A sensitive serodiagnostic tool for the detection of active infection of zoonotic visceral and nasopharyngeal linguatulosis
Aim: This study aimed to evaluate the different serological techniques for early diagnosis of acute concurrent infections with linguatulosis in the definitive host (dogs) and an intermediate host (goats). This evaluation compared between the gold standard (GS) test (GS; examination of nasal and feca...
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| Format: | Article |
| Language: | English |
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Veterinary World
2019-06-01
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| Series: | Veterinary World |
| Subjects: | |
| Online Access: | http://www.veterinaryworld.org/Vol.12/June-2019/24.pdf |
| _version_ | 1819128538020708352 |
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| author | Marwa M. Attia Elshaimaa Ismael Nagla M. K. Saleh |
| author_facet | Marwa M. Attia Elshaimaa Ismael Nagla M. K. Saleh |
| author_sort | Marwa M. Attia |
| collection | DOAJ |
| description | Aim: This study aimed to evaluate the different serological techniques for early diagnosis of acute concurrent infections with linguatulosis in the definitive host (dogs) and an intermediate host (goats). This evaluation compared between the gold standard (GS) test (GS; examination of nasal and fecal samples in dogs and examination of lymph nodes in goats), sandwich enzyme-linked immunosorbent assay (S-ELISA), and indirect ELISA.
Materials and Methods: Fifty goats and fifty dogs were examined for the presence of Linguatula serrata nymphs and adults, respectively, besides the collection of blood samples from the examined animals for serologic testing.
Results: In goats; GS, S-ELISA, and indirect ELISA showed positivity in 32 (64%), 28 (56%), and 39 (78%) samples, respectively. In dogs; GS, S-ELISA, and indirect ELISA showed positivity in 25 (50%), 25 (50%), and 30 (60%) samples, respectively. S-ELISA displayed significant higher agreement with the GS test (≥0.83) than indirect ELISA (≤0.67) in both hosts. Infection with linguatulosis showed significant relation with the age of goats and dogs and the sex of goats (p<0.05).
Conclusion: S-ELISA displayed more sensitivity and specificity for the detection of concurrent infections with linguatulosis in both dogs and goats than indirect ELISA, which could detect the prior infections. Similarly, these assays could be used for diagnosis of concurrent infections with linguatulosis in human, especially the chronic ones. |
| first_indexed | 2024-12-22T08:29:24Z |
| format | Article |
| id | doaj.art-078a160985ba43bd9a60637bdb387449 |
| institution | Directory Open Access Journal |
| issn | 0972-8988 2231-0916 |
| language | English |
| last_indexed | 2024-12-22T08:29:24Z |
| publishDate | 2019-06-01 |
| publisher | Veterinary World |
| record_format | Article |
| series | Veterinary World |
| spelling | doaj.art-078a160985ba43bd9a60637bdb3874492022-12-21T18:32:32ZengVeterinary WorldVeterinary World0972-89882231-09162019-06-0112688388910.14202/vetworld.2019.883-889A sensitive serodiagnostic tool for the detection of active infection of zoonotic visceral and nasopharyngeal linguatulosisMarwa M. Attia0Elshaimaa Ismael1Nagla M. K. Saleh2Department of Parasitology, Faculty of Veterinary Medicine, Cairo University, Giza 12211, Egypt.Department of Veterinary Hygiene and Management, Faculty of Veterinary Medicine, Cairo University, Giza 12211, Egypt.Department of Zoology, Faculty of Science, Aswan University, Aswan, Egypt.Aim: This study aimed to evaluate the different serological techniques for early diagnosis of acute concurrent infections with linguatulosis in the definitive host (dogs) and an intermediate host (goats). This evaluation compared between the gold standard (GS) test (GS; examination of nasal and fecal samples in dogs and examination of lymph nodes in goats), sandwich enzyme-linked immunosorbent assay (S-ELISA), and indirect ELISA. Materials and Methods: Fifty goats and fifty dogs were examined for the presence of Linguatula serrata nymphs and adults, respectively, besides the collection of blood samples from the examined animals for serologic testing. Results: In goats; GS, S-ELISA, and indirect ELISA showed positivity in 32 (64%), 28 (56%), and 39 (78%) samples, respectively. In dogs; GS, S-ELISA, and indirect ELISA showed positivity in 25 (50%), 25 (50%), and 30 (60%) samples, respectively. S-ELISA displayed significant higher agreement with the GS test (≥0.83) than indirect ELISA (≤0.67) in both hosts. Infection with linguatulosis showed significant relation with the age of goats and dogs and the sex of goats (p<0.05). Conclusion: S-ELISA displayed more sensitivity and specificity for the detection of concurrent infections with linguatulosis in both dogs and goats than indirect ELISA, which could detect the prior infections. Similarly, these assays could be used for diagnosis of concurrent infections with linguatulosis in human, especially the chronic ones.http://www.veterinaryworld.org/Vol.12/June-2019/24.pdfhalzounindirect enzyme-linked immunosorbent assayLinguatula serratasandwich enzyme-linked immunosorbent assayvisceral linguatulosis |
| spellingShingle | Marwa M. Attia Elshaimaa Ismael Nagla M. K. Saleh A sensitive serodiagnostic tool for the detection of active infection of zoonotic visceral and nasopharyngeal linguatulosis Veterinary World halzoun indirect enzyme-linked immunosorbent assay Linguatula serrata sandwich enzyme-linked immunosorbent assay visceral linguatulosis |
| title | A sensitive serodiagnostic tool for the detection of active infection of zoonotic visceral and nasopharyngeal linguatulosis |
| title_full | A sensitive serodiagnostic tool for the detection of active infection of zoonotic visceral and nasopharyngeal linguatulosis |
| title_fullStr | A sensitive serodiagnostic tool for the detection of active infection of zoonotic visceral and nasopharyngeal linguatulosis |
| title_full_unstemmed | A sensitive serodiagnostic tool for the detection of active infection of zoonotic visceral and nasopharyngeal linguatulosis |
| title_short | A sensitive serodiagnostic tool for the detection of active infection of zoonotic visceral and nasopharyngeal linguatulosis |
| title_sort | sensitive serodiagnostic tool for the detection of active infection of zoonotic visceral and nasopharyngeal linguatulosis |
| topic | halzoun indirect enzyme-linked immunosorbent assay Linguatula serrata sandwich enzyme-linked immunosorbent assay visceral linguatulosis |
| url | http://www.veterinaryworld.org/Vol.12/June-2019/24.pdf |
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