Comparison of the efficacy of human umbilical cord mesenchymal stem cells conditioned medium and platelet-rich plasma on the hippocampus of STZ–induced rat model of Alzheimer’s disease: A behavioral and stereological study
Introduction: Alzheimer’s disease (AD) is accompanied by progressive cognitive disorders and memory loss. This study aims to determine the combined effects of conditioned medium of human umbilical cord mesenchymal stem cells (CM) and platelet-rich plasma (PRP) on AD model rats. Methods: Forty-eight...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-12-01
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| Series: | IBRO Neuroscience Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2667242123022637 |
| _version_ | 1797383632129622016 |
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| author | Amin Firoozi Aliakbar Alizadeh Asadollah Zarifkar Tahereh Esmaeilpour Mohammad Reza Namavar Omid Alavi Farzaneh Dehghani |
| author_facet | Amin Firoozi Aliakbar Alizadeh Asadollah Zarifkar Tahereh Esmaeilpour Mohammad Reza Namavar Omid Alavi Farzaneh Dehghani |
| author_sort | Amin Firoozi |
| collection | DOAJ |
| description | Introduction: Alzheimer’s disease (AD) is accompanied by progressive cognitive disorders and memory loss. This study aims to determine the combined effects of conditioned medium of human umbilical cord mesenchymal stem cells (CM) and platelet-rich plasma (PRP) on AD model rats. Methods: Forty-eight male Sprague Dawley rats were classified into 6 groups: Control, Sham, AD, and three treatment groups. AD was induced by streptozotocin(STZ; 3 mg/kg, intracerebroventricular (ICV)) and the treatment groups received injections of CM [(200 µl, intraperitoneally (i.p.), and/or PRP (100 µl, intravenously(i.v)] for 8 days. Behavioral tests (Morris water maze and novel objective recognition) were used to assess learning ability and memory. At the end of the behavioral tests, the rats were sacrificed and their brain was entirely removed, sectioned, and stained with cresyl violet. The hippocampus volume and number of neurons were evaluated by stereological techniques. Results: In the AD group, the discrimination ratio, time spent in the target zone, volume of Cornu Ammonis1 (CA1) and Dentate Gyrus (DG), and the number of pyramidal and granular cells decreased significantly compared to the Sham group. The mentioned parameters increased in the CM and CM+PRP groups compared to the AD group (p < 0.01). PRP did not have any noticeable effect on the examined parameters. Conclusions: CM may be beneficial in the treatment of AD as it led to better improvement in STZ-induced learning and memory impairments as well as the structure of the hippocampus. |
| first_indexed | 2024-03-08T21:23:52Z |
| format | Article |
| id | doaj.art-077ec8b0ddcf48a0bbe2211002adb85c |
| institution | Directory Open Access Journal |
| issn | 2667-2421 |
| language | English |
| last_indexed | 2024-03-08T21:23:52Z |
| publishDate | 2023-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | IBRO Neuroscience Reports |
| spelling | doaj.art-077ec8b0ddcf48a0bbe2211002adb85c2023-12-21T07:38:33ZengElsevierIBRO Neuroscience Reports2667-24212023-12-0115209217Comparison of the efficacy of human umbilical cord mesenchymal stem cells conditioned medium and platelet-rich plasma on the hippocampus of STZ–induced rat model of Alzheimer’s disease: A behavioral and stereological studyAmin Firoozi0Aliakbar Alizadeh1Asadollah Zarifkar2Tahereh Esmaeilpour3Mohammad Reza Namavar4Omid Alavi5Farzaneh Dehghani6Department of Anatomy, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran; Histomorphometry & Stereology Research Center, Shiraz University of Medical Sciences, Shiraz, IranDepartment of Tissue Engineering and Applied Cell Sciences, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, IranNeuroscience Research Center and Department of Physiology, Shiraz University of Medical Sciences, Shiraz, IranDepartment of Anatomy, School of Medicine, Shiraz University of Medical Sciences, Shiraz, IranDepartment of Anatomy, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran; Histomorphometry & Stereology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran; Clinical Neurology Research Center, Shiraz University of Medical Sciences, Shiraz, IranDepartment