β-Caryophyllene from Chilli Pepper Inhibits the Proliferation of Non-Small Cell Lung Cancer Cells by Affecting miR-659-3p-Targeted Sphingosine Kinase 1 (SphK1)
Jiaji Lei, Qiushi Wang, Guanghua Li, Yongchao Li, Pengfei Zhang, Guangquan Xu Department of Second Ward of Thoracic Surgery, The Second Affiliated Hospital of Harbin Medical University, Harbin, 157011, People’s Republic of ChinaCorrespondence: Guangquan XuDepartment of Second Ward of Thoracic Surger...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Dove Medical Press
2021-12-01
|
| Series: | International Journal of General Medicine |
| Subjects: | |
| Online Access: | https://www.dovepress.com/-caryophyllene-from-chilli-pepper-inhibits-the-proliferation-of-non-sm-peer-reviewed-fulltext-article-IJGM |
| _version_ | 1818700837306761216 |
|---|---|
| author | Lei J Wang Q Li G Li Y Zhang P Xu G |
| author_facet | Lei J Wang Q Li G Li Y Zhang P Xu G |
| author_sort | Lei J |
| collection | DOAJ |
| description | Jiaji Lei, Qiushi Wang, Guanghua Li, Yongchao Li, Pengfei Zhang, Guangquan Xu Department of Second Ward of Thoracic Surgery, The Second Affiliated Hospital of Harbin Medical University, Harbin, 157011, People’s Republic of ChinaCorrespondence: Guangquan XuDepartment of Second Ward of Thoracic Surgery, The Second Affiliated Hospital of Harbin Medical University, No. 246 Baojian Road, Nangang District, Harbin, 157011, People’s Republic of ChinaTel/Fax +86- 0451-86297241Email xuguang12quan@163.comBackground: β-Caryophyllene is the main ingredient of chilli pepper and used for the prevention of various cancers, while the molecular mechanism for its effects on non-small cell lung cancer (NSCLC) remains unclear.Methods: NSCLC cell lines A549 and NCI-H1299 were treated with β-Caryophyllene and miR-659-3p (a potential tumor suppressor) mimic or siRNA. The levels of miR-659-3p, sphingosine kinase 1 (SphK1), apoptotic factors and oxidative stress factors were investigated.Results: β-Caryophyllene inhibited NSCLC growth, promoted their apoptotic rate, increased the level of miR-659-3p, apoptotic factors (cleaved caspase-3 and BAX), antioxidant factors (SOD, CAT and GPx) and reduced the level of oxidative stress (ROS and NO) and SphK1. miR-659-3p mimic and siRNA affected NSCLC growth, their apoptosis, and biochemical indices.Conclusion: β-Caryophyllene of chilli pepper exerts inhibitory activity in NSCLC cells possibly by affecting miR-659-3p-targeted SphK1.Keywords: β-caryophyllene, chilli pepper, non-small cell lung cancer, miR-659-3p, sphingosine kinase 1 |
| first_indexed | 2024-12-17T15:11:17Z |
| format | Article |
| id | doaj.art-079d3eefdd6e465bb4c4f0262a4977e0 |
| institution | Directory Open Access Journal |
| issn | 1178-7074 |
| language | English |
| last_indexed | 2024-12-17T15:11:17Z |
| publishDate | 2021-12-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | International Journal of General Medicine |
| spelling | doaj.art-079d3eefdd6e465bb4c4f0262a4977e02022-12-21T21:43:39ZengDove Medical PressInternational Journal of General Medicine1178-70742021-12-01Volume 149599961371356β-Caryophyllene from Chilli Pepper Inhibits the Proliferation of Non-Small Cell Lung Cancer Cells by Affecting miR-659-3p-Targeted Sphingosine Kinase 1 (SphK1)Lei JWang QLi GLi YZhang PXu GJiaji Lei, Qiushi Wang, Guanghua Li, Yongchao Li, Pengfei Zhang, Guangquan Xu Department of Second Ward of Thoracic Surgery, The Second Affiliated Hospital of Harbin Medical University, Harbin, 157011, People’s Republic of ChinaCorrespondence: Guangquan XuDepartment of Second Ward of Thoracic Surgery, The Second Affiliated Hospital of Harbin Medical University, No. 246 Baojian Road, Nangang District, Harbin, 157011, People’s Republic of ChinaTel/Fax +86- 0451-86297241Email xuguang12quan@163.comBackground: β-Caryophyllene