Genome-wide analysis of the laccase (LAC) gene family in Aeluropus littoralis: A focus on identification, evolution and expression patterns in response to abiotic stresses and ABA treatment
Laccases are plant enzymes with essential functions during growth and development. These monophenoloxidases are involved in lignin polymerization, and their expression respond to environmental stress. However, studies of laccases in some plants and fungi have highlighted that many structural and fun...
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Frontiers Media S.A.
2023-03-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2023.1112354/full |
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| author | Seyyed Hamidreza Hashemipetroudi Seyyed Hamidreza Hashemipetroudi Mozhdeh Arab Mozhdeh Arab Parviz Heidari Markus Kuhlmann |
| author_facet | Seyyed Hamidreza Hashemipetroudi Seyyed Hamidreza Hashemipetroudi Mozhdeh Arab Mozhdeh Arab Parviz Heidari Markus Kuhlmann |
| author_sort | Seyyed Hamidreza Hashemipetroudi |
| collection | DOAJ |
| description | Laccases are plant enzymes with essential functions during growth and development. These monophenoloxidases are involved in lignin polymerization, and their expression respond to environmental stress. However, studies of laccases in some plants and fungi have highlighted that many structural and functional aspects of these genes are still unknown. Here, the laccase gene family in Aeluropus littoralis (AlLAC) is described based on sequence structure and expression patterns under abiotic stresses and ABA treatment. Fifteen non-redundant AlLACs were identified from the A. littoralis genome, which showed differences in physicochemical characteristics and gene structure. Based on phylogenetic analysis, AlLACs and their orthologues were classified into five groups. A close evolutionary relationship was observed between LAC gene family members in rice and A. littoralis. According to the interaction network, AlLACs interact more with proteins involved in biological processes such as iron incorporation into the metallo-sulfur cluster, lignin catabolism, regulation of the symbiotic process and plant-type primary cell wall biogenesis. Gene expression analysis of selected AlLACs using real-time RT (reverse transcription)-PCR revealed that AlLACs are induced in response to abiotic stresses such as cold, salt, and osmotic stress, as well as ABA treatment. Moreover, AlLACs showed differential expression patterns in shoot and root tissues. Our findings indicate that AlLACs are preferentially involved in the late response of A. littoralis to abiotic stress. |
| first_indexed | 2024-04-10T06:32:31Z |
| format | Article |
| id | doaj.art-07a5742a20d54c72b0692198e2db4836 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-10T06:32:31Z |
| publishDate | 2023-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-07a5742a20d54c72b0692198e2db48362023-03-01T05:09:07ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2023-03-011410.3389/fpls.2023.11123541112354Genome-wide analysis of the laccase (LAC) gene family in Aeluropus littoralis: A focus on identification, evolution and expression patterns in response to abiotic stresses and ABA treatmentSeyyed Hamidreza Hashemipetroudi0Seyyed Hamidreza Hashemipetroudi1Mozhdeh Arab2Mozhdeh Arab3Parviz Heidari4Markus Kuhlmann5Department of Genetic Engineering and Biology, Genetics and Agricultural Biotechnology Institute of Tabarestan (GABIT), Sari Agricultural Sciences and Natural Resources University (SANRU), Sari, IranRG Heterosis, Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Gatersleben, GermanyDepartment of Genetic Engineering and Biology, Genetics and Agricultural Biotechnology Institute of Tabarestan (GABIT), Sari Agricultural Sciences and Natural Resources University (SANRU), Sari, IranNational Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, IranFaculty of Agriculture, Shahrood University of Technology, Shahrood, IranRG Heterosis, Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Gatersleben, GermanyLaccases are plant enzymes with essential functions during growth and development. These monophenoloxidases are involved in lignin polymerization, and their expression respond to environmental stress. However, studies of laccases in some plants and fungi have highlighted that many structural and functional aspects of these genes are still unknown. Here, the laccase gene family in Aeluropus littoralis (AlLAC) is described based on sequence structure and expression patterns under abiotic stresses and ABA treatment. Fifteen non-redundant AlLACs were identified from the A. littoralis genome, which showed differences in physicochemical characteristics and gene structure. Based on phylogenetic analysis, AlLACs and their orthologues were classified into five groups. A close evolutionary relationship was observed between LAC gene family members in rice and A. littoralis. According to the interaction network, AlLACs interact more with proteins involved in biological processes such as iron incorporation into the metallo-sulfur cluster, lignin catabolism, regulation of the symbiotic process and plant-type primary cell wall biogenesis. Gene expression analysis of selected AlLACs using real-time RT (reverse transcription)-PCR revealed that AlLACs are induced in response to abiotic stresses such as cold, salt, and osmotic stress, as well as ABA treatment. Moreover, AlLACs showed differential expression patterns in shoot and root tissues. Our findings indicate that AlLACs are preferentially involved in the late response of A. littoralis to abiotic stress.https://www.frontiersin.org/articles/10.3389/fpls.2023.1112354/fullABA treatmentsalt stresscold stressgene expressiongene structureplant gene family |
| spellingShingle | Seyyed Hamidreza Hashemipetroudi Seyyed Hamidreza Hashemipetroudi Mozhdeh Arab Mozhdeh Arab Parviz Heidari Markus Kuhlmann Genome-wide analysis of the laccase (LAC) gene family in Aeluropus littoralis: A focus on identification, evolution and expression patterns in response to abiotic stresses and ABA treatment Frontiers in Plant Science ABA treatment salt stress cold stress gene expression gene structure plant gene family |
| title | Genome-wide analysis of the laccase (LAC) gene family in Aeluropus littoralis: A focus on identification, evolution and expression patterns in response to abiotic stresses and ABA treatment |
| title_full | Genome-wide analysis of the laccase (LAC) gene family in Aeluropus littoralis: A focus on identification, evolution and expression patterns in response to abiotic stresses and ABA treatment |
| title_fullStr | Genome-wide analysis of the laccase (LAC) gene family in Aeluropus littoralis: A focus on identification, evolution and expression patterns in response to abiotic stresses and ABA treatment |
| title_full_unstemmed | Genome-wide analysis of the laccase (LAC) gene family in Aeluropus littoralis: A focus on identification, evolution and expression patterns in response to abiotic stresses and ABA treatment |
| title_short | Genome-wide analysis of the laccase (LAC) gene family in Aeluropus littoralis: A focus on identification, evolution and expression patterns in response to abiotic stresses and ABA treatment |
| title_sort | genome wide analysis of the laccase lac gene family in aeluropus littoralis a focus on identification evolution and expression patterns in response to abiotic stresses and aba treatment |
| topic | ABA treatment salt stress cold stress gene expression gene structure plant gene family |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2023.1112354/full |
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