Novel Ablation Therapy Using Endoscopic Irreversible Electroporation in the Bile Duct: A Pilot Animal Study
Background/Aims Irreversible electroporation (IRE) is a relatively new ablation method. However, the application of IRE ablation in the treatment of biliary disease has not been attempted. A minimally invasive approach using endoscopic retrograde cholangiopancreatography (ERCP) can be a novel therap...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Korean Society of Gastrointestinal Endoscopy
2021-05-01
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| Series: | Clinical Endoscopy |
| Subjects: | |
| Online Access: | http://www.e-ce.org/upload/pdf/ce-2020-126.pdf |
| _version_ | 1797427471977545728 |
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| author | Kang Won Lee Jae Min Lee Hyuk Soon Choi Eun Sun Kim Bora Keum Yeon Seok Seo Yoon Tae Jeen Soon Ho Um Hong Sik Lee Hoon Jai Chun Chang Duck Kim Chi Hyuk Oh Hong Bae Kim |
| author_facet | Kang Won Lee Jae Min Lee Hyuk Soon Choi Eun Sun Kim Bora Keum Yeon Seok Seo Yoon Tae Jeen Soon Ho Um Hong Sik Lee Hoon Jai Chun Chang Duck Kim Chi Hyuk Oh Hong Bae Kim |
| author_sort | Kang Won Lee |
| collection | DOAJ |
| description | Background/Aims Irreversible electroporation (IRE) is a relatively new ablation method. However, the application of IRE ablation in the treatment of biliary disease has not been attempted. A minimally invasive approach using endoscopic retrograde cholangiopancreatography (ERCP) can be a novel therapeutic modality for IRE ablation. In this study, we aimed to investigate the feasibility of endoscopic IRE for the biliary tract using an animal model. Methods A new catheter-type electrode was developed for endoscopic IRE ablation of the biliary tract. We performed ERCP and endoscopic IRE ablations in the normal common bile duct of Yorkshire pigs. The experimental setting of IRE was 500 V/cm (50 pulses, 100-µs length). The animals were sacrificed after 24 hr, and the ablated bile duct was examined. Results Well-demarcated focal color changes were observed on the mucosa of the common bile duct. The depth of change after IRE was confined to the mucosal and submucosal layers. Apoptotic changes in the bile duct were observed only around the IRE ablation area. Immunohistochemistry assay showed cell death in the bile duct along the electrode. Conclusions Endoscopic IRE ablation using ERCP was successfully performed in the common bile duct. It can be a potential option for the treatment of biliary tumors. |
| first_indexed | 2024-03-09T08:44:32Z |
| format | Article |
| id | doaj.art-079509bba12142c9ac341aea911c4a08 |
| institution | Directory Open Access Journal |
| issn | 2234-2400 2234-2443 |
| language | English |
| last_indexed | 2024-03-09T08:44:32Z |
| publishDate | 2021-05-01 |
| publisher | Korean Society of Gastrointestinal Endoscopy |
| record_format | Article |
| series | Clinical Endoscopy |
| spelling | doaj.art-079509bba12142c9ac341aea911c4a082023-12-02T15:38:59ZengKorean Society of Gastrointestinal EndoscopyClinical Endoscopy2234-24002234-24432021-05-0154341341910.5946/ce.2020.1267416Novel Ablation Therapy Using Endoscopic Irreversible Electroporation in the Bile Duct: A Pilot Animal StudyKang Won Lee0Jae Min Lee1Hyuk Soon Choi2Eun Sun Kim3Bora Keum4Yeon Seok Seo5Yoon Tae Jeen6Soon Ho Um7Hong Sik Lee8Hoon Jai Chun9Chang Duck Kim10Chi Hyuk Oh11Hong Bae Kim12 Division of Gastroenterology and Hepatology, Department of Internal Medicine, Korea University College of Medicine, Seoul, Korea Division of Gastroenterology and Hepatology, Department of Internal Medicine, Korea University College of Medicine, Seoul, Korea Division of Gastroenterology and Hepatology, Department of Internal Medicine, Korea University College of Medicine, Seoul, Korea Division of Gastroenterology and Hepatology, Department of Internal Medicine, Korea University College of Medicine, Seoul, Korea Division of Gastroenterology