Rapid discrimination of human oesophageal squamous cell carcinoma by mass spectrometry based on differences in amino acid metabolism
Abstract Oesophageal cancer (OC) is associated with high morbidity and mortality, and surgery is the most effective approach to treat it. In order to reduce surgical risks and duration of surgery, we explored a new strategy to determine tumour margins in surgery. In this study, we included 128 cance...
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| Format: | Article |
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Nature Portfolio
2017-06-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-017-03375-8 |
| _version_ | 1818844115731742720 |
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| author | Zhang Jianyong Xu Jianjun Ouyang Yongzhong Liu Junwen Lu Haiyan Yu Dongliang Peng Jinhua Xiong Junwen Chen Huanwen Wei Yiping |
| author_facet | Zhang Jianyong Xu Jianjun Ouyang Yongzhong Liu Junwen Lu Haiyan Yu Dongliang Peng Jinhua Xiong Junwen Chen Huanwen Wei Yiping |
| author_sort | Zhang Jianyong |
| collection | DOAJ |
| description | Abstract Oesophageal cancer (OC) is associated with high morbidity and mortality, and surgery is the most effective approach to treat it. In order to reduce surgical risks and duration of surgery, we explored a new strategy to determine tumour margins in surgery. In this study, we included 128 cancerous and 128 noncancerous database entries obtained from 32 human patients. Using internal extractive electrospray ionization-MS, in positive ion detection mode, the relative abundances of m/z 104.13, m/z 116.10, m/z 132.13, and m/z 175.13 were higher in cancer tissue while the relative abundances of m/z 82.99, m/z 133.11, m/z 147.08, m/z 154.06, and m/z 188.05 were higher in normal tissue. Using partial least squares analysis, the mass spectra of cancer samples was discriminated from those of normal tissues, and the discriminatory ions were obtained from loading plots. Dimethylglycine(m/z 104), proline(m/z 116), isoleucine(m/z 132), asparagine(m/z 133), glutamine(m/z 147), and arginine(m/z 175) were identified by collision-induced dissociation experiments. Using the ROC curve analysis, we verified the validity of six amino acids for the identification of tumour tissue. Further investigations of tissue amino acids may allow us to better understand the underlying mechanisms involved in OC and develop novel means to identify tumour tissue during operation. |
| first_indexed | 2024-12-19T05:08:38Z |
| format | Article |
| id | doaj.art-079d89417759437db8317e4cad4d4348 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-19T05:08:38Z |
| publishDate | 2017-06-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-079d89417759437db8317e4cad4d43482022-12-21T20:34:51ZengNature PortfolioScientific Reports2045-23222017-06-01711910.1038/s41598-017-03375-8Rapid discrimination of human oesophageal squamous cell carcinoma by mass spectrometry based on differences in amino acid metabolismZhang Jianyong0Xu Jianjun1Ouyang Yongzhong2Liu Junwen3Lu Haiyan4Yu Dongliang5Peng Jinhua6Xiong Junwen7Chen Huanwen8Wei Yiping9Department of Cardiothoracic Surgery, the Second Affiliated Hospital of Nanchang UniversityDepartment of Cardiothoracic Surgery, the Second Affiliated Hospital of Nanchang UniversitySchool of Chemistry, Biology and Materials Science, East China University of TechnologySchool of Chemistry, Biology and Materials Science, East China University of TechnologyJiangxi Key Laboratory for Mass Spectrometry and Instrumentation, East China University of TechnologyDepartment of Cardiothoracic Surgery, the Second Affiliated Hospital of Nanchang UniversityDepartment of Cardiothoracic Surgery, the Second Affiliated Hospital of Nanchang UniversityDepartment of Cardiothoracic Surgery, the Second Affiliated Hospital of Nanchang UniversityJiangxi Key Laboratory for Mass Spectrometry and Instrumentation, East China University of TechnologyDepartment of Cardiothoracic Surgery, the Second Affiliated Hospital of Nanchang UniversityAbstract Oesophageal cancer (OC) is associated with high morbidity and mortality, and surgery is the most effective approach to treat it. In order to reduce surgical risks and duration of surgery, we explored a new strategy to determine tumour margins in surgery. In this study, we included 128 cancerous and 128 noncancerous database entries obtained from 32 human patients. Using internal extractive electrospray ionization-MS, in positive ion detection mode, the relative abundances of m/z 104.13, m/z 116.10, m/z 132.13, and m/z 175.13 were higher in cancer tissue while the relative abundances of m/z 82.99, m/z 133.11, m/z 147.08, m/z 154.06, and m/z 188.05 were higher in normal tissue. Using partial least squares analysis, the mass spectra of cancer samples was discriminated from those of normal tissues, and the discriminatory ions were obtained from loading plots. Dimethylglycine(m/z 104), proline(m/z 116), isoleucine(m/z 132), asparagine(m/z 133), glutamine(m/z 147), and arginine(m/z 175) were identified by collision-induced dissociation experiments. Using the ROC curve analysis, we verified the validity of six amino acids for the identification of tumour tissue. Further investigations of tissue amino acids may allow us to better understand the underlying mechanisms involved in OC and develop novel means to identify tumour tissue during operation.https://doi.org/10.1038/s41598-017-03375-8 |
| spellingShingle | Zhang Jianyong Xu Jianjun Ouyang Yongzhong Liu Junwen Lu Haiyan Yu Dongliang Peng Jinhua Xiong Junwen Chen Huanwen Wei Yiping Rapid discrimination of human oesophageal squamous cell carcinoma by mass spectrometry based on differences in amino acid metabolism Scientific Reports |
| title | Rapid discrimination of human oesophageal squamous cell carcinoma by mass spectrometry based on differences in amino acid metabolism |
| title_full | Rapid discrimination of human oesophageal squamous cell carcinoma by mass spectrometry based on differences in amino acid metabolism |
| title_fullStr | Rapid discrimination of human oesophageal squamous cell carcinoma by mass spectrometry based on differences in amino acid metabolism |
| title_full_unstemmed | Rapid discrimination of human oesophageal squamous cell carcinoma by mass spectrometry based on differences in amino acid metabolism |
| title_short | Rapid discrimination of human oesophageal squamous cell carcinoma by mass spectrometry based on differences in amino acid metabolism |
| title_sort | rapid discrimination of human oesophageal squamous cell carcinoma by mass spectrometry based on differences in amino acid metabolism |
| url | https://doi.org/10.1038/s41598-017-03375-8 |
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