Emergence of Raoultella ornithinolytica isolated from chicken products in Alexandria, Egypt
Background and Aim: Raoultella ornithinolytica is one of the emerging gram-negative bacteria, which associated with foodborne illness. Researches affirmed that distinguish between R. ornithinolytica and Klebsiella oxytoca are difficult, as they are phylogenetic related. The evolution of multidrug re...
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| Format: | Article |
| Language: | English |
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Veterinary World
2020-07-01
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| Series: | Veterinary World |
| Subjects: | |
| Online Access: | http://www.veterinaryworld.org/Vol.13/July-2020/30.pdf |
| _version_ | 1818590640141762560 |
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| author | Sara M. El-Shannat Ashraf A. Abd El-Tawab Wafaa M. M. Hassan |
| author_facet | Sara M. El-Shannat Ashraf A. Abd El-Tawab Wafaa M. M. Hassan |
| author_sort | Sara M. El-Shannat |
| collection | DOAJ |
| description | Background and Aim: Raoultella ornithinolytica is one of the emerging gram-negative bacteria, which associated with foodborne illness. Researches affirmed that distinguish between R. ornithinolytica and Klebsiella oxytoca are difficult, as they are phylogenetic related. The evolution of multidrug resistance of Raoultella strains gained more concern for recognition of the pathogen which supports in controlling the disease and minify its threat. This study sought to find a reliable tool for the identification of Raoultella ornithinolytica, isolated from chicken product samples, and assessed the resistance profile of R. ornithinolytica using antibiogram sensitivity tests.
Materials and Methods: Forty samples of chicken products were collected between January and September 2019 from different markets in Alexandria Governorate, Egypt. The products included nuggets, strips, burgers, luncheon meats, pane, frankfurters, and minced chicken meat. The samples were transferred to the Reference Laboratory. The samples were subjected to isolation, biochemical reaction testing, phenotypic system analytical profile index (API) E20, and a detection of antimicrobial susceptibility test. Phenotypic identification was confirmed through matrix-assisted laser-desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS).
Results: Thirty-three bacterial isolates (82.50%) out of 40 samples were isolated into pure cultures from the chicken samples. Three isolates (9.09%) were positive for R. ornithinolytica, while 30 isolates (90.91%) exhibited growth characters for different pathogens (Escherichia coli, Enterobacter aerogenes, Proteus vulgaris, R. ornithinolytica, and Klebsiella pneumoniae). The isolates of R. ornithinolytica were resistant to five types of antibiotics and sensitive to two types of antibiotics.
Conclusion: This study reported the first case of R. ornithinolytica found in chicken products in Egypt. Phenotypic system API 20E and MALDI-TOF MS were found to be reliable tools for confirming the diagnosis of R. ornithinolytica. As it provides rapid identification with high sensitivity and specificity for R. ornithinolytica, which often do not require a molecular procedure for confirmation. |
| first_indexed | 2024-12-16T09:59:45Z |
| format | Article |
| id | doaj.art-07a43b7d7fb848e1b0b4947693b5315a |
| institution | Directory Open Access Journal |
| issn | 0972-8988 2231-0916 |
| language | English |
| last_indexed | 2024-12-16T09:59:45Z |
| publishDate | 2020-07-01 |
| publisher | Veterinary World |
| record_format | Article |
| series | Veterinary World |
| spelling | doaj.art-07a43b7d7fb848e1b0b4947693b5315a2022-12-21T22:35:49ZengVeterinary WorldVeterinary World0972-89882231-09162020-07-011371473147910.14202/vetworld.2020.1473-1479Emergence of Raoultella ornithinolytica isolated from chicken products in Alexandria, EgyptSara M. El-Shannat0https://orcid.org/0000-0002-2693-9054Ashraf A. Abd El-Tawab1https://orcid.org/0000-0002-1221-3340Wafaa M. M. Hassan2Department of Microbiology, Animal Health Research Institute, Marsa Matruh, Egypt.Department of Bacteriology, Immunology, and Mycology, Faculty of Veterinary Medicine, Benha University, Banha, Egypt.The Reference Laboratory for Quality Control on Poultry Production, Animal Health Research Institute, Dokki, Giza, Egypt.Background and Aim: Raoultella ornithinolytica is one of the emerging gram-negative bacteria, which associated with foodborne illness. Researches affirmed that distinguish between R. ornithinolytica and Klebsiella oxytoca are difficult, as they are phylogenetic related. The evolution of multidrug resistance of Raoultella strains gained more concern for recognition of the pathogen which supports in controlling the disease and minify its threat. This study sought to find a reliable tool for the identification of Raoultella ornithinolytica, isolated from chicken product samples, and assessed the resistance profile of R. ornithinolytica using antibiogram sensitivity tests. Materials and Methods: Forty samples of chicken products were collected between January and September 2019 from different markets in Alexandria Governorate, Egypt. The products included nuggets, strips, burgers, luncheon meats, pane, frankfurters, and minced chicken meat. The samples were transferred to the Reference Laboratory. The samples were subjected to isolation, biochemical reaction testing, phenotypic system analytical profile index (API) E20, and a detection of antimicrobial susceptibility test. Phenotypic identification was confirmed through matrix-assisted laser-desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS). Results: Thirty-three bacterial isolates (82.50%) out of 40 samples were isolated into pure cultures from the chicken samples. Three isolates (9.09%) were positive for R. ornithinolytica, while 30 isolates (90.91%) exhibited growth characters for different pathogens (Escherichia coli, Enterobacter aerogenes, Proteus vulgaris, R. ornithinolytica, and Klebsiella pneumoniae). The isolates of R. ornithinolytica were resistant to five types of antibiotics and sensitive to two types of antibiotics. Conclusion: This study reported the first case of R. ornithinolytica found in chicken products in Egypt. Phenotypic system API 20E and MALDI-TOF MS were found to be reliable tools for confirming the diagnosis of R. ornithinolytica. As it provides rapid identification with high sensitivity and specificity for R. ornithinolytica, which often do not require a molecular procedure for confirmation.http://www.veterinaryworld.org/Vol.13/July-2020/30.pdfanalytical profile index 20ematrix-assisted laser-desorption ionization time-of-flight mass spectrometryphenotypic systemraoultella ornithinolytica |
| spellingShingle | Sara M. El-Shannat Ashraf A. Abd El-Tawab Wafaa M. M. Hassan Emergence of Raoultella ornithinolytica isolated from chicken products in Alexandria, Egypt Veterinary World analytical profile index 20e matrix-assisted laser-desorption ionization time-of-flight mass spectrometry phenotypic system raoultella ornithinolytica |
| title | Emergence of Raoultella ornithinolytica isolated from chicken products in Alexandria, Egypt |
| title_full | Emergence of Raoultella ornithinolytica isolated from chicken products in Alexandria, Egypt |
| title_fullStr | Emergence of Raoultella ornithinolytica isolated from chicken products in Alexandria, Egypt |
| title_full_unstemmed | Emergence of Raoultella ornithinolytica isolated from chicken products in Alexandria, Egypt |
| title_short | Emergence of Raoultella ornithinolytica isolated from chicken products in Alexandria, Egypt |
| title_sort | emergence of raoultella ornithinolytica isolated from chicken products in alexandria egypt |
| topic | analytical profile index 20e matrix-assisted laser-desorption ionization time-of-flight mass spectrometry phenotypic system raoultella ornithinolytica |
| url | http://www.veterinaryworld.org/Vol.13/July-2020/30.pdf |
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