Dynamic changes in virus-induced volatiles in cotton modulate the orientation and oviposition behavior of the whitefly Bemisia tabaci
Manipulation of insect vector behavior by virus-induced plant volatiles is well known. But how the viral disease progression alters the plant volatiles and its effect on vector behavior remains less explored. Our studies tracked changes in volatile profile in progressive infection stages of cotton l...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2022-10-01
|
| Series: | Frontiers in Physiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fphys.2022.1017948/full |
| _version_ | 1798020915679723520 |
|---|---|
| author | Suresh M. Nebapure Karuppan Shankarganesh Karuppan Shankarganesh Salim Rajna Kailash Chandra Naga Dheerendra Pandey Shubham Gambhir Koovalamkadu Velayudhan Praveen Sabtharishi Subramanian |
| author_facet | Suresh M. Nebapure Karuppan Shankarganesh Karuppan Shankarganesh Salim Rajna Kailash Chandra Naga Dheerendra Pandey Shubham Gambhir Koovalamkadu Velayudhan Praveen Sabtharishi Subramanian |
| author_sort | Suresh M. Nebapure |
| collection | DOAJ |
| description | Manipulation of insect vector behavior by virus-induced plant volatiles is well known. But how the viral disease progression alters the plant volatiles and its effect on vector behavior remains less explored. Our studies tracked changes in volatile profile in progressive infection stages of cotton leaf curl virus (CLCuV) infected plants and their effect on B. tabaci behavior. Significant differences in virus titers were noticed between progressive infection stages showing distinct symptoms. Whiteflies initially settled on CLCuV infected plants, but their preference was shifted to healthy plants over time. GC-MS analysis revealed subtle quantitative/qualitative changes in volatile organic compounds (VOCs) between the healthy and selected CLCuV infection stages. VOCs such as hexanal, (E)-2-hexen-1-ol, (+)-α-pinene, (−)-β-pinene, (Z)-3-hexen-1-ol, (+)-sylvestrene, and (1S,2E,6E, 10R)-3,7,11,11-tetramethylbicycloundeca-2,6-diene (Bicyclogermacrene) were associated with the infection stage showing upward curling of leaves; (E)-2-hexen-1-ol, β-myrcene, β-ocimene, and copaene were associated with the infection stage showing downward curling. Validation studies with eight synthetic VOCs indicated that γ-terpinene elicited attraction to B. tabaci (Olfactometric Preference Index (OPI) = 1.65), while β-ocimene exhibited strong repellence (OPI = 0.64) and oviposition reduction (66.01%–92.55%). Our studies have demonstrated that progression of CLCuV disease in cotton was associated with dynamic changes in volatile profile which influences the behavioural responses of whitefly, B.tabaci. Results have shown that VOCs such as (+)-α-pinene, (−)-β-pinene γ-Terpinene, α-guaiene; 4- hydroxy- 4 methyl-2- pentanone and β-ocimene emitted from Begomovirus infected plants could be the driving force for early attraction and later repellence/oviposition deterrence of B. tabaci on virus-infected plants. The findings of this study offer scope for the management of whitefly, B. tabaci through semiochemicals. |
| first_indexed | 2024-04-11T17:05:27Z |
| format | Article |
| id | doaj.art-078604e39c0d4d31a10d5e4484ab2800 |
| institution | Directory Open Access Journal |
| issn | 1664-042X |
| language | English |
| last_indexed | 2024-04-11T17:05:27Z |
| publishDate | 2022-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Physiology |
| spelling | doaj.art-078604e39c0d4d31a10d5e4484ab28002022-12-22T04:13:03ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2022-10-011310.3389/fphys.2022.10179481017948Dynamic changes in virus-induced volatiles in cotton modulate the orientation and oviposition behavior of the whitefly Bemisia tabaciSuresh M. Nebapure0Karuppan Shankarganesh1Karuppan Shankarganesh2Salim Rajna3Kailash Chandra Naga4Dheerendra Pandey5Shubham Gambhir6Koovalamkadu Velayudhan Praveen7Sabtharishi Subramanian8ICAR-Indian Agricultural Research Institute, New Delhi, IndiaICAR-Indian Agricultural Research Institute, New Delhi, IndiaICAR-Central Institute for Cotton Research, Regional Station, Coimbatore, IndiaICAR-Indian Agricultural Research Institute, New Delhi, IndiaICAR-Central Potato Research Institute, Shimla, IndiaICAR-Indian Agricultural Research Institute, New Delhi, IndiaICAR-Indian Agricultural Research Institute, New Delhi, IndiaICAR-Indian Agricultural Research Institute, New Delhi, IndiaICAR-Indian Agricultural Research Institute, New Delhi, IndiaManipulation of insect vector behavior by virus-induced plant volatiles is well known. But how the viral disease progression alters the plant volatiles and its effect on vector behavior remains less explored. Our studies tracked changes