Acadesine supplementation in a soybean oil-based diet remodels hepatic lipid and glucose metabolism in juvenile large yellow croaker (Larimichthys crocea)
Dietary high soybean oil (SO) inclusion leads to excessive hepatic lipid accumulation and metabolism disorder in fish species. This study was conducted to investigate the effects of adenosine 5’-monophosphate-activated protein kinase (AMPK) pathway activation by acadesine (AICAR) on hepatic histolog...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-06-01
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| Series: | Aquaculture Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352513423000881 |
| _version_ | 1797801747731709952 |
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| author | Peng Tan Chaoqing Wei Shanshan Zhu Yu Zhang Wanjie Liu Ruiyi Chen Ligai Wang Hua Mu Dongdong Xu |
| author_facet | Peng Tan Chaoqing Wei Shanshan Zhu Yu Zhang Wanjie Liu Ruiyi Chen Ligai Wang Hua Mu Dongdong Xu |
| author_sort | Peng Tan |
| collection | DOAJ |
| description | Dietary high soybean oil (SO) inclusion leads to excessive hepatic lipid accumulation and metabolism disorder in fish species. This study was conducted to investigate the effects of adenosine 5’-monophosphate-activated protein kinase (AMPK) pathway activation by acadesine (AICAR) on hepatic histology and metabolism-related gene expression of juvenile large yellow croakers (Larimichthys crocea) fed with SO-based diet. In this study, fish (initial body weight 3.89 ± 0.02 g) were fed fish oil (FO)-based, SO, or SOA (SO diet supplemented with 100 mg kg−1 AICAR) diets with three replicates of each treatment for 70 days. In general, the replacement of dietary FO with SO led to excessive hepatic lipid accumulation and a glucose metabolism disorder. Histological sections and biochemical analysis of hepatic tissue consistently revealed that AICAR supplementation could partly ameliorate SO-induced excessive hepatic lipid accumulation, probably by suppressing the expression of genes related to fatty acid uptake and lipogenesis while enhancing the expression of lipolysis-related genes. Nevertheless, AICAR regulated lipid metabolism in muscle tissue in an inverse pattern to that in hepatic tissue, indicating that muscle tissue could attempt to mitigate lipid accumulation by improving lipolysis in response to the dietary inclusion of SO. Dietary AICAR partly improved glucose metabolism by reducing the expression of genes related to gluconeogenesis in hepatic tissue while increasing the expression of genes related to glucose uptake and glycolysis in muscle tissue. Collectively, dietary AICAR mitigated excessive hepatic lipid accumulation and glucose metabolism disorder. The amelioration of hepatic lipid accumulation by AICAR is likely caused by increasing lipid catabolism and decreasing lipid anabolism. The improved glucose metabolism by AICAR may be ascribed to decreased hepatic gluconeogenesis, as well as increased muscle glucose uptake and utilization. |
| first_indexed | 2024-03-13T04:55:11Z |
| format | Article |
| id | doaj.art-077b5e00c07346ff8dbc0cdb09a8bf95 |
| institution | Directory Open Access Journal |
| issn | 2352-5134 |
| language | English |
| last_indexed | 2024-03-13T04:55:11Z |
| publishDate | 2023-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Aquaculture Reports |
| spelling | doaj.art-077b5e00c07346ff8dbc0cdb09a8bf952023-06-18T05:02:19ZengElsevierAquaculture Reports2352-51342023-06-0130101549Acadesine supplementation in a soybean oil-based diet remodels hepatic lipid and glucose metabolism in juvenile large yellow croaker (Larimichthys crocea)Peng Tan0Chaoqing Wei1Shanshan Zhu2Yu Zhang3Wanjie Liu4Ruiyi Chen5Ligai Wang6Hua Mu7Dongdong Xu8Key Laboratory of Mariculture and Enhancement, Zhejiang Marine Fishery Research Institute, Zhoushan 316021, People’s Republic of ChinaJiangsu Key Laboratory of Marine Biotechnology, Jiangsu Key Laboratory of Marine Bioresources and Environment, Jiangsu Ocean University, Lianyungang 222005, People’s Republic of China; Co-Innovation Center of Jiangsu Marine Bio-Industry Technology, Jiangsu Ocean University, Lianyungang 222005, People’s Republic of China; Marine Resource Development Institute of Jiangsu (Lianyungang), Lianyungang 222005, People’s Republic of ChinaJiangsu Key Laboratory of Marine Biotechnology, Jiangsu Key Laboratory of Marine Bioresources and Environment, Jiangsu Ocean University, Lianyungang 222005, People’s Republic of ChinaJiangsu Key Laboratory of Marine Biotechnology, Jiangsu Key Laboratory of Marine Bioresources and Environment, Jiangsu Ocean University, Lianyungang 222005, People’s Republic of ChinaJiangsu Key Laboratory of Marine Biotechnology, Jiangsu Key Laboratory of Marine Bioresources and Environment, Jiangsu Ocean University, Lianyungang 222005, People’s