Comparative Analysis of Age- and Gender-Associated Microbiome in Lung Adenocarcinoma and Lung Squamous Cell Carcinoma
The intra-tumor microbiota has been increasingly implicated in cancer pathogenesis. In this study, we aimed to examine the microbiome in lung adenocarcinoma (LUAD) and lung squamous cell carcinoma (LUSC) and determine its compositional differences with relation to age and gender. After grouping 497...
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| Format: | Article |
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MDPI AG
2020-06-01
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| Series: | Cancers |
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| Online Access: | https://www.mdpi.com/2072-6694/12/6/1447 |
| _version_ | 1797566274781315072 |
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| author | Lindsay M. Wong Neil Shende Wei Tse Li Grant Castaneda Lauren Apostol Eric Y. Chang Weg M. Ongkeko |
| author_facet | Lindsay M. Wong Neil Shende Wei Tse Li Grant Castaneda Lauren Apostol Eric Y. Chang Weg M. Ongkeko |
| author_sort | Lindsay M. Wong |
| collection | DOAJ |
| description | The intra-tumor microbiota has been increasingly implicated in cancer pathogenesis. In this study, we aimed to examine the microbiome in lung adenocarcinoma (LUAD) and lung squamous cell carcinoma (LUSC) and determine its compositional differences with relation to age and gender. After grouping 497 LUAD and 433 LUSC patients by age and gender and removing potential contaminants, we identified differentially abundant microbes in each patient cohort vs. adjacent normal samples. We then correlated dysregulated microbes with patient survival rates, immune infiltration, immune and cancer pathways, and genomic alterations. We found that most age and gender cohorts in both LUAD and LUSC contained unique, significantly dysregulated microbes. For example, LUAD-associated <i>Escherichia coli</i> str. K-12 substr. W3110 was dysregulated in older female and male patients and correlated with both patient survival and genomic alterations. For LUSC, the most prominent bacterial species that we identified was <i>Pseudomonas putida</i> str. KT2440, which was uniquely associated with young LUSC male patients and immune infiltration. In conclusion, we found differentially abundant microbes implicated with age and gender that are also associated with genomic alterations and immune dysregulations. Further investigation should be conducted to determine the relationship between gender and age-associated microbes and the pathogenesis of lung cancer. |
| first_indexed | 2024-03-10T19:25:21Z |
| format | Article |
| id | doaj.art-0795ee3261a145798e5ddae7e915c9f4 |
| institution | Directory Open Access Journal |
| issn | 2072-6694 |
| language | English |
| last_indexed | 2024-03-10T19:25:21Z |
| publishDate | 2020-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cancers |
| spelling | doaj.art-0795ee3261a145798e5ddae7e915c9f42023-11-20T02:36:09ZengMDPI AGCancers2072-66942020-06-01126144710.3390/cancers12061447Comparative Analysis of Age- and Gender-Associated Microbiome in Lung Adenocarcinoma and Lung Squamous Cell CarcinomaLindsay M. Wong0Neil Shende1Wei Tse Li2Grant Castaneda3Lauren Apostol4Eric Y. Chang5Weg M. Ongkeko6Department of Otolaryngology-Head and Neck Surgery, University of California, La Jolla, San Diego, CA 92093, USADepartment of Otolaryngology-Head and Neck Surgery, University of California, La Jolla, San Diego, CA 92093, USADepartment of Otolaryngology-Head and Neck Surgery, University of California, La Jolla, San Diego, CA 92093, USADepartment of Otolaryngology-Head and Neck Surgery, University of California, La Jolla, San Diego, CA 92093, USADepartment of Otolaryngology-Head and Neck Surgery, University of California, La Jolla, San Diego, CA 92093, USADepartment of Radiology, University of California, San Diego, CA 92093, USADepartment of Otolaryngology-Head and Neck Surgery, University of California, La Jolla, San Diego, CA 92093, USAThe intra-tumor microbiota has been increasingly implicated in cancer pathogenesis. In this study, we aimed to examine the microbiome in lung adenocarcinoma (LUAD) and lung squamous cell carcinoma (LUSC) and determine its compositional differences with relation to age and gender. After grouping 497 LUAD and 433 LUSC patients by age and gender and removing potential contaminants, we identified differentially abundant microbes in each patient cohort vs. adjacent normal samples. We then correlated dysregulated microbes with patient survival rates, immune infiltration, immune and cancer pathways, and genomic alterations. We found that most age and gender cohorts in both LUAD and LUSC contained unique, significantly dysregulated microbes. For example, LUAD-associated <i>Escherichia coli</i> str. K-12 substr. W3110 was dysregulated in older female and male patients and correlated with both patient survival and genomic alterations. For LUSC, the most prominent bacterial species that we identified was <i>Pseudomonas putida</i> str. KT2440, which was uniquely associated with young LUSC male patients and immune infiltration. In conclusion, we found differentially abundant microbes implicated with age and gender that are also associated with genomic alterations and immune dysregulations. Further investigation should be conducted to determine the relationship between gender and age-associated microbes and the pathogenesis of lung cancer.https://www.mdpi.com/2072-6694/12/6/1447lung adenocarcinomalung squamous cell carcinomamicrobiomeagegenderTCGA |
| spellingShingle | Lindsay M. Wong Neil Shende Wei Tse Li Grant Castaneda Lauren Apostol Eric Y. Chang Weg M. Ongkeko Comparative Analysis of Age- and Gender-Associated Microbiome in Lung Adenocarcinoma and Lung Squamous Cell Carcinoma Cancers lung adenocarcinoma lung squamous cell carcinoma microbiome age gender TCGA |
| title | Comparative Analysis of Age- and Gender-Associated Microbiome in Lung Adenocarcinoma and Lung Squamous Cell Carcinoma |
| title_full | Comparative Analysis of Age- and Gender-Associated Microbiome in Lung Adenocarcinoma and Lung Squamous Cell Carcinoma |
| title_fullStr | Comparative Analysis of Age- and Gender-Associated Microbiome in Lung Adenocarcinoma and Lung Squamous Cell Carcinoma |
| title_full_unstemmed | Comparative Analysis of Age- and Gender-Associated Microbiome in Lung Adenocarcinoma and Lung Squamous Cell Carcinoma |
| title_short | Comparative Analysis of Age- and Gender-Associated Microbiome in Lung Adenocarcinoma and Lung Squamous Cell Carcinoma |
| title_sort | comparative analysis of age and gender associated microbiome in lung adenocarcinoma and lung squamous cell carcinoma |
| topic | lung adenocarcinoma lung squamous cell carcinoma microbiome age gender TCGA |
| url | https://www.mdpi.com/2072-6694/12/6/1447 |
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