Isolation and Characterization of New Bacteriophages against Staphylococcal Clinical Isolates from Diabetic Foot Ulcers
<i>Staphylococcus</i> sp. is the most common bacterial genus in infections related to diabetic foot ulcers (DFUs). The emergence of multidrug-resistant bacteria places a serious burden on public health systems. Phage therapy is an alternative treatment to antibiotics, overcoming the issu...
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MDPI AG
2023-11-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/15/12/2287 |
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| author | Lucile Plumet Madjid Morsli Nour Ahmad-Mansour Fernando Clavijo-Coppens Laurence Berry Albert Sotto Jean-Philippe Lavigne Denis Costechareyre Virginie Molle |
| author_facet | Lucile Plumet Madjid Morsli Nour Ahmad-Mansour Fernando Clavijo-Coppens Laurence Berry Albert Sotto Jean-Philippe Lavigne Denis Costechareyre Virginie Molle |
| author_sort | Lucile Plumet |
| collection | DOAJ |
| description | <i>Staphylococcus</i> sp. is the most common bacterial genus in infections related to diabetic foot ulcers (DFUs). The emergence of multidrug-resistant bacteria places a serious burden on public health systems. Phage therapy is an alternative treatment to antibiotics, overcoming the issue of antibiotic resistance. In this study, six phages (SAVM01 to SAVM06) were isolated from effluents and were used against a panel of staphylococcal clinical samples isolated from DFUs. A genomic analysis revealed that the phages belonged to the <i>Herelleviridae</i> family, with sequences similar to those of the <i>Kayvirus</i> genus. No lysogeny-associated genes, known virulence or drug resistance genes were identified in the phage genomes. The phages displayed a strong lytic and antibiofilm activity against DFU clinical isolates, as well as against opportunistic pathogenic coagulase-negative staphylococci. The results presented here suggest that these phages could be effective biocontrol agents against staphylococcal clinical isolates from DFUs. |
| first_indexed | 2024-03-08T20:17:44Z |
| format | Article |
| id | doaj.art-07a2401831884f11b16559227ee29f84 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-08T20:17:44Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-07a2401831884f11b16559227ee29f842023-12-22T14:48:57ZengMDPI AGViruses1999-49152023-11-011512228710.3390/v15122287Isolation and Characterization of New Bacteriophages against Staphylococcal Clinical Isolates from Diabetic Foot UlcersLucile Plumet0Madjid Morsli1Nour Ahmad-Mansour2Fernando Clavijo-Coppens3Laurence Berry4Albert Sotto5Jean-Philippe Lavigne6Denis Costechareyre7Virginie Molle8VBIC, INSERM U1047, University of Montpellier, 34095 Montpellier, FranceVBIC, INSERM U1047, Department of Microbiology and Hospital Hygiene, University of Montpellier, CHU Nîmes, 30908 Nîmes, FranceVBIC, INSERM U1047, University of Montpellier, 34095 Montpellier, FranceGreenphage, Cap Alpha, 34830 Clapiers, FranceLaboratory of Pathogen and Host Immunity, CNRS UMR5294, University of Montpellier, 34095 Montpellier, FranceVBIC, INSERM U1047, Department of Infectious Diseases, University of de Montpellier, CHU Nîmes, 30908 Nîmes, FranceVBIC, INSERM U1047, Department of Microbiology and Hospital Hygiene, University of Montpellier, CHU Nîmes, 30908 Nîmes, FranceGreenphage, Cap Alpha, 34830 Clapiers, FranceVBIC, INSERM U1047, University of Montpellier, 34095 Montpellier, France<i>Staphylococcus</i> sp. is the most common bacterial genus in infections related to diabetic foot ulcers (DFUs). The emergence of multidrug-resistant bacteria places a serious burden on public health systems. Phage therapy is an alternative treatment to antibiotics, overcoming the issue of antibiotic resistance. In this study, six phages (SAVM01 to SAVM06) were isolated from effluents and were used against a panel of staphylococcal clinical samples isolated from DFUs. A genomic analysis revealed that the phages belonged to the <i>Herelleviridae</i> family, with sequences similar to those of the <i>Kayvirus</i> genus. No lysogeny-associated genes, known virulence or drug resistance genes were identified in the phage genomes. The phages displayed a strong lytic and antibiofilm activity against DFU clinical isolates, as well as against opportunistic pathogenic coagulase-negative staphylococci. The results presented here suggest that these phages could be effective biocontrol agents against staphylococcal clinical isolates from DFUs.https://www.mdpi.com/1999-4915/15/12/2287bacteriophagesbiofilmdiabetic foot ulcer<i>Kayvirus</i><i>Staphylococcus</i> sp. |
| spellingShingle | Lucile Plumet Madjid Morsli Nour Ahmad-Mansour Fernando Clavijo-Coppens Laurence Berry Albert Sotto Jean-Philippe Lavigne Denis Costechareyre Virginie Molle Isolation and Characterization of New Bacteriophages against Staphylococcal Clinical Isolates from Diabetic Foot Ulcers Viruses bacteriophages biofilm diabetic foot ulcer <i>Kayvirus</i> <i>Staphylococcus</i> sp. |
| title | Isolation and Characterization of New Bacteriophages against Staphylococcal Clinical Isolates from Diabetic Foot Ulcers |
| title_full | Isolation and Characterization of New Bacteriophages against Staphylococcal Clinical Isolates from Diabetic Foot Ulcers |
| title_fullStr | Isolation and Characterization of New Bacteriophages against Staphylococcal Clinical Isolates from Diabetic Foot Ulcers |
| title_full_unstemmed | Isolation and Characterization of New Bacteriophages against Staphylococcal Clinical Isolates from Diabetic Foot Ulcers |
| title_short | Isolation and Characterization of New Bacteriophages against Staphylococcal Clinical Isolates from Diabetic Foot Ulcers |
| title_sort | isolation and characterization of new bacteriophages against staphylococcal clinical isolates from diabetic foot ulcers |
| topic | bacteriophages biofilm diabetic foot ulcer <i>Kayvirus</i> <i>Staphylococcus</i> sp. |
| url | https://www.mdpi.com/1999-4915/15/12/2287 |
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