and Hepatology, Department of Internal Medicine, Korea University College of Medicine, Seoul, Korea Division of Gastroenterology and Hepatology, Department of Internal Medicine, Korea University College of Medicine, Seoul, Korea Division of Gastroenterology and Hepatology, Department of Internal Medicine, Korea University College of Medicine, Seoul, Korea Division of Gastroenterology and Hepatology, Department of Internal Medicine, Korea University College of Medicine, Seoul, Korea Division of Gastroenterology and Hepatology, Department of Internal Medicine, Korea University College of Medicine, Seoul, Korea Division of Gastroenterology and Hepatology, Department of Internal Medicine, Korea University College of Medicine, Seoul, Korea Division of Gastroenterology and Hepatology, Department of Internal Medicine, Korea University College of Medicine, Seoul, Korea Division of Gastroenterology and Hepatology, Department of Internal Medicine, Kyung Hee University College of Medicine, Seoul, Korea Department of Biosystems and Biomaterials Science and Engineering, Seoul National University, Seoul, KoreaBackground/Aims Irreversible electroporation (IRE) is a relatively new ablation method. However, the application of IRE ablation in the treatment of biliary disease has not been attempted. A minimally invasive approach using endoscopic retrograde cholangiopancreatography (ERCP) can be a novel therapeutic modality for IRE ablation. In this study, we aimed to investigate the feasibility of endoscopic IRE for the biliary tract using an animal model. Methods A new catheter-type electrode was developed for endoscopic IRE ablation of the biliary tract. We performed ERCP and endoscopic IRE ablations in the normal common bile duct of Yorkshire pigs. The experimental setting of IRE was 500 V/cm (50 pulses, 100-µs length). The animals were sacrificed after 24 hr, and the ablated bile duct was examined. Results Well-demarcated focal color changes were observed on the mucosa of the common bile duct. The depth of change after IRE was confined to the mucosal and submucosal layers. Apoptotic changes in the bile duct were observed only around the IRE ablation area. Immunohistochemistry assay showed cell death in the bile duct along the electrode. Conclusions Endoscopic IRE ablation using ERCP was successfully performed in the common bile duct. It can be a potential option for the treatment of biliary tumors.http://www.e-ce.org/upload/pdf/ce-2020-126.pdfablationbile ductendoscopic retrograde cholangiopancreatographyirreversible electroporation |
| spellingShingle | Kang Won Lee Jae Min Lee Hyuk Soon Choi Eun Sun Kim Bora Keum Yeon Seok Seo Yoon Tae Jeen Soon Ho Um Hong Sik Lee Hoon Jai Chun Chang Duck Kim Chi Hyuk Oh Hong Bae Kim Novel Ablation Therapy Using Endoscopic Irreversible Electroporation in the Bile Duct: A Pilot Animal Study Clinical Endoscopy ablation bile duct endoscopic retrograde cholangiopancreatography irreversible electroporation |
| title | Novel Ablation Therapy Using Endoscopic Irreversible Electroporation in the Bile Duct: A Pilot Animal Study |
| title_full | Novel Ablation Therapy Using Endoscopic Irreversible Electroporation in the Bile Duct: A Pilot Animal Study |
| title_fullStr | Novel Ablation Therapy Using Endoscopic Irreversible Electroporation in the Bile Duct: A Pilot Animal Study |
| title_full_unstemmed | Novel Ablation Therapy Using Endoscopic Irreversible Electroporation in the Bile Duct: A Pilot Animal Study |
| title_short | Novel Ablation Therapy Using Endoscopic Irreversible Electroporation in the Bile Duct: A Pilot Animal Study |
| title_sort | novel ablation therapy using endoscopic irreversible electroporation in the bile duct a pilot animal study |
| topic | ablation bile duct endoscopic retrograde cholangiopancreatography irreversible electroporation |
| url | http://www.e-ce.org/upload/pdf/ce-2020-126.pdf |
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