The effects of Lactobacillus reuteri on the inflammation and periodontal tissue repair in rats: A pilot study
Objective: The aim of this study was to evaluate the effects of probiotic (PRO) Lactobacillus reuteri (DSM17938) as an adjuvant to the treatment of experimental periodontitis (EP). Material and methods: Fifty-four male adult Wistar rats were included. EP was induced and maintained for 7 days. Subseq...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-09-01
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| Series: | Saudi Dental Journal |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1013905222000608 |
| _version_ | 1811297289585557504 |
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| author | Valdir Gouveia Garcia Daniela Maria Janjácomo Miessi Tiago Esgalha da Rocha Natália Amanda Gomes Marta Aparecida Alberton Nuernberg Jânderson de Medeiros Cardoso Edilson Ervolino Letícia Helena Theodoro |
| author_facet | Valdir Gouveia Garcia Daniela Maria Janjácomo Miessi Tiago Esgalha da Rocha Natália Amanda Gomes Marta Aparecida Alberton Nuernberg Jânderson de Medeiros Cardoso Edilson Ervolino Letícia Helena Theodoro |
| author_sort | Valdir Gouveia Garcia |
| collection | DOAJ |
| description | Objective: The aim of this study was to evaluate the effects of probiotic (PRO) Lactobacillus reuteri (DSM17938) as an adjuvant to the treatment of experimental periodontitis (EP). Material and methods: Fifty-four male adult Wistar rats were included. EP was induced and maintained for 7 days. Subsequently, the ligature was removed and the animals were allocated into three different experimental groups (n = 18/group): EP – no local treatment, the animals received four systemic saline solution (SS) administrations; SRP+SS, the animals underwent SRP treatment, followed by SS administration; and SRP+PRO, the animals received SRP treatment, followed by the systemic administration of PROs (Lactobacillus reuteri ; 0.16 ml/day). Six animals from each group were euthanised at 7, 15 and 30 days. Histological and histometric analyses of alveolar bone loss (BL) and immunohistochemical analyses for TRAP, RANKL, OPG, OCN, and PCNA were performed. Shapiro–Wilk, ANOVA, post-hoc Tukey, Kruskal–Wallis, Student–Newman Keuls were performed. Results: The SRP+PRO group presented a reduction in inflammation. At 15 days, a lower BL was observed in the SRP+SS and SRP+PRO groups. Greater immunolabeling was noticed for PCNA at 15 days in the SRP+PRO group than in the SRP+SS group. The SRP+PRO group demonstrated a higher OCN immunolabeling pattern than the EP group at 15 and 30 days. Conclusion: The use of Lactobacillus reuteri as an adjuvant to SRP for the treatment of EP showed promising results in the control of local inflammatory responses, and enhanced the periodontal tissue repair process according to the employed concentration. |
| first_indexed | 2024-04-13T06:02:00Z |
| format | Article |
| id | doaj.art-0780828b3f5748c0a25b6ca1007991df |
| institution | Directory Open Access Journal |
| issn | 1013-9052 |
| language | English |
| last_indexed | 2024-04-13T06:02:00Z |
| publishDate | 2022-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Saudi Dental Journal |
| spelling | doaj.art-0780828b3f5748c0a25b6ca1007991df2022-12-22T02:59:24ZengElsevierSaudi Dental Journal1013-90522022-09-01346516526The effects of Lactobacillus reuteri on the inflammation and periodontal tissue repair in rats: A pilot studyValdir Gouveia Garcia0Daniela Maria Janjácomo Miessi1Tiago Esgalha da Rocha2Natália Amanda Gomes3Marta Aparecida Alberton Nuernberg4Jânderson de Medeiros Cardoso5Edilson Ervolino6Letícia Helena Theodoro7Periodontics Division, Department of Diagnostic and Surgery, School of Dentistry of Araçatuba, São Paulo State University (UNESP), Araçatuba, SP, Brazil; Latin American Institute of Dental Research and Education (ILAPEO), Curitiba, PR, BrazilPeriodontics Division, Department of Diagnostic and Surgery, School of Dentistry of Araçatuba, São Paulo State University (UNESP), Araçatuba, SP, BrazilPeriodontics Division, Department of Diagnostic