated pathogen was able to cause potato blackleg. At two other farms, high infection incidences in tubers were found with <i>P. brasiliense</i> (35–39%) and <i>P. parmentieri</i> (12–19%), whereas the incidence of <i>P. brasiliense</i> in leaves was also high (8%). In conclusion, high infection incidences with BL-SRP in potatoes can be found in a PB1 crop at the end of the growing season. Infections in individual plants were found either in tubers or in leaves. The potential sources of initial infection are discussed. |
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| language | English |
| last_indexed | 2024-03-09T16:03:49Z |
| publishDate | 2022-12-01 |
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| series | Microorganisms |
| spelling | doaj.art-076b7c59c5b04c5098aec01cb52e9d152023-11-24T16:50:13ZengMDPI AGMicroorganisms2076-26072022-12-011012250410.3390/microorganisms10122504Natural Infections of Potato Plants Grown from Minitubers with Blackleg-Causing Soft Rot PectobacteriaceaeJan van der Wolf0Marjon Krijger1Odette Mendes2Viola Kurm3Jack Gros4Biointeractions and Plant Health, Wageningen University & Research, Droevendaalsesteeg 1, 6708 PB Wageningen, The NetherlandsBiointeractions and Plant Health, Wageningen University & Research, Droevendaalsesteeg 1, 6708 PB Wageningen, The NetherlandsBiointeractions and Plant Health, Wageningen University & Research, Droevendaalsesteeg 1, 6708 PB Wageningen, The NetherlandsBiointeractions and Plant Health, Wageningen University & Research, Droevendaalsesteeg 1, 6708 PB Wageningen, The NetherlandsAgrico Research, Burchtweg 17, 8314 PP Bant, The NetherlandsInformation on the infection incidence of blackleg-causing soft rot Pectobacteriaceae (BL-SRP) in potato crops grown from minitubers (PB1-crop) and the distribution of BL-SRP in individual plants was collected during a two-year survey conducted at five potato growers located in the Netherlands. In the last weeks before haulm destruction, leaves, stems, and tubers of 100 or 200 plants were analyzed separately for the presence of <i>Pectobacterium parmentieri</i>, <i>P. brasiliense</i>, <i>P. atrosepticum</i>, and <i>Dickeya</i> spp. Extracted plant parts enriched for BL-SRP were analyzed with TaqMan assays specific for the detection of blackleg-causing BL-SRP. In 2019, low incidences of <i>P. parmentieri</i> (1–6%) in leaves were found at four growing sites. At one farm, reactions were detected in TaqMan assays for <i>D. zeae</i> and <i>D. chrysanthemi</i> in leaves. In 2020, the crops of two growers were largely free from BL-SRP. At one farm, a high infection incidence (21%) was found for <i>D. fangzhongdai</i> in tubers. The isolated pathogen was able to cause potato blackleg. At two other farms, high infection incidences in tubers were found with <i>P. brasiliense</i> (35–39%) and <i>P. parmentieri</i> (12–19%), whereas the incidence of <i>P. brasiliense</i> in leaves was also high (8%). In conclusion, high infection incidences with BL-SRP in potatoes can be found in a PB1 crop at the end of the growing season. Infections in individual plants were found either in tubers or in leaves. The potential sources of initial infection are discussed.https://www.mdpi.com/2076-2607/10/12/2504<i>Pectobacterium</i><i>Dickeya</i>TaqManinfection sourceairborne infectionsoilborne infection |
| spellingShingle | Jan van der Wolf Marjon Krijger Odette Mendes Viola Kurm Jack Gros Natural Infections of Potato Plants Grown from Minitubers with Blackleg-Causing Soft Rot Pectobacteriaceae Microorganisms <i>Pectobacterium</i> <i>Dickeya</i> TaqMan infection source airborne infection soilborne infection |
| title | Natural Infections of Potato Plants Grown from Minitubers with Blackleg-Causing Soft Rot Pectobacteriaceae |
| title_full | Natural Infections of Potato Plants Grown from Minitubers with Blackleg-Causing Soft Rot Pectobacteriaceae |
| title_fullStr | Natural Infections of Potato Plants Grown from Minitubers with Blackleg-Causing Soft Rot Pectobacteriaceae |
| title_full_unstemmed | Natural Infections of Potato Plants Grown from Minitubers with Blackleg-Causing Soft Rot Pectobacteriaceae |
| title_short | Natural Infections of Potato Plants Grown from Minitubers with Blackleg-Causing Soft Rot Pectobacteriaceae |
| title_sort | natural infections of potato plants grown from minitubers with blackleg causing soft rot pectobacteriaceae |
| topic | <i>Pectobacterium</i> <i>Dickeya</i> TaqMan infection source airborne infection soilborne infection |
| url | https://www.mdpi.com/2076-2607/10/12/2504 |
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