An accurate, reliable, and universal qPCR method to identify homozygous single insert T-DNA with the example of transgenic rice

An accurate, reliable, and universal qPCR method to identify homozygous single insert T-DNA with the example of transgenic rice

Early determination of transgenic plants that are homozygous for a single locus T-DNA insert is highly desirable in most fundamental and applied transgenic research. This study aimed to build on an accurate, rapid, and reliable quantitative real-time PCR (qPCR) method to fast-track the development o...

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Bibliographic Details
Main Authors: Hai Thanh Tran, Carly Schramm, My-my Huynh, Yuri Shavrukov, James C. R. Stangoulis, Colin L. D. Jenkins, Peter A. Anderson
Format: Article
Language:English
Published: Frontiers Media S.A. 2023-10-01
Series:Frontiers in Plant Science
Subjects:
quantitative real-time PCR
zygosity identification
homozygous plant
NOS terminator
OsSBE4 reference gene
single T-DNA
Online Access:https://www.frontiersin.org/articles/10.3389/fpls.2023.1221790/full

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