Procedures to characterize and study P2Z/P2X7 purinoceptor: flow cytometry as a promising practical, reliable tool
The expression of P2Z/P2X7 purinoceptor in different cell types is well established. This receptor is a member of the ionotropic P2X receptor family, which is composed by seven cloned receptor subtypes (P2X1 - P2X7). Interestingly, the P2Z/P2X7 has a unique feature of being linked to a non-selective...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Fundação Oswaldo Cruz (FIOCRUZ)
2000-01-01
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| Series: | Memorias do Instituto Oswaldo Cruz |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762000000300019 |
| _version_ | 1797757658856423424 |
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| author | Nihei Oscar Kenji Savino Wilson Alves Luiz Anastacio |
| author_facet | Nihei Oscar Kenji Savino Wilson Alves Luiz Anastacio |
| author_sort | Nihei Oscar Kenji |
| collection | DOAJ |
| description | The expression of P2Z/P2X7 purinoceptor in different cell types is well established. This receptor is a member of the ionotropic P2X receptor family, which is composed by seven cloned receptor subtypes (P2X1 - P2X7). Interestingly, the P2Z/P2X7 has a unique feature of being linked to a non-selective pore which allows the passage of molecules up to 900 Da depending on the cell type. Early studies of P2Z/P2X7 purinoceptor were exclusively based on classical pharmacological studies but the recent tools of molecular biology have enriched the analysis of the receptor expression. The majority of assays and techniques chosen so far to study the expression of P2Z/P2X7 receptor explore directly or indirectly the effects of the opening of P2Z/P2X7 linked pore. In this review we describe the main techniques used to study the expression and functionality of P2Z/P2X7 receptor. Additionally, the increasing need and importance of a multifunctional analysis of P2Z/P2X7 expression based on flow cytometry technology is discussed, as well as the adoption of a more complete analysis of P2Z/P2X7 expression involving different techniques. |
| first_indexed | 2024-03-12T18:18:50Z |
| format | Article |
| id | doaj.art-07762fdd71194d33ae610cb67df3a9b3 |
| institution | Directory Open Access Journal |
| issn | 0074-0276 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T18:18:50Z |
| publishDate | 2000-01-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-07762fdd71194d33ae610cb67df3a9b32023-08-02T08:59:59ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz0074-02761678-80602000-01-01953415428Procedures to characterize and study P2Z/P2X7 purinoceptor: flow cytometry as a promising practical, reliable toolNihei Oscar KenjiSavino WilsonAlves Luiz AnastacioThe expression of P2Z/P2X7 purinoceptor in different cell types is well established. This receptor is a member of the ionotropic P2X receptor family, which is composed by seven cloned receptor subtypes (P2X1 - P2X7). Interestingly, the P2Z/P2X7 has a unique feature of being linked to a non-selective pore which allows the passage of molecules up to 900 Da depending on the cell type. Early studies of P2Z/P2X7 purinoceptor were exclusively based on classical pharmacological studies but the recent tools of molecular biology have enriched the analysis of the receptor expression. The majority of assays and techniques chosen so far to study the expression of P2Z/P2X7 receptor explore directly or indirectly the effects of the opening of P2Z/P2X7 linked pore. In this review we describe the main techniques used to study the expression and functionality of P2Z/P2X7 receptor. Additionally, the increasing need and importance of a multifunctional analysis of P2Z/P2X7 expression based on flow cytometry technology is discussed, as well as the adoption of a more complete analysis of P2Z/P2X7 expression involving different techniques.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762000000300019flow cytometrypurinergic receptorsP2X7 receptorethidium bromideextracellular ATP |
| spellingShingle | Nihei Oscar Kenji Savino Wilson Alves Luiz Anastacio Procedures to characterize and study P2Z/P2X7 purinoceptor: flow cytometry as a promising practical, reliable tool Memorias do Instituto Oswaldo Cruz flow cytometry purinergic receptors P2X7 receptor ethidium bromide extracellular ATP |
| title | Procedures to characterize and study P2Z/P2X7 purinoceptor: flow cytometry as a promising practical, reliable tool |
| title_full | Procedures to characterize and study P2Z/P2X7 purinoceptor: flow cytometry as a promising practical, reliable tool |
| title_fullStr | Procedures to characterize and study P2Z/P2X7 purinoceptor: flow cytometry as a promising practical, reliable tool |
| title_full_unstemmed | Procedures to characterize and study P2Z/P2X7 purinoceptor: flow cytometry as a promising practical, reliable tool |
| title_short | Procedures to characterize and study P2Z/P2X7 purinoceptor: flow cytometry as a promising practical, reliable tool |
| title_sort | procedures to characterize and study p2z p2x7 purinoceptor flow cytometry as a promising practical reliable tool |
| topic | flow cytometry purinergic receptors P2X7 receptor ethidium bromide extracellular ATP |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762000000300019 |
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