An accurate, reliable, and universal qPCR method to identify homozygous single insert T-DNA with the example of transgenic rice
Early determination of transgenic plants that are homozygous for a single locus T-DNA insert is highly desirable in most fundamental and applied transgenic research. This study aimed to build on an accurate, rapid, and reliable quantitative real-time PCR (qPCR) method to fast-track the development o...
Main Authors: | Hai Thanh Tran, Carly Schramm, My-my Huynh, Yuri Shavrukov, James C. R. Stangoulis, Colin L. D. Jenkins, Peter A. Anderson |
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Format: | Article |
Language: | English |
Published: |
Frontiers Media S.A.
2023-10-01
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Series: | Frontiers in Plant Science |
Subjects: | |
Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2023.1221790/full |
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