Characterization of Bottlenose Dolphin <i>(Tursiops truncatus)</i> Sperm Based on Morphometric Traits
Bottlenose dolphin (<i>Tursiops truncatus</i>) males follow many reproductive strategies to ensure their paternity. However, little is known about the sperm traits, including morphometric features, that contribute to their reproductive success. Our aim was to study dolphin sperm morphome...
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MDPI AG
2021-04-01
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| author | María del Carmen Fuentes-Albero Silvia Abril Sánchez José Luis Ros-Santaella Eliana Pintus Chiara Luongo Sara Ruiz Díaz Carlos Barros García María Jesús Sánchez Calabuig Daniel García Párraga Francisco Alberto García Vázquez |
| author_facet | María del Carmen Fuentes-Albero Silvia Abril Sánchez José Luis Ros-Santaella Eliana Pintus Chiara Luongo Sara Ruiz Díaz Carlos Barros García María Jesús Sánchez Calabuig Daniel García Párraga Francisco Alberto García Vázquez |
| author_sort | María del Carmen Fuentes-Albero |
| collection | DOAJ |
| description | Bottlenose dolphin (<i>Tursiops truncatus</i>) males follow many reproductive strategies to ensure their paternity. However, little is known about the sperm traits, including morphometric features, that contribute to their reproductive success. Our aim was to study dolphin sperm morphometry (a total of 13 parameters) in two adult males to evaluate (i) presumptive sperm subpopulations, (ii) the correlation of sperm morphometry with testosterone levels and (iii) the effect of refrigerated storage on the sperm morphometry. Sperm populations were classified into four principal components (PCs) based on morphometry (>94% of cumulative variance). The PCs clustered into two different sperm subpopulations, which differed between males. Furthermore, the levels of serum testosterone were positively correlated with the length of the midpiece but negatively correlated with head width and the principal piece, flagellum and total sperm lengths. Most of the sperm morphometric parameters changed during the storage period (day 1 vs. day 7), but only the principal piece length was affected by the storage temperature (5 °C vs. 15 °C). This is the first study to identify dolphin sperm subpopulations based on morphometry and the influence of serum testosterone and refrigeration on sperm morphometry. |
| first_indexed | 2024-03-10T12:04:22Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2079-7737 |
| language | English |
| last_indexed | 2024-03-10T12:04:22Z |
| publishDate | 2021-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biology |
| spelling | doaj.art-079feeb50fc0479c9411688ae213c68d2023-11-21T16:39:36ZengMDPI AGBiology2079-77372021-04-0110535510.3390/biology10050355Characterization of Bottlenose Dolphin <i>(Tursiops truncatus)</i> Sperm Based on Morphometric TraitsMaría del Carmen Fuentes-Albero0Silvia Abril Sánchez1José Luis Ros-Santaella2Eliana Pintus3Chiara Luongo4Sara Ruiz Díaz5Carlos Barros García6María Jesús Sánchez Calabuig7Daniel García Párraga8Francisco Alberto García Vázquez9Biology Department, Avanqua-Oceanogràfic S.L., 46013 Valencia, SpainDepartment of Physiology, Faculty of Veterinary Science, Campus Mare Nostrum, University of Murcia, 30100 Murcia, SpainDepartment of Veterinary Sciences, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Kamýcká 129, 165 00 Prague, Czech RepublicDepartment of Veterinary Sciences, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Kamýcká 129, 165 00 Prague, Czech RepublicDepartment of Physiology, Faculty of Veterinary Science, Campus Mare Nostrum, University of Murcia, 30100 Murcia, SpainDepartment of Animal Reproduction, INIA, 28040 Madrid, SpainBiology Department, Avanqua-Oceanogràfic S.L., 46013 Valencia, SpainDepartment of Animal Reproduction, INIA, 28040 Madrid, SpainBiology Department, Avanqua-Oceanogràfic S.L., 46013 Valencia, SpainDepartment of Physiology, Faculty of Veterinary Science, Campus Mare Nostrum, University of Murcia, 30100 Murcia, SpainBottlenose dolphin (<i>Tursiops truncatus</i>) males follow many reproductive strategies to ensure their paternity. However, little is known about the sperm traits, including morphometric features, that contribute to their reproductive success. Our aim was to study dolphin sperm morphometry (a total of 13 parameters) in two adult males to evaluate (i) presumptive sperm subpopulations, (ii) the correlation of sperm morphometry with testosterone levels and (iii) the effect of refrigerated storage on the sperm morphometry. Sperm populations were classified into four principal components (PCs) based on morphometry (>94% of cumulative variance). The PCs clustered into two different sperm subpopulations, which differed between males. Furthermore, the levels of serum testosterone were positively correlated with the length of the midpiece but negatively correlated with head width and the principal piece, flagellum and total sperm lengths. Most of the sperm morphometric parameters changed during the storage period (day 1 vs. day 7), but only the principal piece length was affected by the storage temperature (5 °C vs. 15 °C). This is the first study to identify dolphin sperm subpopulations based on morphometry and the influence of serum testosterone and refrigeration on sperm morphometry.https://www.mdpi.com/2079-7737/10/5/355cetaceanmorphologymorphometrysemensperm cells |
| spellingShingle | María del Carmen Fuentes-Albero Silvia Abril Sánchez José Luis Ros-Santaella Eliana Pintus Chiara Luongo Sara Ruiz Díaz Carlos Barros García María Jesús Sánchez Calabuig Daniel García Párraga Francisco Alberto García Vázquez Characterization of Bottlenose Dolphin <i>(Tursiops truncatus)</i> Sperm Based on Morphometric Traits Biology cetacean morphology morphometry semen sperm cells |
| title | Characterization of Bottlenose Dolphin <i>(Tursiops truncatus)</i> Sperm Based on Morphometric Traits |
| title_full | Characterization of Bottlenose Dolphin <i>(Tursiops truncatus)</i> Sperm Based on Morphometric Traits |
| title_fullStr | Characterization of Bottlenose Dolphin <i>(Tursiops truncatus)</i> Sperm Based on Morphometric Traits |
| title_full_unstemmed | Characterization of Bottlenose Dolphin <i>(Tursiops truncatus)</i> Sperm Based on Morphometric Traits |
| title_short | Characterization of Bottlenose Dolphin <i>(Tursiops truncatus)</i> Sperm Based on Morphometric Traits |
| title_sort | characterization of bottlenose dolphin i tursiops truncatus i sperm based on morphometric traits |
| topic | cetacean morphology morphometry semen sperm cells |
| url | https://www.mdpi.com/2079-7737/10/5/355 |
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