and Surgery, School of Dentistry of Araçatuba, São Paulo State University (UNESP), Araçatuba, SP, BrazilPeriodontics Division, Department of Diagnostic and Surgery, School of Dentistry of Araçatuba, São Paulo State University (UNESP), Araçatuba, SP, BrazilPeriodontics Division, Department of Diagnostic and Surgery, School of Dentistry of Araçatuba, São Paulo State University (UNESP), Araçatuba, SP, BrazilLatin American Institute of Dental Research and Education (ILAPEO), Curitiba, PR, BrazilDepartment of Basic Sciences, School of Dentistry of Araçatuba, São Paulo State University (UNESP), Araçatuba, SP, BrazilPeriodontics Division, Department of Diagnostic and Surgery, School of Dentistry of Araçatuba, São Paulo State University (UNESP), Araçatuba, SP, Brazil; Corresponding author at: Faculdade de Odontologia de Araçatuba-UNESP, Rua José Bonifácio, 1193, Centro, 16015-050 Araçatuba, SP, Brazil.Objective: The aim of this study was to evaluate the effects of probiotic (PRO) Lactobacillus reuteri (DSM17938) as an adjuvant to the treatment of experimental periodontitis (EP). Material and methods: Fifty-four male adult Wistar rats were included. EP was induced and maintained for 7 days. Subsequently, the ligature was removed and the animals were allocated into three different experimental groups (n = 18/group): EP – no local treatment, the animals received four systemic saline solution (SS) administrations; SRP+SS, the animals underwent SRP treatment, followed by SS administration; and SRP+PRO, the animals received SRP treatment, followed by the systemic administration of PROs (Lactobacillus reuteri ; 0.16 ml/day). Six animals from each group were euthanised at 7, 15 and 30 days. Histological and histometric analyses of alveolar bone loss (BL) and immunohistochemical analyses for TRAP, RANKL, OPG, OCN, and PCNA were performed. Shapiro–Wilk, ANOVA, post-hoc Tukey, Kruskal–Wallis, Student–Newman Keuls were performed. Results: The SRP+PRO group presented a reduction in inflammation. At 15 days, a lower BL was observed in the SRP+SS and SRP+PRO groups. Greater immunolabeling was noticed for PCNA at 15 days in the SRP+PRO group than in the SRP+SS group. The SRP+PRO group demonstrated a higher OCN immunolabeling pattern than the EP group at 15 and 30 days. Conclusion: The use of Lactobacillus reuteri as an adjuvant to SRP for the treatment of EP showed promising results in the control of local inflammatory responses, and enhanced the periodontal tissue repair process according to the employed concentration.http://www.sciencedirect.com/science/article/pii/S1013905222000608DentistryPeriodontologyLactobacillus reuteriPeriodontal diseaseProbiotic |
| spellingShingle | Valdir Gouveia Garcia Daniela Maria Janjácomo Miessi Tiago Esgalha da Rocha Natália Amanda Gomes Marta Aparecida Alberton Nuernberg Jânderson de Medeiros Cardoso Edilson Ervolino Letícia Helena Theodoro The effects of Lactobacillus reuteri on the inflammation and periodontal tissue repair in rats: A pilot study Saudi Dental Journal Dentistry Periodontology Lactobacillus reuteri Periodontal disease Probiotic |
| title | The effects of Lactobacillus reuteri on the inflammation and periodontal tissue repair in rats: A pilot study |
| title_full | The effects of Lactobacillus reuteri on the inflammation and periodontal tissue repair in rats: A pilot study |
| title_fullStr | The effects of Lactobacillus reuteri on the inflammation and periodontal tissue repair in rats: A pilot study |
| title_full_unstemmed | The effects of Lactobacillus reuteri on the inflammation and periodontal tissue repair in rats: A pilot study |
| title_short | The effects of Lactobacillus reuteri on the inflammation and periodontal tissue repair in rats: A pilot study |
| title_sort | effects of lactobacillus reuteri on the inflammation and periodontal tissue repair in rats a pilot study |
| topic | Dentistry Periodontology Lactobacillus reuteri Periodontal disease Probiotic |
| url | http://www.sciencedirect.com/science/article/pii/S1013905222000608 |
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