Genome-Wide Association Analysis of Cowpea Mild Mottle Virus Resistance in Soybean Germplasms from Northeast China
Cowpea mild mottle virus (CpMMV) is an important viral pathogen that seriously influences the yield and seed quality of soybeans worldwide. Resistance breeding is one of the most effective, economical, and environmentally safe strategies for controlling the disease caused by CpMMV. However, only few...
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MDPI AG
2024-02-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/14/3/489 |
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| author | Yameng Luan Siqi Yang Yuting Wang Yu Zhao Xiaoyun Wu Qingshan Chen Zhaoming Qi Xiaoxia Wu Weiqin Ji Xiaofei Cheng |
| author_facet | Yameng Luan Siqi Yang Yuting Wang Yu Zhao Xiaoyun Wu Qingshan Chen Zhaoming Qi Xiaoxia Wu Weiqin Ji Xiaofei Cheng |
| author_sort | Yameng Luan |
| collection | DOAJ |
| description | Cowpea mild mottle virus (CpMMV) is an important viral pathogen that seriously influences the yield and seed quality of soybeans worldwide. Resistance breeding is one of the most effective, economical, and environmentally safe strategies for controlling the disease caused by CpMMV. However, only few resistance genes have been identified in soybeans. In this study, the resistance of 169 soybean germplasms from Northeast China to a CpMMV strain isolated from soybean in China was evaluated, and a genome-wide association study (GWAS) was then performed to find possible resistance genes in these soybean germplasms. Nine resistant soybean germplasms were identified and two single nucleotide polymorphism sites (SNPs) were found to be closely associated with CpMMV resistance. A total number of 51 and 25 candidate genes neighboring the resistance-associated SNPs on chromosomes 6 and 12, respectively, were identified, among which one receptor-like kinase (RLK) on chromosome 6 and 2 toll-interleukin-1 receptor nucleotide-binding leucine-rich repeat receptors (TNLs) on chromosome 12 were recognized as the most probable resistance genes, respectively. Together, these data provide new insights on the resistance resources of soybeans to CpMMV, which will benefit the breeding of CpMMV-resistant soybean cultivars. |
| first_indexed | 2024-04-24T18:38:25Z |
| format | Article |
| id | doaj.art-0775eb78fa264d67ad679646c48dd3b7 |
| institution | Directory Open Access Journal |
| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-04-24T18:38:25Z |
| publishDate | 2024-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj.art-0775eb78fa264d67ad679646c48dd3b72024-03-27T13:16:41ZengMDPI AGAgronomy2073-43952024-02-0114348910.3390/agronomy14030489Genome-Wide Association Analysis of Cowpea Mild Mottle Virus Resistance in Soybean Germplasms from Northeast ChinaYameng Luan0Siqi Yang1Yuting Wang2Yu Zhao3Xiaoyun Wu4Qingshan Chen5Zhaoming Qi6Xiaoxia Wu7Weiqin Ji8Xiaofei Cheng9College of Plant Protection, Northeast Agricultural University, Harbin 150030, ChinaCollege of Agriculture, Northeast Agricultural University, Harbin 150030, ChinaCollege of Plant Protection, Northeast Agricultural University, Harbin 150030, ChinaCollege of Plant Protection, Northeast Agricultural University, Harbin 150030, ChinaCollege of Plant Protection, Northeast Agricultural University, Harbin 150030, ChinaCollege of Agriculture, Northeast Agricultural University, Harbin 150030, ChinaCollege of Agriculture, Northeast Agricultural University, Harbin 150030, ChinaCollege of Life Science, Northeast Agricultural University, Harbin 150030, ChinaCollege of Plant Protection, Northeast Agricultural University, Harbin 150030, ChinaCollege of Plant Protection, Northeast Agricultural University, Harbin 150030, ChinaCowpea mild mottle virus (CpMMV) is an important viral pathogen that seriously influences the yield and seed quality of soybeans worldwide. Resistance breeding is one of the most effective, economical, and environmentally safe strategies for controlling the disease caused by CpMMV. However, only few resistance genes have been identified in soybeans. In this study, the resistance of 169 soybean germplasms from Northeast China to a CpMMV strain isolated from soybean in China was evaluated, and a genome-wide association study (GWAS) was then performed to find possible resistance genes in these soybean germplasms. Nine resistant soybean germplasms were identified and two single nucleotide polymorphism sites (SNPs) were found to be closely associated with CpMMV resistance. A total number of 51 and 25 candidate genes neighboring the resistance-associated SNPs on chromosomes 6 and 12, respectively, were identified, among which one receptor-like kinase (RLK) on chromosome 6 and 2 toll-interleukin-1 receptor nucleotide-binding leucine-rich repeat receptors (TNLs) on chromosome 12 were recognized as the most probable resistance genes, respectively. Together, these data provide new insights on the resistance resources of soybeans to CpMMV, which will benefit the breeding of CpMMV-resistant soybean cultivars.https://www.mdpi.com/2073-4395/14/3/489soybeanCpMMVGWASSNPresistance genes |
| spellingShingle | Yameng Luan Siqi Yang Yuting Wang Yu Zhao Xiaoyun Wu Qingshan Chen Zhaoming Qi Xiaoxia Wu Weiqin Ji Xiaofei Cheng Genome-Wide Association Analysis of Cowpea Mild Mottle Virus Resistance in Soybean Germplasms from Northeast China Agronomy soybean CpMMV GWAS SNP resistance genes |
| title | Genome-Wide Association Analysis of Cowpea Mild Mottle Virus Resistance in Soybean Germplasms from Northeast China |
| title_full | Genome-Wide Association Analysis of Cowpea Mild Mottle Virus Resistance in Soybean Germplasms from Northeast China |
| title_fullStr | Genome-Wide Association Analysis of Cowpea Mild Mottle Virus Resistance in Soybean Germplasms from Northeast China |
| title_full_unstemmed | Genome-Wide Association Analysis of Cowpea Mild Mottle Virus Resistance in Soybean Germplasms from Northeast China |
| title_short | Genome-Wide Association Analysis of Cowpea Mild Mottle Virus Resistance in Soybean Germplasms from Northeast China |
| title_sort | genome wide association analysis of cowpea mild mottle virus resistance in soybean germplasms from northeast china |
| topic | soybean CpMMV GWAS SNP resistance genes |
| url | https://www.mdpi.com/2073-4395/14/3/489 |
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