in volatile profile in progressive infection stages of cotton leaf curl virus (CLCuV) infected plants and their effect on B. tabaci behavior. Significant differences in virus titers were noticed between progressive infection stages showing distinct symptoms. Whiteflies initially settled on CLCuV infected plants, but their preference was shifted to healthy plants over time. GC-MS analysis revealed subtle quantitative/qualitative changes in volatile organic compounds (VOCs) between the healthy and selected CLCuV infection stages. VOCs such as hexanal, (E)-2-hexen-1-ol, (+)-α-pinene, (−)-β-pinene, (Z)-3-hexen-1-ol, (+)-sylvestrene, and (1S,2E,6E, 10R)-3,7,11,11-tetramethylbicycloundeca-2,6-diene (Bicyclogermacrene) were associated with the infection stage showing upward curling of leaves; (E)-2-hexen-1-ol, β-myrcene, β-ocimene, and copaene were associated with the infection stage showing downward curling. Validation studies with eight synthetic VOCs indicated that γ-terpinene elicited attraction to B. tabaci (Olfactometric Preference Index (OPI) = 1.65), while β-ocimene exhibited strong repellence (OPI = 0.64) and oviposition reduction (66.01%–92.55%). Our studies have demonstrated that progression of CLCuV disease in cotton was associated with dynamic changes in volatile profile which influences the behavioural responses of whitefly, B.tabaci. Results have shown that VOCs such as (+)-α-pinene, (−)-β-pinene γ-Terpinene, α-guaiene; 4- hydroxy- 4 methyl-2- pentanone and β-ocimene emitted from Begomovirus infected plants could be the driving force for early attraction and later repellence/oviposition deterrence of B. tabaci on virus-infected plants. The findings of this study offer scope for the management of whitefly, B. tabaci through semiochemicals.https://www.frontiersin.org/articles/10.3389/fphys.2022.1017948/fullwhiteflycotton leaf curl virusinteractionsvolatile organic compoundsovipositionorientation |
| spellingShingle | Suresh M. Nebapure Karuppan Shankarganesh Karuppan Shankarganesh Salim Rajna Kailash Chandra Naga Dheerendra Pandey Shubham Gambhir Koovalamkadu Velayudhan Praveen Sabtharishi Subramanian Dynamic changes in virus-induced volatiles in cotton modulate the orientation and oviposition behavior of the whitefly Bemisia tabaci Frontiers in Physiology whitefly cotton leaf curl virus interactions volatile organic compounds oviposition orientation |
| title | Dynamic changes in virus-induced volatiles in cotton modulate the orientation and oviposition behavior of the whitefly Bemisia tabaci |
| title_full | Dynamic changes in virus-induced volatiles in cotton modulate the orientation and oviposition behavior of the whitefly Bemisia tabaci |
| title_fullStr | Dynamic changes in virus-induced volatiles in cotton modulate the orientation and oviposition behavior of the whitefly Bemisia tabaci |
| title_full_unstemmed | Dynamic changes in virus-induced volatiles in cotton modulate the orientation and oviposition behavior of the whitefly Bemisia tabaci |
| title_short | Dynamic changes in virus-induced volatiles in cotton modulate the orientation and oviposition behavior of the whitefly Bemisia tabaci |
| title_sort | dynamic changes in virus induced volatiles in cotton modulate the orientation and oviposition behavior of the whitefly bemisia tabaci |
| topic | whitefly cotton leaf curl virus interactions volatile organic compounds oviposition orientation |
| url | https://www.frontiersin.org/articles/10.3389/fphys.2022.1017948/full |
| work_keys_str_mv | AT sureshmnebapure dynamicchangesinvirusinducedvolatilesincottonmodulatetheorientationandovipositionbehaviorofthewhiteflybemisiatabaci AT karuppanshankarganesh dynamicchangesinvirusinducedvolatilesincottonmodulatetheorientationandovipositionbehaviorofthewhiteflybemisiatabaci AT karuppanshankarganesh dynamicchangesinvirusinducedvolatilesincottonmodulatetheorientationandovipositionbehaviorofthewhiteflybemisiatabaci AT salimrajna dynamicchangesinvirusinducedvolatilesincottonmodulatetheorientationandovipositionbehaviorofthewhiteflybemisiatabaci AT kailashchandranaga dynamicchangesinvirusinducedvolatilesincottonmodulatetheorientationandovipositionbehaviorofthewhiteflybemisiatabaci AT dheerendrapandey dynamicchangesinvirusinducedvolatilesincottonmodulatetheorientationandovipositionbehaviorofthewhiteflybemisiatabaci AT shubhamgambhir dynamicchangesinvirusinducedvolatilesincottonmodulatetheorientationandovipositionbehaviorofthewhiteflybemisiatabaci AT koovalamkaduvelayudhanpraveen dynamicchangesinvirusinducedvolatilesincottonmodulatetheorientationandovipositionbehaviorofthewhiteflybemisiatabaci AT sabtharishisubramanian dynamicchangesinvirusinducedvolatilesincottonmodulatetheorientationandovipositionbehaviorofthewhiteflybemisiatabaci |