Republic of ChinaKey Laboratory of Mariculture and Enhancement, Zhejiang Marine Fishery Research Institute, Zhoushan 316021, People’s Republic of ChinaKey Laboratory of Mariculture and Enhancement, Zhejiang Marine Fishery Research Institute, Zhoushan 316021, People’s Republic of ChinaJiangsu Key Laboratory of Marine Biotechnology, Jiangsu Key Laboratory of Marine Bioresources and Environment, Jiangsu Ocean University, Lianyungang 222005, People’s Republic of China; Co-Innovation Center of Jiangsu Marine Bio-Industry Technology, Jiangsu Ocean University, Lianyungang 222005, People’s Republic of China; Marine Resource Development Institute of Jiangsu (Lianyungang), Lianyungang 222005, People’s Republic of China; Corresponding author at: Jiangsu Key Laboratory of Marine Biotechnology, Jiangsu Key Laboratory of Marine Bioresources and Environment, Jiangsu Ocean University, Lianyungang 222005, People’s Republic of China.Key Laboratory of Mariculture and Enhancement, Zhejiang Marine Fishery Research Institute, Zhoushan 316021, People’s Republic of China; Corresponding author.Dietary high soybean oil (SO) inclusion leads to excessive hepatic lipid accumulation and metabolism disorder in fish species. This study was conducted to investigate the effects of adenosine 5’-monophosphate-activated protein kinase (AMPK) pathway activation by acadesine (AICAR) on hepatic histology and metabolism-related gene expression of juvenile large yellow croakers (Larimichthys crocea) fed with SO-based diet. In this study, fish (initial body weight 3.89 ± 0.02 g) were fed fish oil (FO)-based, SO, or SOA (SO diet supplemented with 100 mg kg−1 AICAR) diets with three replicates of each treatment for 70 days. In general, the replacement of dietary FO with SO led to excessive hepatic lipid accumulation and a glucose metabolism disorder. Histological sections and biochemical analysis of hepatic tissue consistently revealed that AICAR supplementation could partly ameliorate SO-induced excessive hepatic lipid accumulation, probably by suppressing the expression of genes related to fatty acid uptake and lipogenesis while enhancing the expression of lipolysis-related genes. Nevertheless, AICAR regulated lipid metabolism in muscle tissue in an inverse pattern to that in hepatic tissue, indicating that muscle tissue could attempt to mitigate lipid accumulation by improving lipolysis in response to the dietary inclusion of SO. Dietary AICAR partly improved glucose metabolism by reducing the expression of genes related to gluconeogenesis in hepatic tissue while increasing the expression of genes related to glucose uptake and glycolysis in muscle tissue. Collectively, dietary AICAR mitigated excessive hepatic lipid accumulation and glucose metabolism disorder. The amelioration of hepatic lipid accumulation by AICAR is likely caused by increasing lipid catabolism and decreasing lipid anabolism. The improved glucose metabolism by AICAR may be ascribed to decreased hepatic gluconeogenesis, as well as increased muscle glucose uptake and utilization.http://www.sciencedirect.com/science/article/pii/S2352513423000881AcadesineSoybean oilLipid metabolismGlucose metabolismLarge yellow croaker |
| spellingShingle | Peng Tan Chaoqing Wei Shanshan Zhu Yu Zhang Wanjie Liu Ruiyi Chen Ligai Wang Hua Mu Dongdong Xu Acadesine supplementation in a soybean oil-based diet remodels hepatic lipid and glucose metabolism in juvenile large yellow croaker (Larimichthys crocea) Aquaculture Reports Acadesine Soybean oil Lipid metabolism Glucose metabolism Large yellow croaker |
| title | Acadesine supplementation in a soybean oil-based diet remodels hepatic lipid and glucose metabolism in juvenile large yellow croaker (Larimichthys crocea) |
| title_full | Acadesine supplementation in a soybean oil-based diet remodels hepatic lipid and glucose metabolism in juvenile large yellow croaker (Larimichthys crocea) |
| title_fullStr | Acadesine supplementation in a soybean oil-based diet remodels hepatic lipid and glucose metabolism in juvenile large yellow croaker (Larimichthys crocea) |
| title_full_unstemmed | Acadesine supplementation in a soybean oil-based diet remodels hepatic lipid and glucose metabolism in juvenile large yellow croaker (Larimichthys crocea) |
| title_short | Acadesine supplementation in a soybean oil-based diet remodels hepatic lipid and glucose metabolism in juvenile large yellow croaker (Larimichthys crocea) |
| title_sort | acadesine supplementation in a soybean oil based diet remodels hepatic lipid and glucose metabolism in juvenile large yellow croaker larimichthys crocea |
| topic | Acadesine Soybean oil Lipid metabolism Glucose metabolism Large yellow croaker |
| url | http://www.sciencedirect.com/science/article/pii/S2352513423000881 |
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