An i-type lysozyme (CfLyzI) involved in innate immunity is essential for the survival of Chlamys farreri during Vibrio stimulation
Lysozymes act as key components of the innate immunity in invertebrates and play a pivotal role in early defense against invading microbe infection. In this study, an i-type lysozyme homology was identified and characterized in Zhikong scallop Chlamys farreri (designated as CfLyzI). The full-length...
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| Format: | Article |
| Language: | English |
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University of Modena and Reggio Emilia
2018-08-01
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| Series: | Invertebrate Survival Journal |
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| Online Access: | https://isj02.unimore.it/index.php/ISJ/article/view/434 |
| _version_ | 1818965667892690944 |
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| author | M Q Wang B J Wang M Liu K Y Jiang L Wang |
| author_facet | M Q Wang B J Wang M Liu K Y Jiang L Wang |
| author_sort | M Q Wang |
| collection | DOAJ |
| description | Lysozymes act as key components of the innate immunity in invertebrates and play a pivotal role in early defense against invading microbe infection. In this study, an i-type lysozyme homology was identified and characterized in Zhikong scallop Chlamys farreri (designated as CfLyzI). The full-length cDNA sequence of CfLyzI contained a 702 bp open reading frame (ORF), which encoded a polypeptide of 233 amino acids and contained a typical destabilase function domain. The mRNA transcripts of CfLyzI were detectable in all the investigated tissues, including hemocytes, muscle, mantle, gill, hepatopancreas and gonad with the peak level in hemocytes. Being stimulated by Vibrio splendidus, the mRNA transcripts of CfLyzI significantly increased in hemocytes. The CfLyzI-suppressed scallops turned to be more susceptible to Vibrio. All these results indicated that CfLyzI acted as an efficient effector in the innate immunity and was also essential for hosts’ survival during Vibrio stimulation in Zhikong scallop. |
| first_indexed | 2024-12-20T13:20:39Z |
| format | Article |
| id | doaj.art-078b63307db8470eaf3182eadb5cca10 |
| institution | Directory Open Access Journal |
| issn | 1824-307X |
| language | English |
| last_indexed | 2024-12-20T13:20:39Z |
| publishDate | 2018-08-01 |
| publisher | University of Modena and Reggio Emilia |
| record_format | Article |
| series | Invertebrate Survival Journal |
| spelling | doaj.art-078b63307db8470eaf3182eadb5cca102022-12-21T19:39:24ZengUniversity of Modena and Reggio EmiliaInvertebrate Survival Journal1824-307X2018-08-01151An i-type lysozyme (CfLyzI) involved in innate immunity is essential for the survival of Chlamys farreri during Vibrio stimulationM Q Wang0B J Wang1M Liu2K Y Jiang3L Wang4CAS Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China ; Research Platform for Marine Molecular Biotechnology, Pilot National Laboratory for Marine Science and Technology, Qingdao 266237, ChinaCAS Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, ChinaCAS Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, ChinaCAS Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, ChinaCAS Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China ; Laboratory for Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology, Qingdao 266237, China ; Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao 266400, ChinaLysozymes act as key components of the innate immunity in invertebrates and play a pivotal role in early defense against invading microbe infection. In this study, an i-type lysozyme homology was identified and characterized in Zhikong scallop Chlamys farreri (designated as CfLyzI). The full-length cDNA sequence of CfLyzI contained a 702 bp open reading frame (ORF), which encoded a polypeptide of 233 amino acids and contained a typical destabilase function domain. The mRNA transcripts of CfLyzI were detectable in all the investigated tissues, including hemocytes, muscle, mantle, gill, hepatopancreas and gonad with the peak level in hemocytes. Being stimulated by Vibrio splendidus, the mRNA transcripts of CfLyzI significantly increased in hemocytes. The CfLyzI-suppressed scallops turned to be more susceptible to Vibrio. All these results indicated that CfLyzI acted as an efficient effector in the innate immunity and was also essential for hosts’ survival during Vibrio stimulation in Zhikong scallop.https://isj02.unimore.it/index.php/ISJ/article/view/434Chlamys farreriInnate immunityInvertebrate type lysozyme |
| spellingShingle | M Q Wang B J Wang M Liu K Y Jiang L Wang An i-type lysozyme (CfLyzI) involved in innate immunity is essential for the survival of Chlamys farreri during Vibrio stimulation Invertebrate Survival Journal Chlamys farreri Innate immunity Invertebrate type lysozyme |
| title | An i-type lysozyme (CfLyzI) involved in innate immunity is essential for the survival of Chlamys farreri during Vibrio stimulation |
| title_full | An i-type lysozyme (CfLyzI) involved in innate immunity is essential for the survival of Chlamys farreri during Vibrio stimulation |
| title_fullStr | An i-type lysozyme (CfLyzI) involved in innate immunity is essential for the survival of Chlamys farreri during Vibrio stimulation |
| title_full_unstemmed | An i-type lysozyme (CfLyzI) involved in innate immunity is essential for the survival of Chlamys farreri during Vibrio stimulation |
| title_short | An i-type lysozyme (CfLyzI) involved in innate immunity is essential for the survival of Chlamys farreri during Vibrio stimulation |
| title_sort | i type lysozyme cflyzi involved in innate immunity is essential for the survival of chlamys farreri during vibrio stimulation |
| topic | Chlamys farreri Innate immunity Invertebrate type lysozyme |
| url | https://isj02.unimore.it/index.php/ISJ/article/view/434 |
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