is the main ingredient of chilli pepper and used for the prevention of various cancers, while the molecular mechanism for its effects on non-small cell lung cancer (NSCLC) remains unclear.Methods: NSCLC cell lines A549 and NCI-H1299 were treated with β-Caryophyllene and miR-659-3p (a potential tumor suppressor) mimic or siRNA. The levels of miR-659-3p, sphingosine kinase 1 (SphK1), apoptotic factors and oxidative stress factors were investigated.Results: β-Caryophyllene inhibited NSCLC growth, promoted their apoptotic rate, increased the level of miR-659-3p, apoptotic factors (cleaved caspase-3 and BAX), antioxidant factors (SOD, CAT and GPx) and reduced the level of oxidative stress (ROS and NO) and SphK1. miR-659-3p mimic and siRNA affected NSCLC growth, their apoptosis, and biochemical indices.Conclusion: β-Caryophyllene of chilli pepper exerts inhibitory activity in NSCLC cells possibly by affecting miR-659-3p-targeted SphK1.Keywords: β-caryophyllene, chilli pepper, non-small cell lung cancer, miR-659-3p, sphingosine kinase 1https://www.dovepress.com/-caryophyllene-from-chilli-pepper-inhibits-the-proliferation-of-non-sm-peer-reviewed-fulltext-article-IJGMβ-caryophyllenechilli peppernon-small cell lung cancermir-659-3psphingosine kinase 1 |
| spellingShingle | Lei J Wang Q Li G Li Y Zhang P Xu G β-Caryophyllene from Chilli Pepper Inhibits the Proliferation of Non-Small Cell Lung Cancer Cells by Affecting miR-659-3p-Targeted Sphingosine Kinase 1 (SphK1) International Journal of General Medicine β-caryophyllene chilli pepper non-small cell lung cancer mir-659-3p sphingosine kinase 1 |
| title | β-Caryophyllene from Chilli Pepper Inhibits the Proliferation of Non-Small Cell Lung Cancer Cells by Affecting miR-659-3p-Targeted Sphingosine Kinase 1 (SphK1) |
| title_full | β-Caryophyllene from Chilli Pepper Inhibits the Proliferation of Non-Small Cell Lung Cancer Cells by Affecting miR-659-3p-Targeted Sphingosine Kinase 1 (SphK1) |
| title_fullStr | β-Caryophyllene from Chilli Pepper Inhibits the Proliferation of Non-Small Cell Lung Cancer Cells by Affecting miR-659-3p-Targeted Sphingosine Kinase 1 (SphK1) |
| title_full_unstemmed | β-Caryophyllene from Chilli Pepper Inhibits the Proliferation of Non-Small Cell Lung Cancer Cells by Affecting miR-659-3p-Targeted Sphingosine Kinase 1 (SphK1) |
| title_short | β-Caryophyllene from Chilli Pepper Inhibits the Proliferation of Non-Small Cell Lung Cancer Cells by Affecting miR-659-3p-Targeted Sphingosine Kinase 1 (SphK1) |
| title_sort | beta caryophyllene from chilli pepper inhibits the proliferation of non small cell lung cancer cells by affecting mir 659 3p targeted sphingosine kinase 1 sphk1 |
| topic | β-caryophyllene chilli pepper non-small cell lung cancer mir-659-3p sphingosine kinase 1 |
| url | https://www.dovepress.com/-caryophyllene-from-chilli-pepper-inhibits-the-proliferation-of-non-sm-peer-reviewed-fulltext-article-IJGM |
| work_keys_str_mv | AT leij betacaryophyllenefromchillipepperinhibitstheproliferationofnonsmallcelllungcancercellsbyaffectingmir6593ptargetedsphingosinekinase1sphk1 AT wangq betacaryophyllenefromchillipepperinhibitstheproliferationofnonsmallcelllungcancercellsbyaffectingmir6593ptargetedsphingosinekinase1sphk1 AT lig betacaryophyllenefromchillipepperinhibitstheproliferationofnonsmallcelllungcancercellsbyaffectingmir6593ptargetedsphingosinekinase1sphk1 AT liy betacaryophyllenefromchillipepperinhibitstheproliferationofnonsmallcelllungcancercellsbyaffectingmir6593ptargetedsphingosinekinase1sphk1 AT zhangp betacaryophyllenefromchillipepperinhibitstheproliferationofnonsmallcelllungcancercellsbyaffectingmir6593ptargetedsphingosinekinase1sphk1 AT xug betacaryophyllenefromchillipepperinhibitstheproliferationofnonsmallcelllungcancercellsbyaffectingmir6593ptargetedsphingosinekinase1sphk1 |