of Tissue Engineering and Applied Cell Sciences, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, IranDepartment of Anatomy, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran; Histomorphometry & Stereology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran; Corresponding author at: Department of Anatomy, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.Introduction: Alzheimer’s disease (AD) is accompanied by progressive cognitive disorders and memory loss. This study aims to determine the combined effects of conditioned medium of human umbilical cord mesenchymal stem cells (CM) and platelet-rich plasma (PRP) on AD model rats. Methods: Forty-eight male Sprague Dawley rats were classified into 6 groups: Control, Sham, AD, and three treatment groups. AD was induced by streptozotocin(STZ; 3 mg/kg, intracerebroventricular (ICV)) and the treatment groups received injections of CM [(200 µl, intraperitoneally (i.p.), and/or PRP (100 µl, intravenously(i.v)] for 8 days. Behavioral tests (Morris water maze and novel objective recognition) were used to assess learning ability and memory. At the end of the behavioral tests, the rats were sacrificed and their brain was entirely removed, sectioned, and stained with cresyl violet. The hippocampus volume and number of neurons were evaluated by stereological techniques. Results: In the AD group, the discrimination ratio, time spent in the target zone, volume of Cornu Ammonis1 (CA1) and Dentate Gyrus (DG), and the number of pyramidal and granular cells decreased significantly compared to the Sham group. The mentioned parameters increased in the CM and CM+PRP groups compared to the AD group (p < 0.01). PRP did not have any noticeable effect on the examined parameters. Conclusions: CM may be beneficial in the treatment of AD as it led to better improvement in STZ-induced learning and memory impairments as well as the structure of the hippocampus.http://www.sciencedirect.com/science/article/pii/S2667242123022637Alzheimer’s diseasePlatelet-rich plasmaHuman umbilical cord mesenchymal stem cellsStereologyHippocampus |
| spellingShingle | Amin Firoozi Aliakbar Alizadeh Asadollah Zarifkar Tahereh Esmaeilpour Mohammad Reza Namavar Omid Alavi Farzaneh Dehghani Comparison of the efficacy of human umbilical cord mesenchymal stem cells conditioned medium and platelet-rich plasma on the hippocampus of STZ–induced rat model of Alzheimer’s disease: A behavioral and stereological study IBRO Neuroscience Reports Alzheimer’s disease Platelet-rich plasma Human umbilical cord mesenchymal stem cells Stereology Hippocampus |
| title | Comparison of the efficacy of human umbilical cord mesenchymal stem cells conditioned medium and platelet-rich plasma on the hippocampus of STZ–induced rat model of Alzheimer’s disease: A behavioral and stereological study |
| title_full | Comparison of the efficacy of human umbilical cord mesenchymal stem cells conditioned medium and platelet-rich plasma on the hippocampus of STZ–induced rat model of Alzheimer’s disease: A behavioral and stereological study |
| title_fullStr | Comparison of the efficacy of human umbilical cord mesenchymal stem cells conditioned medium and platelet-rich plasma on the hippocampus of STZ–induced rat model of Alzheimer’s disease: A behavioral and stereological study |
| title_full_unstemmed | Comparison of the efficacy of human umbilical cord mesenchymal stem cells conditioned medium and platelet-rich plasma on the hippocampus of STZ–induced rat model of Alzheimer’s disease: A behavioral and stereological study |
| title_short | Comparison of the efficacy of human umbilical cord mesenchymal stem cells conditioned medium and platelet-rich plasma on the hippocampus of STZ–induced rat model of Alzheimer’s disease: A behavioral and stereological study |
| title_sort | comparison of the efficacy of human umbilical cord mesenchymal stem cells conditioned medium and platelet rich plasma on the hippocampus of stz induced rat model of alzheimer s disease a behavioral and stereological study |
| topic | Alzheimer’s disease Platelet-rich plasma Human umbilical cord mesenchymal stem cells Stereology Hippocampus |
| url | http://www.sciencedirect.com/science/article/pii/S2667242123022637 |
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